Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Negative supercoiling"”.
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The effect of negative supercoiling on the formation and positioning of nucleosome cores in vitro
The effect of the negative supercoiling of DNA on the formation and positioning of nucleosome cores was investigated in a 1915bp plasmid (pHP2) containing a section of the early H1-H4 histone gene spacer of Psammechinus miliaris, previously shown to position the histone octamer (Retief et al., …
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Analysis of the structural changes caused by positive DNA supercoiling
… of supercoils in the DNA, with positive supercoiling (overwinding) generated ahead of the enzyme, and negative supercoiling (underwinding) in its wake. While the structural and physiological consequences of negative supercoiling have been well studied, technical challenges have prevented …
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A role for the Bacillus subtilis Structural Maintenance of Chromosomes (BsSMC) protein in chromosome organization and compaction
… the chromosome. Increasing the amount of supercoiling of DNA is a means to compact the chromosome. This thesis describes a role for BsSMC in supercoiling. I determined that BsSMC can alter the DNA topology of plasmids in vivo. There is also genetic evidence that BsSMC is involved in …
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DNA topology and Pol II CTD phosphorylation as stepwise regulators of chromatin architecture in differentiating human stem cells
… landscape for Pol II and its regulators, through supercoiling, non-B DNA formation, or altered loop anchors. The second is that Pol II activity is tuned by differentiation-specific phosphorylation of its C-terminal domain (CTD), which modulates interactions with co-transcriptional regulators and …
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The effects of nutrient limitation and growth rate on the regulation of the multi-drug pump genes acrAB in escherichia coli
… by cyclic AMP, and the possible role of negative supercoiling was discussed.
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EFFECT OF MISMATCHED BASE PAIRS ON DNA PLECTONEMES
… Magnetic-tweezers-based studies of DNA supercoiling, performed at high salt concentrations, have shown that in DNA harboring a single mismatch, the plectoneme will always localize at the mismatch. Theoretical studies have predicted that under physiological salt concentrations, plectoneme …
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Structure/function analyses indicate novel roles for mycobacterial DnaQ homologs in genome maintenance
… gyrases that are responsible for DNA negative supercoiling following replication. The phylogenetic analysis also revealed highly conserved nucleotide excision repair (NER) proteins among bacteria; however, some species like Actinobacteria, possess both a canonical UvrC along with a …
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The role of DNA supercoiling in the coordinated regulation of gene expression in Helicobacter pylori
… characterized yet. Changes in global or local supercoiling of chromosomal DNA are thought to play a role in global gene silencing and gene activation. In Helicobacter pylori, a bacterium with few dedicated transcriptional regulators, the structure of some promoters indicates a dependency on DNA …