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Showing 1 to 20 of 22 for “"Ni-NTA"”.

  1. Novel recombinant protein constructs for improved insulin-like growth factor-1 delivery

    Was, Adam (Adam S.)Massachusetts Institute of Technology2006

    … in bacteria for higher production efficiency. Ni-NTA purified protein was detected in three of the four constructs. These proteins may ultimately be useful for myocardial delivery of IGF-1 to facilitate cardiac repair.

    mit Repository record

  2. Characterization of the Biological Activities of Recombinant Fusion Protein Green Fluorescent Protein/Human Zona Pellucida Protein 3 (GFP/HZP3)

    Lin, Zhiyong2019

    … The fusion protein was partially purified by Ni-NTA affinity column from cell culture medium of stably transfected cells generated from a single cell clone. Immuno-blotting analysis of the fusion protein indicated that the GFP/ZP3 was about 90–92 kD in molecular mass which is close to the sum …

    odu Repository record

  3. Properties of NadV / NatV Proteins in a Pyridine Nucleotide (NAD+) Scavenging System Encoded by Vibriophage KVP40

    Li, Zhiqun2004

    … to be involved in the synthesis and recycling of nicotinamide adenine dinucleotide (NAD+). Comparison with proteins of known function indicates that NadV possesses a nicotinamide phosphoribosyl-transferase domain (NAmPRTase) that converts nicotinamide (NAm) to nicotinamide mononucleotide (NMN). …

    ncsu Repository record

  4. Synthesis and Cytotoxicity Studies of Gold Nanoparticle Systems

    Sosibo, Ndabenhle Mercury Sosibo2010

    … such as carboxyl, hydroxyl, biotin and nitrilotriacetic acid (NTA) were introduced resulting in stable, biocompatible gold MPCs, templates for biomolecular functionalisation reactions. Biomolecular functionalisation strategies such as carbodiimide coupling, biotin-avidin interaction and …

    zulu Repository record

  5. Expression, Isolation and Purification of Human Zona Pellucida Protein 3

    Chi, Ting-Fung2019

    … vector, a transfecting-vector was constructed containing a 1.3 kb histidine tagged hZP3 cDNA. This histidine tagged hZP3 cDNA containing vector was transfected to human ovarian teratocarcinoma (PA-1) cells, and a single-cell clone producing the recombinant human ZP3 glycoprotein (rhZP3) was …

    odu Repository record

  6. The Expression of Chikungunya Virus Envelope 2 Glycoprotein Variants in Nicotiana benthamiana for the Development of a Diagnostic Reagent

    Naude, Jason Christopher DelvilleDepartment of Molecular and Cell Biology2020

    … worldwide. Furthermore, it has been recognised by the United States army as a potential biological weapon used for bioterrorism owing to the potential for infection via aerosol. CHIKV is primarily transmitted by infected Aedes aegypti mosquitos and is currently distributed in Africa, parts …

    cape-town Repository record

  7. Components of the Membrane Attack Complex (Mac) of Human Complement: Functional Studies of Their Macpf Domains

    Damour, Megan Spain

    … response that acts to</p> <p>eliminate pathogenic organisms. Complement is activated by three pathways leading to the sequential, non-enzymatic assembly of C5b, C6, C7, C8, and C9 to form the membrane attack complex (MAC). C6, C7, C8α, C8β, and C9 make up the “MAC family” of proteins. MAC …

    south-carolina Repository record

  8. Structure and Function of KH Domain in DDX43 and DDX53 Cancer Antigen Helicases

    Yadav, ManishaUniversity of Saskatchewan2020

    … to the DEAD-box RNA helicase family, and contain a K-homology (KH) domain in their N-terminal regions and a helicase core domain in their C-terminal regions. The KH domain was first characterized in the human heterogeneous nuclear ribonucleoprotein K (hnRNP K). The typical function of KH …

    sask Repository record

  9. Studies on the Function of Human Anti-HSP60 Monoclonal Antibody Fab

    장, 은정2011

    … 인자-alpha (TNF-a)와 인터루킨-6 (IL-6)와 같은 염증 매개체의 유도와 nitric oxide (NO)등의 방출을 증가시키며, Th-1 촉진 싸이토카인 인터루킨-12 (IL-12)와 인터루킨-15 (IL-15)의 유전자 발현을 유도한다. 이러한 염증 반응의 촉진을 통하여 열충격 단백질-60은 조직손상과 염증성 질환을 유도한다. 본 연구실에서는 선행 연구로 파아지 디스플레이 (phage display) 기법을 이용하여 인간 재조합 항-열충격 단백질-60에 대한 단클론 항체 Fab (HSP60-45)를 제작하였다. …

    ajou Repository record

  10. Molecular Characterization of pFGE, the Paralog of the C-α-Formylglycine-generating Enzyme

    Mariappan, Malaiyalam2012

    … pFGE-überexprimierender Fibroblasten über Ni-NTA-Agarose und Anionenaustauscher-Chromatographie gereinigt und monokloale Antikörper und polyklonale Antiseren für Western-Blot-Analysen hergestellt. Limitierte Proteolyse von gereinigtem FGE und pFGE an analogen Spaltstellen gab Hinweise auf …

    goettingen Repository record

  11. Mechanistic investigations of the radical transport pathway in fluorotyrosine-substituted class Ia ribonucleotide reductases

    Ravichandran, KanchanaMassachusetts Institute of Technology2016

    … la RNR that is composed of two homodimeric subunits ([alpha]a2 and [beta]2), which form an active [alpha]2[beta]2 complex. A stable diferric-tyrosyl radical (Y₁₂₂*) in [beta]2 reversibly oxidizes an active site cysteine (C₄₃₉*) in [alpha]2 via multiple proton-coupled electron transfer (PCET) …

    mit Repository record

  12. Alpha-Dioxygenase aus Erbsen - Studien zu Expression und Enzym-Substrat-Interaktion

    Meisner, Anke KarlaUniversität Würzburg

    … alpha-Dioxygenasen katalysieren in Pflanzen den Initialschritt der alpha-Oxidation von langkettigen Fettsäuren, die über die intermediäre Bildung von (R)-2-Hydroperoxyfettsäuren führt. Folgeprodukte dieser Reaktion sind die entsprechende (R)-2-Hydroxysäure sowie der um ein C-Atom kettenverkürzte …

    wurz-thes Repository record

  13. Expression, Reinigung und Charakterisierung von Makrolid-Glykosyltransferasen für die Synthese neuartiger Makrolid-Antibiotika

    Schumacher, ThomasPublikationsserver der RWTH Aachen University2005

    … and TylMII were produced by medium-scale fermentation of recombinant Saccharopolyspora erythraea strains. The soluble expression of all glycosyltransferase genes was verified by immunoblotting and ELISA against the C-terminal polyhistidine-tag of the enzymes. During a 30 L cultivation of …

    aachen Repository record

  14. Investigation of the role of the GGMP motif of Plasmodium falciparum Hsp70-1 on the chaperone function of the protein and its interaction with a co-chaperone, PfHop

    Makumire, Stanley2019

    … expresses an Hsp70 (PfHsp70-1) which plays a significant role in parasite survival. PfHsp70-1 is distinct in that it possesses glycine-glycine-methionine-proline (GGMP) tetrapeptide repeats in its C-terminal domain. To date, the GGMP motif of PfHsp70-1 has not been studied. The motif is …

    venda Repository record

  15. Novel Inhibitors for Isocitrate Lyase as a Potent Antitubercular Agent for Mycobacterium Tuberculosis

    Gaspar Quinonez, C.University of Westminster2021

    … using E. coli as a host and purified by Ni-NTA chromatography. The recombinant enzyme was used to set up crystallisation trials and in vitro enzymatic assay was performed on ICL1 inhibitors. The synthesis of D/L-threo-2-methylisocitrate and the methylation of five other drug candidates …

    westminster Repository record

  16. Identification of Novel Allosteric Regulators of Human Erythrocyte Pyruvate Kinase

    Kharalkar, Shilpa S.2014

    … kinase liver red blood cell) gene31, 32. Clinically, R-PK deficiency manifests itself as a chronic life-long hemolysis ranging from very mild or fully compensated anemia to life-threatening neonatal anemia and pronounced jaundice. Current treatment options are limited to continuous blood …

    vcu Repository record

  17. Röntgenstrukturanalyse der 6-Hydroxy-D-Nikotin-Oxidase aus Arthrobacter nicotinovorans sowie Darstellung und Charakterisierung einer Protein-Protein-Kontaktfläche mit der 6-Phospho-ß-Galaktosidase aus Lactococcus lactis

    Kötter, Jochen

    Die 6-Hydroxy-D-Nikotin-Oxidase (6HDNO) aus Arthrobacter nicotinovorans wurde rekombinant exprimiert, gereinigt und kristallisiert. Die Struktur wurde mit röntgenkristallographischen Methoden in drei Kristallformen bis zu einer Auflösung von 1.9 Å gelöst und verfeinert. Das Enzym katalysiert die …

    freiburg-diss Repository record

  18. Characterization of Cytotoxicity against Cancer Cells induced by Human Monoclonal Antibody Specific to TRAIL-R1

    이, 유리2011

    … are resistant to sTRAIL treatment. Recently, agonistic antibodies to TRAILR1(DR4) or TRAIL-R2(DR5) have been developed for alternative anti-cancer drug. Although they specifically bound their target receptors and induced apoptosis in a variety of cancer cells, most antibodies developed are mouse …

    ajou Repository record

  19. Chemically Specific Protein Immobilization Strategies on Analytical Surfaces

    Dembowy, Szymon2005

    … of proteins after immobilization process. Using nitrilotriacetic acid (NTA) linkers and polyhistidine-tagged proteins, several synthetic strategies for presenting these linkers to the surface are illustrated. Gold was used as a primary surface. Data was also obtained for other potentially useful …

    ncsu Repository record

  20. Functional stability of plant peroxiredoxins

    Jacob, SimoneUniversität Bielefeld

    … transcript regulation during different developmental states, in various organs and tissues as well as in dependence of a multitude of exogenous stimuli revealed differential regulation patterns for PrxII B and PrxII C/D. The impairment of functional stability, which was observed during …

    bielefeld Repository record

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