Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 156 for “"Next generation sequencing (NGS)"”.
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Next-generation sequencing (NGS) for human disease research
차세대 염기 서열 분석법(Next-generation sequencing)은 DNA 유전 정보를 읽어내는 기술을 자동화한 1세대 생어(Sanger) 방식에서 대량의 병렬 데이터 생산이 가능하게 진화한 차세대 염기 서열 분석기가 생산한 데이터를 분석하는 기법을 의미한다. 2007년, 차세대 염기 서열 분석법의 등장은 무어의 법칙(Moore's law)을 능가하는 속도로 염기 서열 분석 비용을 감속시키고 있으며 곧 한 사람의 염기 서열 분석이 $1,000에 가능하게 되리라는 것이 명확해졌다. 차세대 염기 서열 분석법은 정착 단계를 …
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FPGA acceleration of short read alignment with high-level synthesis
With the introduction of next-generation sequencing (NGS) technologies, DNA sequencing is becoming an increasingly widespread process. When performed on human patients, it can allow for the prediction and prevention of diseases. An essential part of this bioinformatics pipeline is short read …
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Identification of endophytic bacterial communities associated with South African crops: sorghum bicolor (L. Moench), pennisetum glaucum and arachis villosulicarpa
… fragment length polymorphism (t- RFLP) and next generation sequencing (NGS). The first objective of this study was to investigate the effect of different DNA extraction protocols on mDNA yield and quality, as well as the diversity of endophytic bacteria retrieved from root and stem tissues …
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Next Generation Sequencing: Applications for the Clinic
… important for diagnosis of many diseases. Next Generation Sequencing (NGS), while commonly used as a research tool, is steadily making its way into clinical labs. One advantage of NGS is found in the observations that can be made, in addition to primary sequence, by analyzing raw data. This …
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DYNAMICS OF MICROCYSTIS-INFECTING CYANOPHAGES IN TROPICAL FRESHWATERS THROUGH QPCR AND NEXT GENERATION SEQUENCING
… Ma-LMM01-type cyanophages and (2) to use Next-Generation Sequencing (NGS) to further our understanding on the viral community and diversity. Results from this thesis study showed the influence of environmental variables (monsoon seasons) on predator-prey dynamics and leveraged on NGS to …
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A comparison of two methods of template amplification for next generation sequencing: Implications of polyclonal formation on DNA sequence for several cancer tissues
<p>Next-Generation Sequencing (NGS) technology has advanced the field of personalized medicine by predicting effective treatments for cancer patients using genomic sequence data, including the detection of oncogenes (genes involved in cancer development). Simultaneous amplification of multiple DNA …
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Scalable methods for storage, processing and analysis of sequencing datasets
Massive amounts of next-generation sequencing (NGS) reads generated from sequencing machines around the world have revolutionized biotechnology enabling wide-scale disease and variation studies, personalized medicine and helping us understand our evolutionary history. However, the amount of …
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Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device
… readouts for targeted downstream assays such as Next-generation sequencing (NGS) or improved quality of cells for biomanufacturing.
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Error Correction in Next Generation DNA Sequencing Data
Motivation: High throughput Next Generation Sequencing (NGS) technologies can sequence the genome of a species quickly and cheaply. Errors that are introduced by NGS technologies limit the full potential of the applications that rely on their data. Current techniques used to correct these errors …
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Bioinformatics and Next Generation Sequencing: Applications of Arthropod Genomes
Over the past decade, the Next Generation Sequencing (NGS) technology has been broadly applied in many areas such as genomics, medical diagnosis, biotechnology, virology, biological systematics, forensic biology, and anthropology. Taken together, it has offered us brilliant insights into life …
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Application of CRISPR/Cas9 to edit genes affecting seed morphology traits in wheat
… effective wheat genome editing pipeline. We used next-generation sequencing (NGS) data to estimate genome editing efficiency of many gRNAs using the wheat protoplast assay and choose the most efficient gRNAs for plant transformation. We successfully applied this pipeline to five wheat orthologs of …
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Development of Novel Methods to Minimize The Impact of Sequencing Errors In The Next-Generation Sequencing Data Analysis
<p>Next-generation sequencing (NGS) technology has become a prominent tool in biological and biomedical research. However, NGS data analysis, such as <em>de novo</em> assembly, mapping and variants detection is far from maturity, and the high sequencing error-rate is one of the major problems. …
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Mutational Analysis of HIV-1 Nucleocapsid Protein and Methods Development for Aptamer Discovery
… were set up to imitate the two main-stream next-generation sequencing (NGS) technologies, bridge amplification based and emulsion PCR based NGS technologies. Proof-of-principle experiments were carried out and optimized to evaluate the practicality of these platforms in high-throughput and …
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Developing Bottom-Up, Integrated Omics Methodologies for Big Data Biomarker Discovery
… computing compliments the proliferation of next generation sequencing (NGS) and genome-wide association studies (GWAS) datasets. These data sets are often complex, poorly annotated or require complex domain knowledge to sensibly manage. These novel datasets provide a rare, multi-dimensional …
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The impact of a variety of factors on the obesity associated gut microbiota
… over the last decade advances in the field of next generation sequencing (NGS) have facilitated investigations to determine the relationship between the gut microbiota and obesity. This thesis examines the impact of a variety of factors on the obesity associated gut microbiota. Overall the …
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Diagnosi molecolare della Distrofia Muscolare di Duchenne e della Neurofibromatosi tipo 1 con la tecnologia di sequenziamento Next Generation
… and complex, lacking mutational "hot spots". Next Generation Sequencing (NGS) technology is useful for molecular diagnosis of diseases where laborious sequencing efforts are required. The aim of this study was to work out a method to use the high-‐throughput NGS technology in a diagnostic …
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Biomarkers of Human Exposure and Effect to Petrogenic Polycyclic Aromatic Hydrocarbons
… of petrogenic PAH exposure. Additionally, Next Generation Sequencing (NGS) and quantitative Real Time – Polymerase Chain Reaction (qRT-PCR) were used to identify and verify differentially expressed circulating plasma microRNAs that may serve as novel minimally invasive biomarkers of effect …
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The use of environmental DNA for the detection of Palaemonias ganteri (Hay, 1901), a federally endangered cave species
… techniques. Advancements in metabarcoding and Next Generation Sequencing (NGS) technologies provide tools that may allow researchers and managers to address some of the hurdles posed by the difficult environment where P. ganteri lives. </p> <p>This study was able to identify the presence of P. …
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Compressive algorithms for search and storage in biological data
… in biological data can be leveraged also for next-generation sequencing (NGS) read mapping. By pairing together a clustered grouping over similar reads and a homology table for similarities in the human genome, our CORA framework can accelerate all-mapping by several orders of magnitude. …
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