Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 29 for “"Neutralising antibodies"”.
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The screening of neutralising antibodies against a resistant HIV-1 strain to identify novel epitopes
… cure. However, producing immunogens that elicit neutralising antibodies that are protective against HIV-1 acquisition has proven difficult. This is not only because of the genetic diversity of the viruses circulating in the human population but is also as a result of an incomplete understanding …
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Role of envelope compactness and glycosylation in HIV-1 resistance to neutralising antibody responses
… isolates that are highly resistant to broadly neutralising antibodies could limit the efficacy of an antibody-based vaccine. For this reason, it is important to understand the mechanisms behind high HIV-1 resistance to neutralising antibodies. Chapter 2 and Chapter 3 of this thesis describe …
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In vitro selection and characterisation of human anti-HIV-1 antibody fragments
Generation of neutralising antibodies with broad specificity would be one of the effective approaches to control HIV-1 spread. It is clear that a method that allows rapid generation of neutralising antibodies is needed. This project aims at developing a novel approach to rapidly access human …
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Secretion of the rotavirus VP7 capsid glycoprotein from polarised epithelial cells
… layer of the virion where it is a key target for neutralising antibodies, which act to prevent virion decapsidation. Both polarised and non-polarised cell culture models have been widely used in rotavirus research. However, cell type-dependent differences in rotavirus release and viral protein …
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Site-Specific Labelling of the Flavivirus Envelope Protein using Genetic Code Expansion
… entry into cells and is the primary target of neutralising antibodies. The E proteins arranged on the surface of the virion are not static and pose a dynamic landscape for recognition by host factors, including antibodies. Many antibodies bind virion surfaces not predicted to be accessible …
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Design of novel delivery systems to probe alternative routes of administration for a self-amplifying RNA rabies vaccine
… there is an established correlate of protection (neutralising antibodies) and there exist efficacious vaccines in the market (e.g. Rabipur) to be used as comparators. To this end, a SAM vaccine encoding the rabies virus glycoprotein (RVG), the only target for neutralising antibodies, was …
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The role of scavenger receptor B-I in hepatitis C virus attachment and entry.
… lipoproteins and a heightened sensitivity to neutralising antibodies.
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Immune responses to AAV gene therapy in the ocular compartment
… into the role that pre-existing and induced neutralising antibody responses may play in the context of ocular gene therapy. This concluded that a multifaceted/comprehensive approach may be required to circumvent anti-capsid humoral immunity and enable repeated gene transfer in humans. As …
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Targeted expression of the anti-HIV microbicide lectin griffithsin in maize and tobacco
… al., 2003). Protein based microbicides,-namely, neutralising antibodies and peptide lectins- lend themselves to production in plants (De Muynck et al., 2010; Matoba et al., 2010; Sexton et al. 2006; O’Keefe et al., 2009). One of these lectins, namely Griffithsin (GRFT) was isolated from the blue …
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Investigation of particulate HIV-1 Env vaccine candidates using Zera® and SpyTag/SpyCatcher technologies
… could improve the induction of long-lasting neutralising antibodies (NAbs). For this thesis, two different nanoparticle approaches were therefore investigated. The HIV-1 Env sequence used for both approaches was derived from the superinfecting subtype C CAP256 virus. This was truncated to …
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An investigation of the regulation of Sialoadhesin ligands on CD4+ T cells and its potential significance in Systemic Lupus Erythematosus
… study the role of Sn in this model. First, Sn neutralising antibodies were used prophylactically to study the effect on disease severity. Secondly, using speed congenic method, BWF1 mice deficient for Sn were generated. Data presented in this thesis suggest that Sn might play an …
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Evaluation of a Tetravalent DNA Vaccine against Dengue: Integrating Biochemical Studies on Dengue Virus Envelope Protein to a Domain-Based Antigen Design.
… (E) is the main target of neutralizing antibodies, it has been used as the antigen of choice for vaccine development efforts. Here we present a detailed analysis of factors involved in the expression, secretion and folding of the E ectodomain from all four DENV serotypes and ZIKV in …
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Characterisation of llama antibody fragments able to act as HIV-1 entry inhibitors
… fusion of viral and cellular membranes. Neutralising antibodies inhibit HIV-1 entry by targeting epitopes on the functional spike. HIV-1 has, however, evolved several ways to evade recognition by antibodies, including variable regions, carbohydrates, and conformational masking. As a …
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The role of virus neutralisation in immunity to feline immunodeficiency virus infection
… of HIV. It is accepted that the induction of neutralising antibodies (NAbs) is a key element in the control of lentiviral infection, since T-cell based vaccines alone failed to prevent infection in most experimental animal model systems. In this project a robust and reproducible in vitro …
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Exploration of novel activin A antibodies
… activation, I characterised novel α-activin A antibodies with biophysical and structural techniques. These binders included the dual-specific B52 antibody, which targets the growth factor domain of activin A and closely-related isoform activin B, in addition to eight non-neutralising antibodies …
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Identification of novel regulators of lymphocyte development and differentiation using CRISPR-Cas9 screens
… and ZIP7. Notably, small molecule inhibitors and neutralising antibodies confirmed a previously unappreciated, but critical role of αv, in combination with integrin β3, in TH2 cell differentiation. The generation of mice with a conditional deletion of αv in T cells corroborated this essential role …
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Computational Methods to Design Broad-Spectrum Medical Countermeasures Against Antigenically Diverse Pathogens
… are molecules recognised by T-cells and antibodies of the immune system. This antigenic diversity can reduce the effectiveness of existing vaccines and treatments. This dissertation explores how computational approaches, such as graph-based and deep learning methods, can be used to design …
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An investigation into the characteristics and potential therapeutic application of human neural progenitor cell-derived astrocytes in experimental spinal cord injury
… novel medications (including the use of blocking/neutralising antibodies and peptides), cell-based intervention strategies (including stem/progenitor cells) or, most recently, the application of tissue engineering strategies using biomaterials. The aim of the present study was based on the two …
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Neutralising antibody responses in acute hepatitis C and SARS-CoV-2 infection
Neutralising antibodies (nAbs) are capable of blocking viral entry to host cells. Understanding the nAb response in early infection informs preventative and therapeutic interventions. The acute phase of hepatitis C virus (HCV) infection is characterised by two bottlenecks of viral diversity, one at …
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Characterisation of Novel Secreted Antiviral Factors to Human Cytomegalovirus
… antiviral restriction factors, NK cells, neutralising antibodies and polyfunctional CD4+ and CD8+ T-cells. Although critical roles have been established for the antiviral cytokines IFNγ and TNFα during HCMV infection, the contribution of soluble factors to viral control remains poorly …
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