Global ETD Search
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Showing 1 to 7 of 7 for “"Ndc80 complex"”.
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Using biophysical analysis to study the regulation of the budding yeast kinetochore
… the kinetochore. The flexible outer kinetochore complex Ndc80 is essential to all of these functions. Previous studies have primarily focused on posttranslational modifications that affect regulation of the complex. I sought to determine how additional kinetochore components might regulate the …
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Dissecting the Spatiotemporal Regulation of Yeast Kinetochore Interactions
Kinetochores are conserved protein complexes that bind the centromeres in replicated chromosomes to the mitotic spindle and then direct their segregation. To better comprehend Saccharomyces cerevisiae kinetochore function, we investigated the phospho-regulated dynamic interaction between the …
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Organization and regulation of outer kinetochore assembly
… directs chromosome segregation. The KNL1/Mis12 complex/Ndc80 complex (KMN) network acts as the primary microtubule-binding interface at kinetochores, and provides a platform for recruitment of regulatory proteins. The results in this thesis demonstrate that the CENP-C and CENP-T inner …
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Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… an E3 ubiquitin ligase called anaphase-promoting complex. The roles of two kinases- BubR1 and Mps1, implicated in spindle assembly checkpoint were investigated. A checkpoint complex containing BubR1 and Bub3 has been purified from mitotic human cells. BubR1 directly interacts with Cdc20 and …
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Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… an E3 ubiquitin ligase called anaphase-promoting complex. The roles of two kinases- BubR1 and Mps1, implicated in spindle assembly checkpoint were investigated. A checkpoint complex containing BubR1 and Bub3 has been purified from mitotic human cells. BubR1 directly interacts with Cdc20 and …
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Mechanistic Insights into the Microtubule-Mediated Differential Modulations of Aurora B Substrate Structures for Accurate Chromosome Segregation
… the kinase subunit of the Chromosomal Passenger Complex (CPC). How Aurora B can distinguish between erroneous and correct attachments and selectively phosphorylate its key substrates in the right contexts remains poorly understood. The Ndc80 complex (Ndc80C) is a key substrate of Aurora B for …
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Kinetochore-Microtubule interface: analyses of Ndc80-Dam1 Interactions
… (crucial step for bi-orientation) are the Ndc80 and Dam1 complexes. The Ndc80 complex consists of four subunits: Ndc80, Nuf2, Spc24 and Spc25 and is rod- shaped. The Dam1/DASH complex is composed of ten proteins, including Dam1, and forms a ring structure around microtubules. The Ndc80 and …