Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"Neurospheres"”.
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Isolation, Culture and Cryopreservaion of Neural Stem Cell
… Both GFP (+) and (-) cells could generate neurospheres but the differentiation potential into oligodendrocyte was reduced in eGFP(-) neurospheres. To determine differentiation ability neurosphere from single cell, we selected by clonal selection and cultured for 10 day. Single cell was …
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Characterisation of neural progenitors from the adult retina and ciliary epithelium
… retina are proliferative and give rise to neurospheres in vitro, a characteristic of neural stem cells. However, these adult-derived CNR progenitors differ from those of the wildtype CE, leading to de-pigmented, larger and more numerous neurospheres expressing Müller glial cell markers. My …
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Uncovering the role of notch signaling in development of hypothalamic nuclei in vivo and using in vitro microenvironments
… (HVZ). These cells expand and spontaneously form neurospheres which can be manipulated and studied at the level of mRNA or protein expression. We found that acute chemical inhibition of Notch signaling was sufficient to significantly reduce direct downstream Notch signaling Hes1, Hes5, and Hey1. …
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Bioengineering of a TAT-conjugated Peptide to Modulate the Activity of Glycogen Synthase Kinase-3 in Adult and Embryonic Stem Cells
… significant positive effect on the formation of neurospheres in embryonic stem cell cultures and on the survival of myeloid progenitors in cytokine-starved fetal liver cell cultures. On the other hand, GSK-3 inhibition reduced the number of neurospheres generated by human olfactory …
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EXAMINATION OF CD133 AND CD147 AS CANCER STEM CELL MARKERS
… we found evidence which shows that formation of neurospheres with U87MG glioblastoma cells may be driven by increased expression of CD147, correlated with increased CD133 expression. These findings suggest that relative levels of CD147 expression may be used as a determinant to target cancer stem …
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Involvement of CD133+ stem cells in the chemoresistance of glioma
… medium (SFM). In SFM, the U87MG cells grew as neurospheres suggesting the presence of stem cells in this cell line while in the 1321N1 cells no neurospheres were observed. Immunocytochemistry and fluorescence-activated cell sorting (FACS) analysis revealed the absence of CD133+ cells in 1321N1 …
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Targeting DNA Double-Strand Break Repair to Potentiate Radio- and Chemo Therapy of Glioblastoma
… tumors (generated by xenografting GBM9 neurospheres) leads to acquired-TMZ resistance resulting in tumor recurrence. In order to understand the basis for therapy-driven TMZ resistance, we generated and functionally characterized ex-vivo cultures from the primary and recurrent tumors. We …
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P107 negatively regulates the neural precursor pool by repressing Hes1 transcription
… we showed that removal of Hes1 from p107-/- neurospheres restores the number of neurosphere forming cells to wildtype levels. Our results suggest that p107 represses Hes1 transcription through E2F, and demonstrate that an upregulation of Hes1 is responsible for the increased neural precursor …
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Bioengineering Microporous Annealed Particle Scaffolds to Recruit Neural Progenitor Stem Cells and Promote Angiogenesis in the Stroke Core
… through maintained stemness and the formation of neurospheres or towards Tuj1 positive NPCs, as well as enhance angiogenesis and the recruitment of endogenous NPCs after stroke. We have observed the first case of significant angiogenesis throughout the entire stroke core within only 10 days after …
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The role of the dopaminergic neurotrophin growth/differentiation factor-5 in the developing rat ventral midbrain
… on the proportion of cell types generated from neurospheres from E12, E13 and E14 VM was examined. Dopaminergic neurons could only be generated from neurospheres which were prepared from E12 VM. Thus in subsequent studies the effect of GDF-5 on dopaminergic induction was examined in progentior …
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Novel Regulation of the Cell Cycle During Cell Fate Decisions in the Central Nervous System
… parameters including the number and longevity of neurospheres. These data demonstrate for the first time that the Spy1/RINGO family plays an important role in neural fate decisions and that overexpression of Spy1 is a driving factor in specific forms of neural cancer.
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Fas-associated factor 1 is involved in neurofibrillary tangle-mediated cell death of basal forebrain cholinergic neurons in P301L transgenic mice
… of recombinant Reelin 77 3. Culture of neurospheres 78 4. Immunofluorescence 79 Ⅲ. RESULTS 81 Ⅳ. DISCUSSION 99 Ⅴ. CONCLUSION 103 REFERENCES 104 국문요약 116
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Neural Stem / Progenitor Cells in the Adult Mouse Hypothalamus
… the neurosphere assay. I show that hypothalamic neurospheres can be propagated in culture for extended periods of time, and that they can differentiate into cells of all three neural lineages. I have also determined the precise location of neurosphere-forming cells in the hypothalamus, showing …
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The Role of Notch1 in Adult Hippocampal Neurogenesis and Function
… Notch1 iKO mice were incapable of forming new neurospheres with extended passaging. While non-running Notch1 iKO mice had fewer YFP+ SGZ cells relative to wild type littermates, Notch1 iKO mice given 30 days access to a running wheel had equal number of YFP+ SGZ cells relative to controls, …
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The Role of Glutamine and Mevalonate Pathway In Affecting The Stemness and Viability of Glioblastoma Stem Cells
… cultured in serum-free medium form self-renewing neurospheres, express the neural stem marker CD133, and are highly tumorigenic. On the other hand, in the presence of fetal bovine serum (FBS), the glioblastoma stem cells undergo differentiation.</p> <p>In this study we used glioblastoma stem …
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The neuronal RNA binding protein HuB as a potential tumor suppressor in glioblastoma
… migration, invasivity and the capacity to form neurospheres, supporting the hypothesis that HuB act as a tumor suppressor. We finally showed that HuB is able to determine an increase of p21 protein in normal murine neuroblast cells, providing a possible mechanism for HuB-mediated suppression of …
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Aligned Fibrillar Collagen Matrices for Tissue Engineering
… viability and adherence of spinal cord-derived neurospheres. Together, these results demonstrate the feasibility of aligned collagen matrices as a versatile platform to control cell behaviour towards tissue regeneration. Ultimately, the new method to align collagen fibrils and to coat hollow …
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Myxoma Virus Therapy of Malignant Gliomas in Murine Syngeneic Models: Translating Barriers to Therapy
… culturing spontaneous gliomas from these mice as neurospheres. These lines displayed stem cell markers and produce highly invasive gliomas when grafted into the brains of immunocompetent C57Bl/6 mice. We hypothesized that Type-I interferon (IFNα/β), could be playing a role in this resistance. …
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HIV-1 INFECTION OF NEURAL STEM CELLS RESULTS IN COGNITIVE DEFICITS THROUGH ADULT NEUROGENIC MODULATION
… mouse NSCs were unable to form as many primary neurospheres as WT NSCs. Additionally, when the NSCs were stratified by size, Tg26 NSCs formed lower numbers of smaller-sized primary neurospheres and more larger-sized primary neurospheres. These findings demonstrated that low-level chronic HIV-1 …
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