Global ETD Search
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Showing 1 to 20 of 74 for “"Nanog"”.
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Cryo-electron microscopy studies of pluripotency factor Nanog
… a group of transcription factors which includes Nanog, Sox2 and Oct4. Nanog is a homeodomain protein which, when over-expressed, can drive cytokine-independent self-renewal of undifferentiated ES cells. Dimerisation of Nanog through the tryptophan repeat (WR) region, a low complexity domain …
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Instructing naïve pluripotency ; JAK/STAT3 and Nanog in reprogramming
… The pluripotency associated transcription factor Nanog has been subject to a lot of controversy. Initially thought to form an essential trinity with Oct4 and Sox2 to maintain embryonic stem cell (ES) self-renewal, it was shown that actually ES cells can maintain their pluripotency in the absence …
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ROLE OF A SPECIFIC NANOG SUPER-ENHANCER ERNA IN EPIGENETIC REMODELING AND ENHANCER-PROMOTER INTERACTION
… transcribed from the region 5kb upper the TSS of Nanog gene in mESCs has been investigated. In particular, a specific eRNA transcript, here defined as antisense eRNA, resulted actively involved in the maintenance of Nanog gene in an active epigenetic state. After the antisense eRNA silencing, …
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Hyperosmotic Stress Enzyme Signaling Modulates Oct4, Nanog, And Rex1 Expression And Induces Prioritized Differentiation Of Murine Embryonic Stem Cells
… STRESS ENZYME SIGNALING MODULATES OCT4, NANOG,</p> <p>AND REX1 EXPRESSION AND INDUCES PRIORITIZED DIFFERENTIATION OF</p> <p>MURINE EMBRYONIC STEM CELLS</p> <p>by</p> <p>JILL SLATER</p> <p>MAY 2013</p> <p>Advisor: Daniel Rappolee, Ph.D.</p> <p>Major: Physiology</p> <p>Degree: Doctor of …
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Roles of Nanog, a transcription factor for self-renewal of embryonic stem cells, in prostate tumor initiation and chemoresistance
… initiation by cancer stem cells is unknown. Nanog, along with Oct3/4 and Sox-2, constitute the core transcriptional circuitry for the maintenance of stemness in embryonic stem cells. Herein we report that Nanog expression was detected at mRNA and protein levels in prostate cancer cells. The …
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Expresión de SOX2 y Nanog en lesiones orales potencialmente cancerizables y en carcinoma de células escamosas de cavidad oral. Significado clínico y pronóstico
… de progresión a carcinoma de estas lesiones. NANOG y SXO2 son reguladores clave de la pluripotencialidad y autorrenovación de las células troncales (stem) en tejidos embrionarios adultos
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NANOGP1 as a Model to Study the Consequences of Gene Duplications on Human Pluripotency and Development
… To address this knowledge gap, I investigated NANOGP1, which is a duplicated pseudogene of a key pluripotency factor called NANOG. NANOGP1 was chosen as a model for studying gene duplication in human pluripotency for several reasons. Firstly, NANOGP1 is an evolutionarily conserved duplicate in …
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Single-cell analyses of cellular reprogramming and embryonic stem cells
… cells without Oct4, Klf4, Sox2, c-Myc, and Nanog. Embryonic stem cells (ESCs) are the gold standard comparison for iPSCs. Our investigation of ESCs must continue in parallel to that of iPSCs since we cannot truly understand iPSCs if we do not understand the molecular mechanisms that regulate …
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Understanding Nanog'S Role In Cancer Biology
<p><strong>Understanding Nanog’s Role in Cancer Biology</strong></p> <p><strong></strong>Mark Daniel Badeaux</p> <p>Supervisory Professor Dean Tang, PhD</p> <p>The cancer stem cell model holds that tumor heterogeneity and population-level immortality are driven by a subset of cells within the …
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Molecular control of embryonic stem cell identity
… key pluripotency transcription factors, Oct4 and Nanog. The data from this study have also been compared and integrated with Oct4 and Nanog DNA binding data obtained in a different study using the ChIP-PET technology. In order to gain further insight into the mechanisms by which the transcription …
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Germ cell nuclear factor is not required for the down-regulation of pluripotency markers in fetal ovarian germ cells
… expression of the pluripotency markers Oct4 and Nanog longer than any other cells in the body. While somatic cells repress these markers during gastrulation, female germ cells continue to express them until around the time of meiotic initiation. It is not yet clear why pluripotency markers are …
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Maintaining Proper Levels of DNA Methylation Marks Through the TET Family is Critical for Normal Embryo Development in Pigs
… resulted in an increased expression of NANOG and ESRRB genes in blastocysts, although the DNA methylation levels of NANOG promoter were not changed. Interestingly, transcript abundance of TET3 was increased in blastocysts carrying inactivated TET1, which might have had an effect on the …
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Role of cancer stem cells in breast and prostate cancer
… with the aim of identifying CSC-like cells via a nanog-reporter cell line and enable the subsequent targeting of these cells with CSC-targeting agents. SUM159 and MCF7 cell lines cultured in 3D cell culture significantly enriched for the CD44+/CD24- breast cancer stem cell phenotype when compared …
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Regulation and patterning of cell differentiation and pluripotency
… cultured in a microfluidic 3. Patterning of the Nanog gene expression in embryonic stem cells, using a microfluidic device, to establish differentiation - pluripotency boundaries that mimic the developmental processes in vivo. In the first project, we developed a novel strategy for controlled …
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In Vitro Models of Cellular Dedifferentiation for Regenerative Medicine
… for pluripotency marker (Oct4, Sox2, and Nanog) expression, PCR for upregulation of endogenous pluripotency factors (Oct4, Sox2, cMyc, Klf4, and Nanog), and the ability to form embryoid bodies that expressed markers of all three germ layers. Additionally, we were able to create secondary …
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Cellular decision making: from phage lambda to stem cells
… regulated, Oct4 and Sox2, while the third, Nanog, exhibits large variability among the population. Additionally, the level of Nanog has been correlated with the stability of pluripotency. The reasons for the variability in Nanog level are not known, but stochastic gene expression has been …
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Δnp63 Regulates A Complex Network of Target Genes In Limb and Epidermal Development
… pluripotency regulators, Oct4, Sox2 and Nanog directly through the regulation of DGCR8. I found that DGCR8 restoration resulted in repression of Oct4, Sox2 and Nanog in ΔNp63-/- epidermal cells and rescue differentiation defects. Loss of ΔNp63 resulted in pluripotency that caused defect …
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Supplementing Bovine Embryo Culture Media to Improve the Production and Quality of In Vitro Produced Bovine Embryos
… IL6 was largely comprised of hypoblast (GATA6+:NANOG-) cells, and most IL6-responsive cells in day 9 blastocysts were hypoblast cells (as measured by STAT3 activation). However, some epiblast (NANOG+) cells were also IL6-responsive, and IL6 appeared to initially slow epiblast differentiation. …
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Mapping the core regulatory circuitry of embryonic stem cells
… the key pluripotency regulators, Oct4, Sox2 and Nanog. We then determined the genes targeted by the Polycomb Repressive Complex in ES cells. These datasets allowed us to define the core transcriptional regulatory circuitry for these cells and demonstrated that pluripotency is mediated through the …
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