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Showing 1 to 12 of 12 for “"Native Chemical Ligation"”.
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Synthesis of Epidermal Growth Factor by Native Chemical Ligation and Incorporation of Non-Natural Amino Acids
… complete dodecapeptide synthesis. Chemoselective ligation techniques allow the use of unprotected peptide fragments which can eliminate solubility problems associated with fragment condensation. Native chemical ligation results in a peptide bond thus, joining the fragments. Therefore, it was …
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Synthesis of Selenium-Alkylated Selenocysteine Derivatives and Their Use in Native Chemical Ligation, Expressed Protein Ligation and the Synthesis of Dehydropeptides
Finally, an improved, scalable synthesis of Fmoc-phenylselenocysteine is reported. The amino acid was coupled to a model peptide on the solid phase. It was demonstrated that the selenide could be oxidatively eliminated to give a dehydropeptide while still bound to the solid resin. In addition, the …
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Induktion von Sekundärstrukturen durch den Einbau von Alanyl-PNA in Peptide und Proteine
… der Einbau von Alanyl-PNA durch die Technik der native chemical ligation in größere Peptide möglich ist. Hierfür wurde der N-terminale Thioester 31 des humanen Interleukins hIL8(1-55) durch Expression des Fusionsproteines in E.coli und Expressed Protein Ligation dargestellt. Nach Umsetzung des …
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Caged phosphopeptides and phosphoproteins : probes to dissect the role of phosphorylation in complex signaling pathways
… at residue 31 of the 557-residue protein using native chemical ligation.
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Convergent Site-Selective Carbohydrate-Peptide Ligations With Dehydroalanine and Aziridine -2 -Carboxylic Acid-Containing Peptides
… reactions. The efficiency of the dehydroalanine ligation approach was demonstrated by the preparation of thio-isosteres of the four tumor-associated carbohydrate antigens, T N, T, STN, and 2,6-ST, as a pair of diastereoisomers at the newly formed cysteine stereocenter. This 1,4-conjugate addition …
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Chemical Modification Methods for Protein Misfolding Studies
… proteins for misfolding studies. We adopted the native chemical ligation (NCL) method between a C-terminal thioester fragment and an N-terminal Cys fragment. We first devised strategies for the synthesis of thioamide-containing peptide thioesters as NCL substrates, and demonstrated their …
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Synthesis and characterisation of peptide-based probes for quantitative multicolour STORM imaging
… anti-GFP and ant-mCherry nanobodies via native chemical ligation. The labelled anti-GFP nanobody was to demonstrate ensemble and single molecule imaging in S. pombe, as well as characterisation on single molecule surfaces in comparison to a conventional randomly labelled antibody. The …
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Examining Post-Translational Modifications In Alpha-Synuclein Through Semi-Synthesis And Single Molecule Spectroscopy
… acids, and semi-synthetic strategies involving native chemical ligation (NCL) are optimized for the incorporation of challenging or novel modifications such as phosphorylation, arginylation, and acetylation. Where applicable, we evaluate trade-offs between the synthetic ease of using a mimic and …
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Investigation and application of heterochiral proteins enabled by flow-based peptide synthesis
… Solid-phase peptide synthesis (SPPS) and native chemical ligation enable the total chemical synthesis of proteins. Using these techniques, it is possible to design and study polypeptides foreign to Nature. Herein, I describe the investigation and application of proteins simultaneously …
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Adiciones 1,4-conjugadas a deshidroalaninas quirales para la síntesis de aminoácidos no naturales
… derivative of selenohomocysteine to be used in native chemical ligation is described. In addition, an amino acid bearing an alkyne group susceptible to click reactions with azides is also obtained, which can be used in bioconjugation chemistry. In Chapter 5, the synthesis of different compounds …
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A study on the interactions of synthetic IGF-II analogues with the type 1 IGF and insulin receptors.
… IGF-II protein. This was followed by an improved chemical synthesis of the Fl9Cou IGF-II protein using a linear solid phase peptide synthesis (SPPS) approach and is detailed in Chapter 4. A robust native chemical ligation approach was developed in Chapter 5, which allowed for the facile …
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Metalloprotein engineering with unnatural amino acids: application in functional heme-copper oxidase and azurin
… cavity complementation, total synthesis, native chemical ligation, expressed protein ligation and amber suppression. They all have their own advantages and disadvantages. Heme copper oxidases (HCOs) catalyzes reduction of O2 to H2O and harvest energy at the end of respiration chain. The …