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Showing 1 to 20 of 53 for “"Nested PCR"”.

  1. Identificação das fases latente e replicativa do vírus Epstein-Barr: análise em tecidos parafinados de lesões de cabeça e pescoço através das técnicas de imuno-histoquímica, hibridização in situ e Nested PCR

    Rocha, Mônica Lage da2022

    … pelo vírus Epstein-barr que foram submetidas ao Nested-PCR para identificação do genoma do vírus. O EBV foi identificado em 52(42%). Sendo detectado em 5/5 (100%) das leucoplasias pilosas orais, 10/52 papilomas,14/15(93%) carcinomas nasofaríngeos, 13/24 (54%) tonsilites e 10/29 (34%) linfomas. Na …

    brazil-uff Repository record

  2. Detection of the Oak Wilt Pathogen in Sapwood of Northern Oak Species Using PCR Technology

    Yang, Anna2015

    … involving the use of polymerase chain reaction (PCR) have the potential to offer faster and more reliable plant disease diagnostics. The purpose of this thesis was to 1) compare the use of a nested PCR and real-time PCR protocol to detect C. fagacearum in sapwood drill shavings using published …

    umn Repository record

  3. Real Time RT-PCR for Direct Detection of Viable Mycobacterium Avium Subspecies paratuberculosis in Chron's Disease Patients and Association of Map Infection with Downregulation in Interferon-Gamma Receptor (INFG1) Gene in Crohn's Disease Patients

    Chehtane, Mounir

    … in infected patients. The advent of real time PCR has proven to be significant in infectious disease diagnostics. In this study, real time reverse transcriptase PCR (RT-PCR) assay based on targeting mRNA of the IS900 gene unique to MAP has been developed. All variables included in RNA …

    ucf

  4. A Molecular Survey of Ehrlichia Chaffeensis and Ehrlichia Ewingii in Dogs and Ticks From Southwest Missouri

    Dixon, Matthew

    … as reservoirs for some species of Ehrlichia. Nested-PCR was used to survey for the presence of Ehrlichia chaffeensis and Ehrlichia ewingii in blood samples of 37 tick-infested dogs from southwest Missouri. Eleven percent of the asymptomatic dogs tested were positive for E. ewingii, which …

    mo-state Repository record

  5. The Prevalence of the Q-fever Agent Coxiella burnetii in Ticks Collected from an Animal Shelter in Southeast Georgia

    Smoyer, John H., III2014

    … to detect the Q-fever agent in these ticks, a nested PCR technique targeting the 16S rDNA of Coxiella burnetii was used. A collection of 450 ticks from the animal shelter were screened via nested PCR and 144 (32%) were positives. The positive PCR products were also confirmed by DNA sequencing. …

    gsu Repository record

  6. Screening for Pathogenic Escherichia Coli in the Chattahoochee River, Columbus, Georgia

    Staples, Micah A2017

    … above and below wastewater outputs. We used PCR to test for the individual subtypes and then used a nested PCR protocol for the human, bovine, and avian ETEC. Our results show that most isolated <em>E. coli</em> strains are not 0157:H7 or ETEC. However, nested PCR specific for human …

    columbus-state Repository record

  7. Avaliação de métodos baseados na reação em cadeia da polimerase (PCR), para o diagnóstico de pacientes em tratamento da malária

    de Albuquerque Montenegro, RosanaUniversidade Federal de Pernambuco2002

    … de detecção de DNA através das técnicas de PCR simples, hemi nested PCR (HNPCR) e hemi nested PCR em um único tubo (STHNPCR) em ensaios realizados com diluição seriada de sangue de pacientes infectados com Plasmodium falciparum. Comparamos a sensibilidade dos testes utilizando duas …

    brazil-ufpe Repository record

  8. Tahyna-Virus : Untersuchungen zum Vorkommen am Oberrhein und Sequenzvergleiche des M-Segments bei zehn Virusisolaten

    Schüßler, EvaUniversität Heidelberg

    … Das Virus wurde in den Stechmücken mit einer nested RT-PCR nachgewiesen. Die Stechmücken wurden homogenisiert und die RNA isoliert. Eine reverse Transkription folgte. Die cDNA wurde in einer nested PCR amplifiziert. Als Positiv-Kontrolle wurde Zellkultur-Überstand, der Tahyna-Viren enthielt, …

    heid-diss Repository record

  9. The Prevalence of Human Granulocytic Ehrlichiae in <em>Ixodes scapularis</em> Ticks in Southeastern Georgia

    Merten, Heather Anne2015

    … and mainland sites in southeastern Georgia using nested PCR techniques. Four locations were studied representing 2 barrier islands and 2 mainland sites. We hypothesized that areas that have low levels of human disturbance and are somewhat isolated will have a higher prevalence of infected ticks …

    gsu Repository record

  10. Incidencia y dispersión de virus transmitidos por pulgones en hortícolas de invierno y sus relaciones virus-vector

    Moreno Lozano, AránzazuAgronomos2005

    … También se puso a punto las técnicas de IC-RT-PCR e IC-RT-Nested-PCR, comparando su sensibilidad y especificidad. La técnica IC-RT-Nested-PCR se empleó para la detección del virus en pulgones, siendo posible la detección viral tanto en especies vectoras como no vectoras. En los estudios del …

    upm Repository record

  11. Detection of Giardia lamblia and Cryptosporidium parvum in the eastern oyster (Crassostrea virginica) harvested from the Great Bay Estuary

    Stowell, Kim Marie

    … kinetics. The development of a multiplex PCR assay achieved the specificity necessary to target the human pathogens and produce results more quickly then detection via immunofluorescence. A subsequent nested PCR assay prevented any potential false positive results.</p><p>To determine the …

    unh-thes Repository record

  12. Investigating the relationship between lung cancer and the infection with the parasite Toxoplasma gondii

    Tarabulsi, M2020

    … were investigated for T. gondii infection by nested PCR and immunohistochemistry (IHC), and it documented a striking 100% prevalence. We recruited ten lung samples via bronchoalveolar lavage from healthy individuals to be used as a control group for the lung cancer patients study. Samples were …

    salford Repository record

  13. HCV Resistenztestung

    Junge, JuliaUniversität Leipzig

    … der Resistenztestung wurde sich für die nested- bzw. seminested-PCR entschieden. Nach reverser Transkription der viralen RNA und anschließender Amplifikation der entstandenen cDNA wurde eine präparative Gelelektrophorese durchgeführt. Spezifische Banden wurden manuell extrahiert, …

    qucosa-diss

  14. T-RFLP analysis of bacterial 16S rRNA gene sequences isolated from river otter (Lontra canadensis) scat and parasite screening for the presence of Toxoplasma gondii

    Gustafson, Aubree Marie2009

    … both otters were amplified with a singe tube nested PCR primer set for the detection of the ITS 1 region of Toxoplasma gondii. Scat samples used in the T-RFLP analysis were also tested for the presence ofT. gondii using the same nested primer set. Neither the river otter tissue samples nor any …

    u-pacific Repository record

  15. Infection Rates of Amblyomma Americanum and Dermacentor Variablis by Francisella Tularensis and Borrelia Lonestari in Southwest Missouri

    Norton, Jacob Rhy

    … DNA was extracted from the ticks and used in nested PCR assays to identify F. tularensis using the fopA gene and B. lonestari using the flab gene. F. tularensis was present in 5.3% of adult A. americanum ticks (21/394) and 8.9% of adult D. variabilis ticks (17/192). A minimum infection rate of …

    mo-state Repository record

  16. Detection of non-native freshwater fishes using environmental DNA

    Davison, PhillipBournemouth University2023

    … still water body. A laboratory protocol based on nested PCR was developed, and determined in laboratory trials to be more sensitive than conventional or quantitative PCRs. This more sensitive technique was then applied to a repeat sampling at the fishing pond complex, confirming the previous …

    bournemouth Repository record

  17. Chondrogenic capacities of equine synovial progenitor populations

    Chen, Yu-WenUniversity of Illinois at Urbana-Champaign2012

    … phenotypes was assessed by quantitative RT-PCR of chondrocyte-specific genes, DMMB assays for deposition of sulphated glycosaminoglycans (sGAG), an ELISA assay for collagen type II (Coll II) deposition and the alkaline phosphatase (ALP) bio-assay. The results showed that equine synovial …

    uiuc Repository record

  18. Molekulinė naminių pelėdų (Strix aluco) hemosporidinių parazitų diagnostika /

    Žičkytė, Gabija,Institutional Repository of Vilnius University2024

    … Museum. DNA isolation with ammonium acetate and nested PCR revealed that 27 out of 76 individuals were infected with parasites. After sequencing, the parasites were identified using BLAST algorithms in the NCBI genebank and MalAvi database. The study showed that 35.53% of the individuals tested …

    vilnius Repository record

  19. Prevalenza delle infezioni da HPV in pazienti con infezioni delle ghiandole sessuali accessorie maschili MAGI

    CIANCIO, FABIO FRANCESCO MARIAUniversità degli studi di Catania2014

    … del DNA del HPV è stata fatta con HPV-HS Bio nested PCR per il rilevamento delle sequenze di DNA dell HPV contenenti L1 ORF. Nel gruppo A sono stati trovati 10 pazienti (20,8%) con infezioni da HPV Nel gruppo B sono stati trovati 15 pazienti con infezioni da HPV (28,9%). Complessivamente i …

    catania Repository record

  20. Novel Diagnostic approach for tuberculosis diagnosis

    Milovic, AnaDivision of Medical Microbiology2010

    … The GeneXpert system performs real time, nested PCR from sputum and provides a result within two hours of sampling. The result includes a semi-quantitative assessment of bacillary load in the sample and simultaneously detects rifampicin resistance. This study was part of a cross-sectional, …

    cape-town Repository record

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