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Showing 1 to 6 of 6 for “"Nfa1 protein"”.
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Role of the Nfa1 Protein in Pathogenic Naegleria fowleri Co-cultured with Target Cells
… we previously cloned an antigenic gene (called nfa1) from a cDNA library of N. fowleri by immunoscreening. The nfa1 gene had the coding sequence of 360 bp, producing a 13.1kDa recombinant protein (rNfa1). Anti-Nfa1 polyclonal antibody, revealed pseudopodia-specific immunolocalization of Nfa1 …
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Protective Immunity in Mice Immunized with the Nfa1 Protein for Pathogenic Naegleria fowleri Infection
… study, to examine the protective immunity of the Nfa1 protein for N. fowleri infection in a mouse model, BALB/c mice were immunized with the Nfa1 protein either by intraperitoneal or intranasal route, and then infected intranasally with N. fowleri trophozoites. In regardless of the immunization …
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Production and Characterization of Monoclonal Antibodies against a Recombinant Nfa1 Protein Cloned from Pathogenic Naegleria fowleri
… antibodies against a recombinant His-tag fusion Nfa1 protein cloned from pathogenic N. fowleri were produced, and its characterization was elucidated. In addition, to observe the usage in immunological studies, the localization of the Nfa1 protein in N. fowleri was observed by immunofluorescent …
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Knockdown of nfa1 Gene Cloned from Naegleria fowleri by Antisense RNA
… adults. There have been few reports to address proteins functioning in vitro cytotoxicity of N. fowleri. The nfa1 gene, cloned from a cDNA library of N. fowleri by immunoscreening, should be concerned with the formation of food-cups that is a phagocytic structure. In addition, an anti-Nfa1 …
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Cytopathic Effects of a Pathogenic Naegleria fowleri and an Anti-Nfa1 Antibody on Rat Microglial Cells
… of amoeba to host cells is most important. The nfa1 gene (360 bp) was previously cloned from a cDNA library of pathogenic N. fowleri by immunoscreening, and produced a 13.1 kDa recombinant protein (rNfa1) that showed the pseudopodia specific localization by immunocytochemistry. On the basis of …
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The effect of nfa1 accination against Naeglearia fowleri infection
… in experimental animals and in humans. The nfa1 gene that is cloned from N. fowleri is located on pseudopodia, especially amoebic food-cups and plays an important role in the pathogenesis of N. fowleri. In this study, the retroviral vector (pQCXIN) and the lentiviral vector (pCDH) cloned …