Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"Netrin"”.
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Molecular biology of peripheral nerve regeneration: Expression and role of Netrin-1, DCC and Runx2
… and role of an axon guidance molecule - Netrin-1 in peripheral nerve repair. Using a Schwann cell-specific Netrin-1 knockout mouse model, I showed an increased disorganisation and misdirection of re-growing axons within the nerve bridge following transection injury. However, this did not …
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Netrin Guides Spinal Commissural Axons to the Midline at Long Range Through the Receptors Dcc and Neogenin
… in a remarkably precise and stereotyped way. Netrin, a secreted laminin-related protein, provides the major attractive cue for midline guidance. It is thought to act at long-range, functioning either in solution (chemotaxis) or bound to surfaces (haptotaxis). A gradient of Netrin-1 along the …
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LATE PROTECTIVE EFFECTS OF NETRIN-1 AND ADENOSINE A2B RECEPTOR ACTIVATION IN THE MURINE ACETAMINOPHEN HEPATOTOXICITY MODEL
… options are needed. The neuronal guidance cue netrin-1 has been shown to promote hepatic repair and regeneration during liver ischemia/reperfusion injury, but its effects in APAP hepatotoxicity is unknown. The primary objective of this dissertation was to explore the role of netrin-1 and its …
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Signal Transduction Mechanisms in Commissural Axon Guidance: The Role of Intracellular Tyrosine Kinases in Netrin-Dcc/Frazzled Axon Attraction
… response to the the conserved axon guidance cue, Netrin. Netrin elicits midline axon attraction in all bilaterally symmetric animals studied to date through the DCC family of receptors. In Drosophila, commissural neurons require Netrin and its DCC receptor, Frazzled, for midline axon crossing. To …
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Sema-1a Reverse Signaling Promotes Midline Crossing In Response To Secreted Semaphorins
… signals chemoattraction upon binding its ligand, Netrin. DCC mutations in humans are associated with mirror movement disorder, an inability to independently control the right and left sides of the body. Although Frazzled/Netrin signaling is required for many axons to cross the midline, netrin and …
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The role of Ena/VASP and associated proteins in regulation of neuronal morphology and filopodia architecture
… dynamics in response to the axon guidance cue Netrin-1 and its receptor DCC. I have shown Trim9 binds the cytoplasmic tail of DCC and also binds Ena/VASP proteins and Myosin-X, which are cytoskeletal effectors downstream of Netrin-1. I discovered that inhibition of Trim9 ubiquitin ligase …
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The cue induced axonal nascent proteome and its translational control mechanisms in neural wiring
… disease. Axons stimulated by different cues (Netrin-1, BDNF, Sema3A) each show a signature set of up/down newly synthesised protein (NSP) changes (>100) within 5 min. Remarkably, conversion of Netrin-1-induced responses from repulsion to attraction triggers opposite translational regulation …
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Axonal Translation and Links to Neuropathies
… multiple imaging techniques to investigate Netrin-1-induced translation in cultured retinal axons. Total axonal protein synthesis measured by metabolic and puromycin labelling indicates axons experience stage-dependent alterations in translation rate upon Netrin-1 stimulation. Remarkably, …
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The Evolution of Organ Shape : Distal Tip Cell Migration and Gonadogenesis in Nematodes
… migration. Here, we show early data that the Netrin cell guidance cue may be involved in dorsal/ventral migrations of the P. pacificus DTC. Specifically, we show Ppa-unc-40/Netrin receptor may be a major player in dorsal/ventral migrations of the DTC.
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Endogenous Mechanisms Regulating Myeloid Cell Mediated Inflammation During Acute Lung Injuries
… demonstrate that the neuronal guidance protein, Netrin-1, is highly expressed in infiltrating alveolar macrophages after the onset of ALI. Netrin-1 deletion in the myeloid compartment resulted in exacerbated ALI and increased NK cell recruitment, which was dependent on the upregulation of the …
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Growth Cone Pathfinding and Neuromuscular Synaptogenesis in Drosophila Midline Mutants
… I examined the function of commissureless, netrin, roundabout, and frazzled during neural development. Analysis of the individual axon pathways of RP3, V, and RP2 in wildtype and commissureless and roundabout mutants investigated how abnormal midline decisions affect subsequent growth cone …
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Methods to induce polarity in constructed neuronal networks
… protein patterns under visual control. Netrin-1, laminin-1 and ECM-gel were chosen as axon-guiding proteins, while poly-D-lysine (PDL) was printed as neutral protein. The protein transfer during 2-step µCP and the effect of the second printed pattern on the primary ones was characterized …
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The Cloning and Characterization of MADD-4, A Novel Guidance Cue in C. elegans
… arm extension, we found that the UNC-40/DCC netrin guidance receptor directs muscle arm extension to the midlines. Surprisingly, neither the UNC-6/netrin cue (the canonical ligand for UNC-40) nor other well-characterized guidance cues such as the slits, ephrins and semaphorins were found to …
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A lentiviral system for RNAi transgenesis and the Ena/VASP triple-knockout defines neuronal and non-neuronal functions in mouse development
… example, the Drosophila axon guidance ligands, Netrin and Slit, have recently been implicated in the development of several mammalian organ systems. While the characterization of extra-neuronal functions of ligands and receptors has emerged, the conservation of intracellular signaling pathways …
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The Intracellular Domain of the Frazzled/Dcc Receptor is a Transcription Factor Required for Commissural Axon Guidance
… through which DCC transduces signals from its Netrin ligands into local membrane and cytoskeletal rearrangements. In Chapter 2, I present data supporting the idea that the Fra ICD functions as a transcription factor to regulate axonal responsiveness to Slit-Robo repulsion at the midline of the …
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Dissecting the Development and Function of C. elegans Glia with Mutations of the mls-2 and vab-3 Genes
… are mediated, at least in part, by the UNC-6/Netrin protein. During the course of this study, I also confirmed that C. elegans CEPsh glia are not required for neuronal survival. Overall, the conservation of molecular features between the development of C. elegans CEPsh glia and vertebrate …
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The Guidance of Olfactory Sensory Axons to Identifiable Protoglomeruli in the Larval Zebrafish Olfactory Bulb
… I show for the first time in any system that netrin/DCC signaling is required to guide olfactory sensory axons to a specific location within the olfactory bulb. Interestingly, I find that the central zone protoglomerulus is innervated by neurons expressing related odorant receptors of the …
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Dscam Switches Slit Repulsion To Mild Attraction Via Robo Receptors
… via different axon guidance cues, such as Netrins and Slits. The axonalgrowth cone contains receptors that distinguish these guidance molecules andtranslates them into attractive or repulsive responses. Slit repels axons from theCNS midline by binding to the Robo (Roundabout) receptor. …
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