Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"Neomycin resistance gene"”.
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Cationic liposome-mediated transfection of mammary epithelial cells with AAV-based plasmid DNA
… construct flanking a β-galactosidase reporter gene. LipofectAMINE was used to deliver this DNA to HC11 cells, however, the efficiency of transient transfection was only increased by a modest 1.4-fold. To evaluate the effect of AAV ITRs on plasmid DNA integration, a DNA construct containing a …
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The Association of the Human Cytomegalovirus Genome with the Cellular Nuclear Matrix
… regulatory region of the major immediate early gene (MIE) of the human cytomegalovirus (HCMV) has been analyzed for its capability of fulfilling criteria defining nuclear scaffold/matrix attachment regions (S/MARs). These criteria are: 1) binding the nuclear matrix in an in vitro nuclear matrix …
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Artificial Intron Technology to Generate Conditional Knock-Out Mice
<p>Genetic engineering has been re-shaped by the invention of new tools in modern biotechnology in a way that offers precision and efficiency in modifying the genome at a single nucleotide level and/or allowing precise control of gene expression. Such gene manipulation brings about significant …
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Generation of a CRISPR-Cas9 mediated knock-in reporter for the GRIA3 candidate gene for schizophrenia
… of Schizophrenia (SZ) and implicates genes in this pathway as potential candidates for the condition. A study on α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor genes found strong evidence of association between the Glutamate Ionotropic Receptor AMPA Type Subunit 3 …