Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"Neutralisation assay"”.
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The role of crude saliva and purified salivary mucins in the inhibition of the Human Immunodeficiency Virus type 1
… mucin components inhibited HIV-1 in an in vitro assay. Mucins from infected saliva did not show inhibition in this assay. Mucus was extracted in 6M guanidinium hydrochloride and a cocktail of protease inhibitors, pH 6.5. Sepharose CL-4B gel filtration separated MUC5B and MUC7 in saliva. Mucins …
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The study of highly pathogenic emerging zoonotic virus envelope proteins through pseudotyped virus generation
… virus) isolate. Screening polyclonal sera via a neutralisation assay, the immunologically dominant sites for phylogroup I and III were identified as III and I respectively. This can act to inform future development of more broadly neutralising vaccines. The 2013-16 outbreak of Ebola virus focused …
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The role of virus neutralisation in immunity to feline immunodeficiency virus infection
… this project a robust and reproducible in vitro neutralisation assay was developed and optimised, permitting the assessment of the NAb response in naturally infected cats and with the potential to evaluate candidate vaccines. It was demonstrated that, in general, primary FIV strains in the UK …
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HPV pseudovirion production in plants
… of a robust and sensitive pseudovirion-based neutralisation assay (PBNA), which has become the gold standard neutralisation assay for the testing of candidate HPV vaccines. The currently accepted PsV production method utilises mammalian cell culture to produce HPV PsVs, encapsidating a SEAP …
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The effect of reduction, trypsin digestion and de-glycosylation of salivary mucins in the inhibition of human immunodeficiency virus type 1
… were tested for cell cytotoxicity using an MTT assay. This was then followed by a neutralisation assay which uses HIV-1 env pseudo virus (DU422.1 and YU2 subtype C and subtype B respectively) and a luciferase reporter gene involving modified TZM-bl/JC cells was used to test the inhibitory …
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Neutralising antibody responses in acute hepatitis C and SARS-CoV-2 infection
… clearers were also more susceptible to neutralisation compared to chronic progressors. The second part of this thesis describes how the techniques utilised to study nAb responses in HCV were adapted to study Severe acute respiratory syndrome (SARS) coronavirus (CoV) -2, hoping to unravel …