Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Native mass spectrometry."”.
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Studies of hyperphosphorylated tau aggregation and cytotoxicity
… aggregates in vitro. We employed tandem mass spectrometry to determine the phosphorylation sites, highresolution native mass spectrometry to measure the degree of phosphorylation, and superresolution microscopy and electron microscopy to characterize the morphology of aggregates formed. …
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Analysis of membrane systems using lipoprotein nanodiscs
… lipoprotein Nanodiscs provide a well-defined and native-like lipid bilayer to solubilize membrane proteins and lipids. This dissertation details the analysis of Nanodiscs containing various membrane proteins and lipids with bioanalytical methods, including mass spectrometry and photonic …
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Characterizing Conformational Dynamics and Catalytic Activity of Enzymes by Hydrogen-Deuterium Exchange Mass Spectrometry
… activity on the millisecond to second timescale. Native mass spectrometry enabled studies into binding affinity and monitoring of substrate inactivation on a measured time course. Ion mobility mass spectrometry (IM-MS) was used to provide definitive MS/MS results for protein coverage and provide …
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Expression, Purification and Molecular Characterisation of Sideroflexin Proteins
… zinc ions, which was confirmed subsequently by native mass spectrometry and elemental mass spectrometry. Following this result, the groundwork was laid to find the zinc binding site by combining scanning alanine mutagenesis with thermostability assays. Furthermore, all five human paralogues and …
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Getting Insight into the Molecular Determinants of Foldopathies
… in the presence of Copper(II) and Acrolein using mass spectrometry for the determination of the interaction site which was Histidine 50. The site was further confirmed by additional studies with circular dichroism, dynamic light scattering and synthesized short peptide containing Histidine 50. …
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Investigation of mass spectrometry approaches for aiding fragment-based drug discovery
The use of mass spectrometry (MS)-based techniques to study protein structure dynamics is a relatively new field, with seminal developments such as nanoelectrospray ionization (nano-ESI) and traveling-wave ion mobility-mass spectrometry (TWIM-MS) MS being introduced only around twenty-five and …
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Investigating structural and stability characteristics of native-like proteins using ion mobility-mass spectrometry (IM-MS) in positive- and negative-ion mode.
Native ion mobility-mass spectrometry (IMS-MS) is widely used to investigate the structure and stability of biologically relevant proteins and protein complexes. The key goal, and arguably biggest attraction, of native IMS-MS is maintaining the noncovalent interactions responsible for stabilizing …