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Showing 1 to 20 of 233 for “"Nicotiana"”.
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Influenza hemagglutinin expression in Nicotiana tabacum and Nicotiana benthamiana.
… was transformed with the plasmid and both Nicotiana tabacum or Nicotiana benthamiana were transformed. To screen for HA3 expression, mRNA was isolated from first generation plants. Second generation plants were successfully screened for protein production using immunoblots to verify HA3 …
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Analyse des Transkriptionsfaktors TGA2.1 aus Nicotiana tabacum
Zur Aufklärung der spezifischen Rolle von TGA2.1 sollten transgene TGA2.1-Überexpressionspflanzen sowie TGA2.1-transdominante Suppressor-Linien erzeugt werden. Die subzellullare Lokalisation der transgenen Proteine sollte durch Western-Blot-Analysen und DNA-Bindungsstudien untersucht werden. …
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Subcellular Localization of Nicotiana tabacum TGA Transcription Factors
Die Salicylsäure (SA) ist ein wichtiges Signalmolekül bei der Regulation der pflanzlichen Pathogenabwehr. as-1-ähnliche cis-Elemente in den Promotoren von vielen Abwehrgenen vermitteln SA- und auch Auxin-induzierbare Genexpression. Diese Elemente werden vom ASF-1/SARP-Proteinkomplex erkannt, dessen …
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A Functional Analysis of microRNAs in Nicotiana tabacum
… such as corn, soybeans, rice, and Arabidopsis. Nicotiana tabacum, or cultivated tobacco, is an important economic, agricultural, and research crop that provided approximately $4.4 billion dollars to the United States economy in 2007. Although much genetic research has already been dedicated to …
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Expression of recombinant porcine preprorelaxin in Nicotiana tabacum
… which was codon-optimized for expression in Nicotiana tabacum, under the control of either the "super" promoter or the dual enhanced cauliflower mosaic virus 35S promoter. Eighteen transgenic tobacco plants were generated that were transformed with the above recombinant genes. Preprorelaxin, …
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Expression and Function of the Nicotiana tabacum Aquaporin NtAQP1
Die vorliegende Arbeit zeigt die Korrelation zwischen räumlichem und zeitlichem Expressionsmuster von dem Aquaporin NtAQP1 und seiner Funktion im Wasserhaushalt in planta. Immunologische in situ-Studien deuteten auf eine NtAQP1-Protein-Akkumulation in der Wurzelexodermis und -endodermis, im Cortex, …
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Petal cell shape and flower-pollinator interaction in Nicotiana
… colouration compared to non-conical cells. Nicotiana (Solanaceae) presents a diverse range of petal cell shapes. Interestingly, sister species in at least two phylogenetically distinct clades of the genus have contrasting petal epidermal cell shapes (conical vs. non-conical). This project …
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Monoclonal Antibody Expression and Novel Purification in Nicotiana benthamiana
… patients and drug developers. Plants, such as Nicotiana benthamiana, offer a unique production platform due to their recently found ability to produce large amounts of therapeutic proteins in a quick manner. While production would be simple and cheap, purification would not be due to the …
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Development of West Nile virus candidate vaccines in Nicotiana benthamiana
… on a virulent South African WNV strain using Nicotiana benthamiana as the expression platform. We aimed to develop the first candidate vaccine by exploiting the virus's ability to form noninfectious VLPs by expressing only the WNV membrane (prM – precursor, M – matured) and envelope (E) …
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Characterization of the A/B regulon in tobacco (Nicotiana tabacum)
… 1999. Inducible nicotine production in native Nicotiana as an example of adaptive phenotypic plasticity. Journal of Chem. Ecol. 25: 3-30.
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Pengaruh suhu dan lama penyimpanan terhadap viabilitas benih tembakau (Nicotiana tabacum)
… penyimpanan terhadap viabilitas benih tembakau (Nicotiana tabacum). Penelitian ini dilaksanakan di Laboratorium Fisiologi Tumbuhan Jurusan Biologi Fakultas Sains dan Teknologi UIN Maulana Malik Ibrahim Malang pada bulan Januari - Juni 2011. Penelitian ini merupakan penelitian eksperimental …
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Identification, Characterization, and Abiotic Stress Analysis of microRNAs in Nicotiana tabacum
microRNAs (miRNAs) are non-coding RNAs with short sequences that negatively regulate gene expression at the post-transcriptional levels by either binding to mRNAs for degradation, or by inhibiting protein translation. miRNAs are highly evolutionarily conserved, from lower mosses to higher flowering …
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Coat Protein -Mediated Resistance of Apple Mosaic Virus in Nicotiana Benthamiana
The detection of protein accumulation in transgenic plants carrying the full-length ApMV-CP and conferring resistance to ApMV suggested that transgenic coat protein accumulation was involved in eliciting the ApMV resistance response. Protein accumulation of lower-truncated ApMV-CP did not induce …
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Normoxic and anoxic metabolism of Nicotiana tabacum transformants lacking root nitrate reductase
… roots of hydroponically grown tobacco wildtype (Nicotiana tabacum cv. Gatersleben) and of a transformant (LNR-H) with no nitrate reductase (NR) in the roots but almost normal NR in leaves (based on a nia2-double mutant). As an additional control we used occasionally a 35S-transformant of the same …
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Exploring Non-transgenic CRISPR-Cas9 gRNA Delivery Using Transactivation in Nicotiana benthamiana
Gene editing via CRISPR-Cas9 (Clustered, Regularly Interspaced, Short Palindromic Repeats – CRISPR-associated protein 9) is a powerful tool in biotechnology. The method involves an endonuclease Cas9 forming a complex with a guide RNA (gRNA) that matches a distinct DNA target sequence, causing …
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Optimization and characterisation of plant produced Human Papillomavirus pseudovirions in Nicotiana benthamiana
… tumefaciens and then infiltrated into Nicotiana benthamiana along with plant expression vectors encoding the HPV 35 L1 and L2 capsid proteins. Particles were purified by iodixanol density gradient ultracentrifugation and the 27% and 33% fractions of this gradient analysed. Transmission …
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Studies of in vitro flowering and de novo flowers of Nicotiana tabacum
… examine factors influencing de novo flowering of Nicotiana on 2-3 x 10mm explants consisting of epidermal and 3-6 layers of subjacent cells (thin cell layers, TCLs) and to compare de novo to in vivo flowers. TCLs from short-day and long-day tobacco plants were compared with TCLs from day-neutral …
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Genetic analysis of resistance to Myzus persicae (Sulzer) in Nicotiana tabacum L.
Field experiments with the green peach aphid on tobacco were conducted at Blackstone, Virginia in 1983 and 1984. The objectives were to: 1) confirm and identify source materials resistant to the green peach aphid; 2) study the inheritance of aphid resistance; 3) verify heritability of resistant …
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Maize R gene Rxo1 Confers Disease Resistance on Pepper and Nicotiana benthamiana
… Rxo1 into the far-related dicot species Nicotiana benthamiana and pepper, and characterized the Rxo1-mediated disease resistance against Xe strains carrying AvrRxo1 or Xe4428. In addition, we explored the virulence function and mechanism of Xe4428. In the Rxo1-transgenic N. benthamiana, …
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