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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Neutravidin"”.
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Ortsaufgelöster Aufbau von DNA-Nanostrukturen auf Glasoberflächen
… Funktionsmolekülen nutzt das Bindungspaar Biotin-Neutravidin. Mit Hilfe eines Rasterkraftmikroskops (AFM) wurde eine zu einem „Stift“ umfunktionierte Rasterkraftmikroskopspitze so mit der zu deponierenden „Tinte“ beladen, dass das Absetzen von Neutravidin im unteren Mikrometermaßstab möglich war. …
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Development of Protein Based PCBP2 siRNA Nanocomplex for Liver Fibrosis Therapy
… different variants of avidin such as avidin, neutravidin and streptavidin for in vitro activity and cellular uptake over the extended time interval. Addition of polyethylene glycol (PEG) spacer between the biotin and cholesterol ligand was done to improve the biodistribution of the …
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Analyse von Single Nucleotide Polymorphisms an Glas-Oberflächen
… PCR-Produkten an Streptavidin-, Avidin- und NeutrAvidin-Oberflächen sowie die kovalente Bindung von phosphorylierten Amplifikaten mit der EDC/Methylimidazol-Methode. Die besten Ergebnisse sowohl in Spotform und -homogenität als auch im Signal/Rausch-Verhältnis wurden an …
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Discovery of PSMA-Specific Peptide Ligands and Development of A Noncovalent Method to Attach Chemical Moieties to siRNA
… which was then mixed with biotin-peptide-431 and neutravidin to formulate neutravidin-based nanocomplex. This nanocomplex exhibits excellent silencing activity in vitro.
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Design and development of a site specific protein patterning technique for use in a microfluidic antibody separation device
… conjugation of streptavidin, streptavidin-FITC, NeutrAvidin, single domain protein L and multidomain protein L to silicon surfaces, upon reaction with UANHS, were investigated. Fluorescently labelled probes that associated with the protein motifs were used to confirm successful conjugation of …
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Charge-based transport and drug delivery into cartilage for localized treatment of degenerative joint diseases
… than its electrically neutral counterpart (Neutravidin), and remained bound within cartilage for at least 15 days. Competitive binding experiments revealed that despite Avidin's weak and reversible ionic binding (dissociation constant, KD ~150 [mu]M) to the negatively charged …
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Switchable biological surfaces
… that less binding took place between the neutravidin and the biotinylated peptide under a negative potential due to decreased image intensity. Control experiments were also carried out using non-biotinylated peptides to show there was minimal non-specific binding and that binding was only …
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Development of novel diagnostics and therapeutics for amyotrophic lateral sclerosis
… binding proteins (avidin, streptavidin, and neutravidin) to specifically conjugate TTC to the nanoparticle surface. TTC nanoparticles were shown to selectively target neurons and not other cell types in vitro.
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Controlling Surface Chemistry on the Microscopic and Nanoscopic Scale through Photopatterned Self-Assembled Monolayers
… intensity during patterning. Moreover, adsorbed neutravidin was detected using a</p><p>commercial biotin labeled anti-avidin antibody, which allowed for significant signal enhancement over background. The ability to produce complex protein patterns will contribute greatly to creating in vitro …
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Hydrodynamic and Mass Transport Properties of Microfluidic Geometries
… limit, validating the results for the prototype NeutrAvidin-biotin assay. The second part of this work discusses the dielectrophoretic motion of surfactant-stabilized water droplet pairs in a microchannel as they approach and coalesce under a uniform electric field. Experimental data measuring …
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An Examination Of The Cytoplasmic Dynein Stepping Mechanism At The Single Molecule Level
… and coat them with the biotin binding protein, NeutrAvidin, in order to attach them to biotinylation sites within the dynein ring. A method was also developed to quantify the number and density of functional biotin binding sites on the nanorod surface and compare it to that of commercially …
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Development of Targeted siRNA Therapeutics for Triple Negative Breast Cancer and Liver Fibrosis
… chemotherapy. In Chapter 5, we prepared three neutravidin-based siRNA nanocomplexes with different targeting ligands to deliver PCBP2 siRNA to hepatic stellate cells (HSCs). Insulin-like growth factor 2 receptor (IGF2R) is overexpressed in activated HSCs and therefore can be utilized for …
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Point-of-care microfluidic assays for measuring expression level of antigen on blood cells
… in a capture chamber that is functionalized with neutravidin. Beads that have a higher density of travel, on average, shorter distances in the chamber before they get captured compared to the beads with a lower density of biotin. We developed a statistical model to extract the probability of …
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Photopolymerization-based signal amplification : mechanistic characterization and practical implementation
… limits were encountered coupling eosin and neutravidin to a poly (acrylic acid-co-acrylamide) backbone, we synthesized a more water-soluble polymeric macrophotoinitiator based on a generation 7 poly (amidoamine) dendrimer scaffold. Although the solubility was improved, a new quenching …
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Herstellung und Untersuchungen von polymerbasierten Wirkstofftransportsystemen
… durch die Bindung von Fluorescein-markiertem Neutravidin am Polyester-Blockcopolymere direkt nachgewiesen werden. Vor allem die Anbindung von Kohlenhydraten, wie Glucose, Galactose und Nona-Zucker, konnte erfolgreich gezeigt werden. Dazu wurden die Kohlenhydrate an der α-Position der …
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Generation and application of recombinant antibody fragments for prostate cancer detection.
… anti-fPSA biotinylated scAb was captured on a neutravidin-coated ELISA plate and fPSA sandwiched using the avian antibody fragments as the detection antibodies. The assay sensitivity was improved using this format with a limit of detection of 1.8 ng/mL in PBST solution. Female serum was spiked …