Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 7 of 7 for “"Nanoscopy"”.
-
Probing surface protein patterning in biological systems using fluorescence nanoscopy
… microscopy. Recently developed fluorescence nanoscopy techniques such as stochastic optical reconstruction microscopy (STORM) effectively break the diffraction limit (~250 nm) to enable imaging with an order of magnitude smaller than the spatial resolution. In this work, we applied …
-
Structural characterization of primary cilia using accelerated piezoelectrically driven STED nanoscopy
… well as hiPSCs, we developed a superresolution nanoscopy system using the stimulated emission depletion (STED) technique with novel accelerated piezoelectric control (apSTED). This improved STED system achieved a reduction in photobleaching rates from ~80% to ~10% while maintaining …
-
Nanolithography and nanoscopy methods for the study of biological samples in confined spaces
Alzheimer’s, Parkinson’s and Huntington’s disease all belong to the group of amyloid pathologies also called protein misfolding diseases. Since the first discovery of amyloid fibrils of the aggregated protein tau in inclusion of Alzheimer brains samples, research has focussed on how amyloids form …
-
Super-Resolution Fluorescence Microscopy using Transparent Waveguide Chips
Super-resolution fluorescence microscopy (optical nanoscopy) allows us to surpass the optical diffraction limit and observe biological phenomena at the nanoscale using visible light. Different modalities come with their own trade-offs, such as imaging speed vs. achieved resolution or live cell …
-
A general method for three color STED microscopy with one depletion laser : application to primary neuronal culture
Recent advances in the field of optical nanoscopy have equipped molecular neuroscientists with the means to investigate sub-synaptic phenomena with unprecedented spatial resolution. Indeed, early studies employing these methodologies have revealed an extraordinary level of nanoscale spatiotemporal …
-
Adaptive optics stimulated emission depletion microscope for thick sample imaging
… diffraction barrier and opening the new era of nanoscopy. Among the fluorescent super-resolution techniques, Stimulated Emission Depletion (STED) microscopy has been particularly interesting, as it is a purely optical technique which does not require post image processing. STED microscopy has …
-
Development of Active Terahertz Modulators for use with Quantum Cascade Lasers and Near-Field Imaging of their Individual Resonators
This thesis describes the development of active terahertz modulators based on arrays of coupled resonators loaded with monolayer graphene. The structures affect the amplitude, phase, and polarisation of the transmitted and reflected radiation. These devices were simulated using finite element …