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Showing 1 to 20 of 98 for “"Nicotiana benthamiana"”.
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Influenza hemagglutinin expression in Nicotiana tabacum and Nicotiana benthamiana.
… was transformed with the plasmid and both Nicotiana tabacum or Nicotiana benthamiana were transformed. To screen for HA3 expression, mRNA was isolated from first generation plants. Second generation plants were successfully screened for protein production using immunoblots to verify HA3 …
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Monoclonal Antibody Expression and Novel Purification in Nicotiana benthamiana
… patients and drug developers. Plants, such as Nicotiana benthamiana, offer a unique production platform due to their recently found ability to produce large amounts of therapeutic proteins in a quick manner. While production would be simple and cheap, purification would not be due to the …
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Development of West Nile virus candidate vaccines in Nicotiana benthamiana
… on a virulent South African WNV strain using Nicotiana benthamiana as the expression platform. We aimed to develop the first candidate vaccine by exploiting the virus's ability to form noninfectious VLPs by expressing only the WNV membrane (prM – precursor, M – matured) and envelope (E) …
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Coat Protein -Mediated Resistance of Apple Mosaic Virus in Nicotiana Benthamiana
The detection of protein accumulation in transgenic plants carrying the full-length ApMV-CP and conferring resistance to ApMV suggested that transgenic coat protein accumulation was involved in eliciting the ApMV resistance response. Protein accumulation of lower-truncated ApMV-CP did not induce …
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Exploring Non-transgenic CRISPR-Cas9 gRNA Delivery Using Transactivation in Nicotiana benthamiana
… reporter expression. Transient assays in N. benthamiana established the functionality of the system, showing increased reporter levels when the two constructs were co-expressed compared to the activator- reporter construct alone. Stable transgenic N. benthamiana lines of the separate …
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Optimization and characterisation of plant produced Human Papillomavirus pseudovirions in Nicotiana benthamiana
… tumefaciens and then infiltrated into Nicotiana benthamiana along with plant expression vectors encoding the HPV 35 L1 and L2 capsid proteins. Particles were purified by iodixanol density gradient ultracentrifugation and the 27% and 33% fractions of this gradient analysed. Transmission …
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Maize R gene Rxo1 Confers Disease Resistance on Pepper and Nicotiana benthamiana
… Rxo1 into the far-related dicot species Nicotiana benthamiana and pepper, and characterized the Rxo1-mediated disease resistance against Xe strains carrying AvrRxo1 or Xe4428. In addition, we explored the virulence function and mechanism of Xe4428. In the Rxo1-transgenic N. benthamiana, …
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Bioproduction Optimization and Characterization of Hyaluronic Acid in Escherichia coli and Nicotiana benthamiana
… gene transfer to transform Nicotiana benthamiana with hHAS2. To enhance precursor availability, N. benthamiana was further engineered to co-express glmS and glmU, which encode key enzymes involved in HA biosynthesis. Co-expression of these genes resulted in a substantial …
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Development of Rift Valley fever virus candidate vaccines and reagents produced in Nicotiana benthamiana
Rift Valley fever (RVF) is a haemorrhagic fever agent caused by an infection with an enveloped negative-stranded RNA Rift Valley fever virus (RVFV). It belongs to the genus Phlebovirus in the family Bunyaviridae. The virus is spread by infected mosquitoes and affects ruminants and humans, causing …
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Design and production of a candidate universal influenza A vaccine in Nicotiana benthamiana plants
… M2e-presenting virus-like particles (VLPs) in Nicotiana benthamiana plants via Agrobacterium-mediated transient expression. Plants are increasingly being examined as alternative recombinant protein expression systems due to their safety, scalability and rapid production times. Moreover, …
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Expression optimization of a human papillomavirus type 16 therapeutic vaccine candidate in Nicotiana benthamiana leaves
… of the present project was to produce LALF-E7 in Nicotiana benthamiana leaves via Agrobacterium-mediated transient expression, and to optimize its expression, extraction and purification. This was done by expressing LALF-E7 using different expression vectors, testing different subcellular …
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Estudo dos mecanismos envolvidos na adaptabilidade diferencial de dois begomovírus em tomateiro e Nicotiana benthamiana
… Os sintomas induzidos pelo ToYSV em tomateiro e Nicotiana benthamiana são mais severos e surgem mais cedo em comparação aos induzidos pelo ToRMV, o que sugere uma adaptação diferencial de cada vírus aos seus hospedeiros. Neste trabalho, foram conduzidos experimentos com o objetivo de analisar o …
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Novel expression and production of Foot-and-mouth disease virus vaccine candidates in Nicotiana benthamiana
… in the absence of the 3C-protease in the plant Nicotiana benthamiana, albeit in low amounts. This platform does not require high biocontainment facilities for the production of recombinant proteins and VLPs and the process is easily scalable. This study centers mainly on optimisation of the FMDV …
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Engineering the Nicotiana benthamiana secretory pathway for the production of Lujo virus vaccines and diagnostic tools
… a production pipeline in HEK293T cells and N. benthamiana for the protein LUJV GP-C∆TM (Lujo virus glycoprotein precursor without the transmembrane region). The LUJV GP-C∆TM was constructed from gene sequences of the envelope glycoprotein of Lujo virus and then adapted to production in HEK293T …
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The Expression of Shuni Virus Nucleocapsid Protein in Nicotiana benthamiana for Use as a Diagnostic Reagent
… the N protein was transiently expressed in N. benthamiana plants using Agrobacterium tumefaciens-mediated infiltration. The recombinant protein produced underwent purification using nickel affinity chromatography. This led to yields of 2.248 mg of SHUV N protein from 35 plants which gave a …
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Harnessing the manufacturing power of the plant system : production of recombinant human interleukin-2 in Nicotiana benthamiana
… (IL-2) in the green tissues of transgenic Nicotiana benthamiana (tobacco). IL-2 is a biopharmaceutical of great importance as it is the standard immunotherapeutic treatment for late-stage metastatic melanoma and renal cell cancers. Using the whole-plant expression system, rather than …
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Working towards a plant-produced Bovine papillomavirus vaccine: expression of BPV1 virus-like particles and pseudovirions in Nicotiana benthamiana
… vaccine, BPV1 VLPs and PsVs were expressed in N. benthamiana plants as a prelude to producing a candidate prophylactic and therapeutic vaccine. Expression of L1 and L1/L2 VLPs was successful, as was the encapsidation of a reporter gene plasmid into the L1/L2 VLPs, demonstrating the first …
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The Expression of Chikungunya Virus Envelope 2 Glycoprotein Variants in Nicotiana benthamiana for the Development of a Diagnostic Reagent
… of the CHIKV E2 glycoprotein were expressed in Nicotiana benthamiana plants to assess their viability for use in a diagnostic reagent for CHIKV infection. Two versions of a tobacco sp. codon-optimised, 6xhis-tagged CHIKV E2 envelope glycoprotein gene were synthesised and cloned into the plant …
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