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Showing 1 to 20 of 52 for “"Nef"”.
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Mechanisms of HIV-Nef Induced Endothelial Cell Stress: Implications of HIV-Nef Protein Persistence in Aviremic HIV Patients
… of HIV encoded pro-apoptotic protein, such as Nef in latently HIV-infected cells is a possible mechanism for vascular dysfunction underlying these diseases. HIVNef persists in two compartments in these patients: (i) extracellular vesicles (EV) of plasma and bronchoalveolar lavage (BAL) fluid …
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Functional Characterization of the HIV-1 NEF Acidic Cluster
… virus (SIV) encode an accessory protein Nef proposed to promote pathogenesis based on evidence from HIV-infected longterm nonprogressors and SIV studies in non-human primates. The pathogenic potential of Nef has been studied in relation to some of its in vitro effects including: (1) the …
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Etablierung eines infizierbaren Kleintiermodells zur Untersuchung von HIV-Nef
Das virale Nef-Protein ist eine wichtige Determinante für die Infektiosität und die Pathogenese des humanen und simianen Immundefizienzvirus. Die Bedeutung des Nef-Proteins wird durch Studien an nef-transgenen Mäusen, die eine AIDS-ähnliche Krankheit entwickeln, demonstriert. Um Funktionen von Nef …
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The mechanism of HIV-1 Nef-mediated downregulation of CD4
Nef, an accessory protein of HIV-1, is a critical determinant of viral pathogenicity. The pathogenic effects of Nef are in large part dependent on its ability to decrease the amount of CD4 on the surface of infected cells. Early studies suggested that Nef induces downregulation by linking the …
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Sam68, Stress Granules, and translational control of HIV-1 nef mRNA
… In this study, we focused on the viral factor Nef and the host factor Sam68. Nef is a major pathogenic viral determinant for HIV-1, and no therapeutics have been targeted to this factor. Sam68 is indispensible for HIV-1 propagation. We revealed that Sam68 variants were very potent in preventing …
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Structural and Functional Analysis of HIV-1 Nef Activation of PAK-2
Nef is an accessory protein encoded by HIV-1 that activates the host cellular p21 activated protein kinase 2 (PAK-2). Previous work has characterized the structural plasticity of Nef with regard to PAK-2 activation. Residues 89 and 191 were identified to be components of an effector domain required …
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The cellular and molecular basis of the Nef requirement for HIV-1 infectivity
Nef is an HIV -1 accessory protein with a fundamental role for virus replication in vivo and for the development of AIDS. Among its several activities, Nef is essential for full HIV-1 infectivity, a function highly prominent in lymphoid cells. So far, the mechanism by which Nef promotes HIV-1 …
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The biological effects of HIV-1 Nef on the development of B-cell Lymphoma
… to this study is the HIV-1 viral protein Nef (Negative Factor) which has been reported to have an oncogenic role in several cancer types including Kaposi's Sarcoma (KS) and Non-Small Cell Lung Cancer (NSCLC). However, the role of HIV-1 Nef in B-cell lymphoma, including BL, remains largely …
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Expression of HIV-1 subtype C nef in E. coli and Nicotiana benthamiana : development of plant-based vaccines for HIV
Expression of the nefgene from HIV-1 subtype C in Nicotiana benthamiana was carried out using a TMV -based vector with the aim of developing a plant-based candidate vaccine for HIV-1. The nef gene of the DU151 isolate of mV-l subtype C taken from a recently seroconverted individual was amplified by …
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Prokaryotic Production of Human Immunodeficiency VirusType 1 Subtype C Tat, Nef and Reverse Transcriptase andInvestigation of Antibody Responses to these proteins inHIV-1 Infected Individuals as well as Macaques Vaccinatedwith SAAVI DNA-C/C2 and SAAV
The purified Tat, Nef and RT recombinant proteins were used as antigens to investigate the prevalence of HIV-1 antibodies in sera of HIV-1 infected people. There was no reactivity in any of the 20 negative control sera in the Western blot assay. Analysis of 481 sera from HIV-1 infected individuals …
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Complement aberrations and autoantibodies to complement proteins in relation to disease mechanisms.
… alternative pathway, C3 nephritic factors (C3 NeF), cause partial C3 deficiency and are associated with increased susceptibility to bacterial infections. By analysis of samples from 20 patients with C3 NeF, it was confirmed that C3 NeF are of at least 2 types; one with fluid phase (C3 NeF type …
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Strukturelle Untersuchungen der Lck-Domänen unique und SH3 und ihre Wechselwirkungen mit zellulären und viralen Proteinen
… die Wechselwirkungen zwischen dem HIV-1 Protein Nef und dem HIV Hauptrezeptor CD4 gewonnen. Die unique- und die SH3-Domäne der Lck wurden in E. coli exprimiert und anschließend gereinigt. Die Untersuchung von LckU3 mittels NMR-Spektroskopie zeigte, dass die unique-Domäne in wässriger Lösung …
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FLUORESCENCE STUDIES OF EXCIPLEX MOLECULES IN THE VACUUM ULTRAVIOLET REGIME
… processes for both Ne$\sb 2$F* and its precursor NeF*, which emits at 108.5 nm, was performed. Several relevant rate coefficients were determined. It was concluded that this system is analogous to the Ar-Cl system, in that it predissociates due to excited halogen dimer formation. Therefore the …
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Protein/Peptide Recognition Modules in Cellular Signaling and HIV Pathogenesis
… As demonstrated by my biochemical study of HIV-1 Nef/SH3 domain interaction, regions of intact SH3 binding proteins outside the PxxP motif can enhance both affinity and specificity by targeting the highly divergent RT-loop of the SH3 domains. The crystal structure of Nef in complex with a …
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A Census of Human RNA-Binding Proteins and Characterization of the DEDDh RNA Exonuclease NEF-sp in the 3' End Maturation of 28S Ribosomal RNA
… highly tissue-specific RNA exonuclease NEF-sp and characterized its function in pre-28S ribosomal RNA processing. Ribosomal RNA biogenesis requires a series of endo- and exonucleolytic processing steps for the production of mature rRNAs. Although the mechanism of 28S 3' end rRNA …
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CALABI-YAU MANIFOLDS OF TYPE A AND THE CONE CONJECTURE FOR HYPERELLIPTIC VARIETIES
… in birational geometry, predicts that the nef and movable cones, of projective varieties Y with K_Y numerically trivial, admit a rational polyhedral structure under the action of automorphism and birational automorphism groups, respectively. The conjecture provides insights into both the …
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The risk appetite of development finance institutions (DFIs) and funding for start-ups in South Africa
… Partners (BP), the National Empowerment Fund (NEF) and the Small Enterprise Finance Agency (SEFA). This study analyses data between 2011 and 2015 to determine the DFI's risk appetite and to identify key determinants of risk appetite with regard to funding SMEs, specifically startups. The …
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Mechanisms of CD8+ T Cell Mediated Virus Inhibition in HIV-1 Virus Controllers
… cell responses with a prevalence of Gag p24 and Nef specificity among the virus controllers tested. Given this prevalence, we aimed to determine the gene expression profile of Gag p24-, Nef-, and unstimulated CD8+ T cells. RNA was isolated from CD8+ T-cells from two virus controllers with strong …
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