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Showing 1 to 11 of 11 for “"Nedd8"”.
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Regulation of NEDD8/Rub1 and its substrates
NEDD8 is a small ubiquitin like modifier (UBL) essential for life in almost all known eukaryotes. The primary objective of this work is to determine NEDD8 specific regulatory mechanisms. Like ubiquitin, NEDD8 is first matured and then conjugated via a tripartite conjugation cascade (E1, E2, E3). …
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Investigating the response of the NEDD8 ubiquitin-like molecule to diverse stress conditions
NEDD8 modification of proteins is extensively studied in the recent years, and the ubiquitin-like molecule has been shown to be involved in numerous signalling pathways. In addition to its well-established roles, we showed that NEDD8 responds to various stress conditions, such as inhibition of the …
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Structural Analysis of Cycle Inhibiting Factor from Pathogenic Escherichia Coli
… we identify the cell cycle regulatory protein Nedd8 as an interaction partner for Cif. Cif binds Nedd8 in vitro through both the catalytic domain and the N-terminal domain, the latter of which we demonstrate to be absolutely required for Cif activity in the host cell.</p>
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Novel Insights into Ubiquitin-like Protein E1-E2 Interactions
… proteins (UBLs) such as ubiquitin, Sumo and NEDD8 regulate a vast array of processes in the cell. Transfer of UBLs to their target generally occurs by a series of molecular handoffs down an E1‑E2‑E3 cascade. We are interested in understanding how E1‑E2 pairs interact and mediate UBL transfer. …
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COP9 signalosome subunit knockdown in K562 provides novel insight into the function and potential regulation of the CSN
… and removal of a ubiquitin-like protein called NEDD8 to and from the cullin subunit. Removal of NEDD8 (deneddylation) is mediated by the eight subunit COP9 signalosome (CSN; CSN1-8). Cullin deneddylation by the CSN has been demonstrated to prevent the autocatalytic degradation of the substrate …
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Phospho-regulation and metastatic potential of Murine Double Minute 2
… that can conjugate the ubiquitin-like molecule, nedd8, to p53. This modification results in inhibition of p53 activity, while maintaining p53 protein levels. While the signaling events that regulate Mdm2 E3 ubiquitin ligase activity have been extensively studied, what activates the neddylating …
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Characterising the mechanism of DCUN1D1 activity in prostate cancer and identifying DCUN1D1 inhibitors for prostate cancer treatment
… majority of the cullin family of proteins with NEDD8. This activity is known to enhance ubiquitination of the cullin RING E3 ligases, however, the extent of the impact of DCUN1D1's activity is underexplored. Studies performed previously in our lab demonstrated the role of DCUN1D1 in prostate …
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Characterising the mechanism of DCUN1D1 activity in prostate cancer and identifying DCUN1D1 inhibitors for prostate cancer treatment
… majority of the cullin family of proteins with NEDD8. This activity is known to enhance ubiquitination of the cullin RING E3 ligases, however, the extent of the impact of DCUN1D1's activity is underexplored. Studies performed previously in our lab demonstrated the role of DCUN1D1 in prostate …
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Defining the mechanism of arsenic-induced degradation of PML
… of the cullin RING ligase scaffold CUL3, and the NEDD8 E3 ligase DCUN1D1 resulted in striking accumulation of PML, suggesting PML may be a substrate of a CUL3 RING ligase complex. Further experiments using the inhibitor of neddylation, MLN4924 support this hypothesis.<br/>PML is expressed as a …
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Post Translational Regulation of AID Targeting to Both Strands of a Transcribed DNA Substrate
… work focuses on the role of the neddylation (Nedd8) of AID in recruitment to its target sequences. Neddylation, a 10kDa modifier, is a small ubiquitin like modifier which functions in a variety of cellular processes. We have used a combination of proteomics, computational approaches, and …
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Cell Fate Decisions in the Early Mammalian Embryo
… distributed proteins include Gps1 and Nedd8, depletion or overexpression of which in one blastomere of the 2-cell embryo impacts lineage segregation. Fascinatingly, halved mouse zygotes already display protein asymmetries, which resembles alpha and beta blastomeres, suggesting …