Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 14 of 14 for “"Ndc80"”.
-
Kinetochore-Microtubule interface: analyses of Ndc80-Dam1 Interactions
… (crucial step for bi-orientation) are the Ndc80 and Dam1 complexes. The Ndc80 complex consists of four subunits: Ndc80, Nuf2, Spc24 and Spc25 and is rod- shaped. The Dam1/DASH complex is composed of ten proteins, including Dam1, and forms a ring structure around microtubules. The Ndc80 and …
-
Using biophysical analysis to study the regulation of the budding yeast kinetochore
… The flexible outer kinetochore complex Ndc80 is essential to all of these functions. Previous studies have primarily focused on posttranslational modifications that affect regulation of the complex. I sought to determine how additional kinetochore components might regulate the …
-
Dissecting the Spatiotemporal Regulation of Yeast Kinetochore Interactions
… Cnn1CENP-T, the centromere region and the Ndc80 complex through the cell cycle. Cnn1 localizes to kinetochores at basal levels from G1 through metaphase but accumulates abruptly at anaphase onset. How Cnn1 is recruited and which activities regulate its dynamic localization is unclear. We …
-
Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… congression). Components of the conserved Ndc80 complex have been implicated in both these function. To better understand the function of the Ndc80 complex, we have identified two novel subunits of the human Ndc80 complex, termed human Spc25 (hSpc25) and human Spc24 (hSpc24), using an …
-
Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… congression). Components of the conserved Ndc80 complex have been implicated in both these function. To better understand the function of the Ndc80 complex, we have identified two novel subunits of the human Ndc80 complex, termed human Spc25 (hSpc25) and human Spc24 (hSpc24), using an …
-
Funktionelle Charakterisierung und Positionierung von Drosophila Cenp-C im Zentromer/Kinetochor-Komplex
… Zu den transienten CKC-Komponenten gehören der Ndc80- und der MIND-Komplex. Die Identifizierung der Drosophila Ndc80- und MIND-Komplex-Untereinheiten (Ndc80, Nuf2, Spc25, Mis12, Nsl1, Nnf1a, Nnf1b) in dieser Arbeit bekräftigt die Existenz einer konservierten CKC-Struktur. Überdies wurden …
-
Organization and regulation of outer kinetochore assembly
… chromosome segregation. The KNL1/Mis12 complex/Ndc80 complex (KMN) network acts as the primary microtubule-binding interface at kinetochores, and provides a platform for recruitment of regulatory proteins. The results in this thesis demonstrate that the CENP-C and CENP-T inner kinetochore …
-
Role of phosphatases in the end-on conversion process
… does not. I also investigated whether the HEC1/Ndc80 N-tail is a critical substrate of Aurora B phosphorylation for the stabilisation of the end-on attachments. Using a phospho-dead mutant of the HEC1/Ndc80 N-tail, I discovered that cells are still susceptible to Aurora B activity, indicating …
-
Mechanistic Insights into the Microtubule-Mediated Differential Modulations of Aurora B Substrate Structures for Accurate Chromosome Segregation
… right contexts remains poorly understood. The Ndc80 complex (Ndc80C) is a key substrate of Aurora B for regulating kinetochore-microtubule attachments. Ndc80C with Hypo-phosphorylated Ndc80 subunit (Hec1 in humans) binds microtubules with high affinity, promoting stable kinetochore-microtubule …
-
Impacts of sedimentation on early-life phenotypes and gene expression in coho salmon (Oncorhynchus kisutch)
… and four hypoxia-specific (Anillin, Ncapd3, Ndc80, Kif4) biomarkers revealed significant upregulation of ALD_1 expression in hypoxia-reared fish, while fewer significant differences were detected in the remaining biomarkers. Overall, more research is necessary to elucidate the genomic …
-
Regulation of microtubule attachment at the kinetochore and cell cortex
… KNL-1, a component of the KMN (KNL-1, Mis12, Ndc80) network, and the kinetochore and microtubule-associated Astrin/SKAP complex. KNL-1 is a kinetochore specific scaffolding protein that I show to have the ability to form large oligomers in nematodes. The Astrin/SKAP complex has the unique …
-
Molecular Mechanisms of Mitotic Spindle Assembly and Accurate Chromosome Segregation
… a decreased level of Aurora B-mediated Ndc80 phosphorylation at the kinetochore of cells expressing the non-phosphorylatable BubR1 mutant, which might contribute to the alignment defect. Moreover, expressing a phosphomimetic BubR1 mutant substantially reduces the incidence of polar …
-
Role of Ame1, a central component of the Saccharomyces cerevisiae kinetochore, in kinetochore function and structure
… network between the Okp1 / Mtw1 / Spc105 / Ndc80 kinetochore complexes could be established by in vitro binding assays performed with isolated protein complexes. • These data together with those derived from ChIP analyses of the ame1-2 mutant show a clear dependency of the centromeric …