Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"Natural substrate"”.
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Enzyme Kinetics of Recombinant Human Arylsulfatase B (rhASB)
… using an in vitro bioassay and direct hydrolysis substrate assay to approach the problem of substrate limitation in establishing a representative kcat for rhASB. The project also explores the makeup of a possible ASB complex within the cell. Previous published studies have sought to establish a …
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Local effects of culverts on habitat features and fish assemblages in Blue Ridge streams
… sites contained culverts with artificial bottom substrates and five sites contained culverts with natural bottom substrates. On each stream, paired sampling reaches (reach length ~35 times mean reach width, drainage area <11 km 2) were established 50 m upstream and 50 m downstream of the culvert. …
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Structural and Dynamic Aspects of the Cytochrome P450(cam) Protein Conformation
… high-spin (S = 5/2) form upon binding the natural substrate camphor. Cytochrome P-450$\sb{\rm cam}$ exhibits a distribution of spin states when various camphor analogs are bound to the cytochrome. These camphor analogs provide a means of independently varying the ferric spin equilibrium of …
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Response of indigenous heterotrophic groundwater bacteria to low organic substrate availability
… rely on dissolved low molecular weight organic substrates in dilute solution for their nutrition. Periods of metabolic dormancy may occur when natural substrate concentrations drop below the level required to sustain vegetative cell function. In these studies total cells present were determined …
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In silico investigation of the mechanism of ricin-catalysed depurination reaction and design of novel ricin inhibitors
… reaction, a reasonable model of the target natural substrate is required.
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The synthesis and in vitro evaluation of short interfering RNAs that contain internal modified alkyl spacers
… backbone or due to the fact that RNA is a natural substrate for nucleases. In an attempt to mitigate these problems there has been considerable focus on chemical modification of siRNAs such as alteration of the ribose ring or backbone. Backbone alterations such as abasic alkyl linkers have …
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Electrochemistry of soluble methane monooxygenase on a modified gold electrode : implications for chemical sensing in natural waters
… catalyzes the oxygenation of methane and other substrates, to design a methane sensor for use in natural waters. Such a sensor would be useful for monitoring lake biogeochemistry, including the potential for lakes to be a source of methane into the atmosphere. An op amp-based potentiostat was …
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Mechanism of Substrate Specificity and Catalysis in Retaining β-Glucosidases From Maize and Sorghum
… Glu1and Glu2 hydrolyze a broad spectrum of substrates in addition to its natural substrate DIMBOAGlc, while the sorghum β-glucosidase Dhr1 (dhurrinase-1) hydrolyzes exclusively its natural substrate dhurrin. For the expression of mature β-glucosidase isozymes Glu1 and Glu2 of maize and Dhr1 …
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Synthesis of inhibitors of polysialyltransferases PST and STX. Development of routes to synthesis, preparation and purification of carbohydrate and carbacycle-based potential inhibitors of the polysialyltransferase enzymes PST and STX
… that are structural analogues of CMP-Neu5Ac, the natural substrate. Specifically, development of methodology to synthesise building blocks suitable for conjugation as inhibitors is described. Quinic acid-based substrate analogues were explored, with a focus on development of chemistry to achieve …
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Mechanistic investigation of polyhydroxybutyrate (PHB) synthases and elucidation of PHB biosynthesis and degradation process in Wautersia eutropha H16
… involved in polymerizations in which a soluble substrate is transformed into an insoluble inclusion during the polymerization process. The initiation, elongation, and termination phases of this non-template driven polymerization process are not well understood. This thesis is focused on the …
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Studies within Fragment-Based Drug Discovery: Library Synthesis and Hit-to-Lead Optimisation
… and occupying it with a fragment mimicking its natural substrate. This approach yielded a compound with an in vitro half maximal inhibitory concentration of 130 µM against the enzymatic activity of PrpC.
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Enzymatic Electrode Design: A Systematic Approach to Enhanced Bioelectrocatalysis
… the interaction between an enzyme and its natural substrate and the other involving the interaction of an electric field with the enzyme dipole moment. The internal electron transport chain of a specific group of redox enzymes is explored and then mimicked on the surface of the electrode to …
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The Development and Assessment of Computational Approaches to the Thermodynamics and Kinetics of Binding
… and proteins, proteins and proteins, enzymes and substrates, and nucleic acids with each other. Many cellular processes are governed by a group of proteins acting in a coordinated manner; such complicated mechanisms are closely regulated: changes in the populations of particular complexes or …
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Development of a Cellular Model for Morquio A Syndrome
… of a functional bioassay for rhGALNS using the natural substrate, lumican, of the enzyme.</p>
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Design and Synthesis of Inhibitors of Mycobacterium tuberculosis Menaquinone D (Mtb MenD) as Potential Therapeutics for Tuberculosis (Tb)
… series of inhibitor candidates derived from the natural substrate isochorismate were synthesized. Unexpectedly, these were shown by X-ray crystallography to bind to a previously unreported allosteric regulation site on MenD, which was identified by our collaborators during the period of this …
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Current understanding on cytochrome bd quinol oxidase of Escherichia coli a mutagenesis, kinetics and spectroscopic study
… was carried out by stopped-flow techniques. The natural substrate, ubiquinol, was used to turnover the enzyme in the fast catalysis successfully for the first time. The results excluded the fully oxidized form of the enzyme from the rapid catalytic cycle of cytochrome bd oxidase. A …
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Rational design and directed evolution of probe ligases for site-specific protein labeling and live-cell imaging
… had severely decreased activity for LplA's natural substrate, lipoic acid. We created a 107 library of LplA mutants and, after four rounds of selection, produced a truncated LplA mutant with lipoylation activity equivalent to full-length LplA. We next sought to evolve activity for an …
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Wild Tigers in Captivity: A Study of the Effects of the Captive Environment on Tiger Behavior
… do not allow them to carry out their natural behaviors (such as swimming, climbing, stalking, and predation). Captive environments in zoological parks often do not provide for natural behaviors due to spatial constraints and negative public reaction. Tigers (Panthera tigris) present a …
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Rekombinante Biosynthese amino-substituierter Phenylpropanoide in E. coli
… groups and it has significant similarity to the naturally occurring monolignol p-coumaryl alcohol. Unlike this substance there is no existing pathway known for the biosynthesis of the derivate in natura. The approach to design an artificial pathway for p-aminocinnamyl alcohol is based on the …
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A study of the mechanisms of antagonism by the biocontrol fungi <i>Trichoderma </i>against wood decay basidiomycetes
… with wood decay basidiomycetes in the natural substrate, i.e., wood. Statistical comparisons were carried out between the % weight loss of wood blocks after exposure to selected basidiomycetes, with individual antagonistic responses observed against these same wood decay fungi using …
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