Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 52 for “"Nestin"”.
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Characterization of Neural Stem Cells Using the Nestin Enhancer
Nestin, one of the class VI intermediate filament, is known as a specific marker for neural stem cells and regulated by the enhancer located on the second intron of the gene. The second intron is highly conserved among species and crucial for the nestin expression in central nerve system (CNS) …
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Assembly and localization of the intermediate filament protein, nestin
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1995.
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Transcriptional regulators of nestin in the rat central nervous system
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1996.
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Evaluation of Sex Differences in the Hippocampus and Pituitary of Egr1 conditional knockout mice mediated by Nestin-Cre
… in neural cell lineage, we are using an Egr1cKO Nestin-Cre model. Nestin allows for targeting neuronal lineage specific cells. In Chapter 1, we provide a systemic view of Egr1 gene and Nestin-Cre as a system for generating conditional knockout mouse strains. The Chapter begins with the …
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Fattori prognostici nel melanoma cutaneo e nel tumore mammario associati a caratteristiche molecolari simil-staminali
Nestin (a neuronal stem cell/progenitor cell marker of central nervous system development), vimentin (which is ubiquitously expressed in mesenchymal cells), and the glucocorticoid receptor (GR, which is involved in the immune response, cell proliferation, and apoptosis) have been shown to interact …
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Identification, regulation and lineage tracing of embryonic olfactory progenitors
… of a select group of antigens, including nestin. Here, I show that the embryonic OE contains a novel radial glial-like progenitor (RGLP) that is not detected in adult OE. RGLPs express the radial glial antigens nestin, GLAST and RC2, but not brain lipid binding protein (BLBP), which, …
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Isolation, Culture and Cryopreservaion of Neural Stem Cell
Nestin is a member of the class Ⅵ intermediated filament proteins and a well-known stem cell marker in the neural lineage. The nestin gene consists of four exons and three introns. It has been known that the nestin transgene containing the second intron is sufficient to direct tissue specific …
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Neural stem cell differentiation in collagen scaffolds for retinal tissue engineering
… on neural differentiation and neurite growth. Nestin, [beta]III-tubulin, and GFAP expression were determined using immunofluorescent techniques, and the neurite length was measured. NSCs differentiated into neurons, with actively growing neurites, and astrocytes when cultured in differentiation …
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The effect of brain-specific VEGF-C deletion on vascular morphology in the brain and skull
… the role of VEGF-C in the brain, we generated Nestin-Cre; Vegfcfl/fl mice, in which we confirmed deletion of Vegfc. Nestin-Cre is expressed in neuronal and glial cell precursors, in which it mediates Cre-lox recombination of the floxed Vegfc allele. The objective of my study was to see if …
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Role of Sonic Hedgehog in Development of the Central Nervous System
… the effects of Shh on inducing the expression of nestin, the marker for neural stem/progenitor cells. In early stages, we found that Shh promoted the CNS-specific enhancer of the nestin gene through regulation of E-box on nestin 2nd intron 5’-region by Nmyc that is a target gene of Shh signaling. …
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The impact of diet-induced obesity and exercise on adipose-resident stem cells
… (NG2), including two pericyte subtypes based on Nestin expression (+/-). Male and female NG2-DsRed x Nestin-GFP reporter mice were placed on control or HFD for 6 wk prior to and following initiation of a 12 wk treadmill exercise program. NG2+Nestin- (Type 1 pericyte) and NG2+Nestin+ (Type 2 …
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The Role of Notch1 in Adult Hippocampal Neurogenesis and Function
… exercise-induced SGZ neurogenesis, we generated Nestin-creERT2/R26R-YFP/Notch1loxP/loxP (Notch1 iKO) mice to inducibly ablate Notch1 in Nestin-expressing stem and progenitor SGZ cells. The total number of YFP+ SGZ cells increased over time in wild type littermates, but not in Notch1 iKO mice. …
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Developmental Regulation of Prion Expression in Cattle and Mouse Embryonic Stem Cells
… resulted in an earlier up-regulation of PrPC and nestin (day 12 vs. day 16; P < 0.05). In addition, immunofluorescence studies indicated co-expression of PrPC and nestin in the same cells. The results of these experiments suggested a temporal link between PrPC expression and expression of nestin, …
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Expression profile of multidrug resistance genes and proteins in cancerous and stem cells
… major obstacle in treating gliomas. CD133 and nestin have been suggested as the markers of CSCs and natural stem cells. The primary focus of this study was to identify CD133+/nestin+ stem-like cells and discover their association with multidrug resistance (MDR) related genes, i.e. multiple drug …
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PERICYTE SUBTYPES AT THE INTERSECTION BETWEEN TISSUE REGENERATION AND PATHOLOGY
… subpopulations based on molecular markers, Nestin-GFP-/NG2-DsRed+/ PDGFRâ+ (type-1) and Nestin-GFP+/NG2-DsRed+/ PDGFRâ+ (type-2). This thesis characterizes heretofore their unknown specific roles. Only type-2 pericytes have the potential, under optimized culture conditions, to generate …
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Mash1 Defines Lineage Restricted Neuronal and Oligodendrocytic Precursor Cells in Spinal Cord Development
… over both gray and white matter. As a control, Nestin- CreERT2;R26R-lacZ and Nestin-CreERT2;R26R-YFP mice were used to confirm that common progenitors of all neural cell types can be traced from E10.5 to P21. This data provides evidence that Mash1 defines lineage restricted precursors that exit …
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Malignant Gliomas Originate From Neural Stem/Progenitor Cells and Are Maintained By Cancer Stem Cells
… we developed an NSC-specific tamoxifen-inducible nestin-cre driver mouse. When crossed to the NF/p53/Pten flox mice and induced with tamoxifen at E13.5, the Nes-Cre;Nf/p53/Pten mutant mice exhibited tumor initiation and progression similar to our previous mouse model using hGFAP-cre, with complete …
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Activation of Early Neural Progenitors Is Required for Traumatic Brain Injury-Induced Hippocampal Neurogenesis
… rtTA-IRES-eGFP expression under the control of a nestin promoter and also contains a neural progenitor-specific regulatory element. By using this line, I have demonstrated that eGFP-expressing cells represent early neural progenitors in the adult dentate gyrus. Performing unilateral controlled …
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THE VASCULAR RESPONSE WITHIN THE ISLET DURING BETA CELL DEVELOPMENT AND REGENERATION
… vasculature around weaning which may involve nestin+ cells. Destruction of β cells resulted in a disruption of the islet microvasculature. Re-organization of both tissue compartments appears to be co-ordinated around weaning in normal islet development and following β cell loss.
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An injectable, biomaterial-based therapy to promote endogenous neural progenitor cells in a hemorrhagic stroke lesion
… (EGF) to increase the number of endogenous nestin-positive neural progenitor cells (NPCs) in the lesion. In a well-validated intracerebral hemorrhagic (ICH) stroke model in rats, NPCs were quantified and compared to ICH-only controls at 2 and 4 weeks post-injection.
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