Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 40 for “"Nanodiscs"”.
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Analysis of membrane systems using lipoprotein nanodiscs
… challenging. Engineered nanoscale lipoprotein Nanodiscs provide a well-defined and native-like lipid bilayer to solubilize membrane proteins and lipids. This dissertation details the analysis of Nanodiscs containing various membrane proteins and lipids with bioanalytical methods, including mass …
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Incorporation and analysis of bilayer constituents in nanodiscs
Nanodiscs, engineered lipoparticles composed of a lipid bilayer and two surrounding amphipathic proteins, have been utilized to house and solubilize integral membrane proteins and other molecules with large hydrophobic groups which limit their aqueous solubility. In the experiments described …
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Antiferromagnetic nanodiscs with perpendicular magnetic anisotropy for biological applications
… of particularly high yields of well-defined nanodiscs with robust magnetic properties. Abstract The magnetic behaviour of the PM SAF particles, and the thin films they are created from, is analysed with particular focus on the characteristics displayed in a fluidic environment. This leads to …
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Methodologies for the analysis of membrane systems using lipid nanodiscs
The student, Ivan Lenov, accepted the attached license on 2016-05-24 at 11:01.
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Protein -Lipid Interactions in Soluble Nanoscale Lipid Bilayers
"Finally, a method to rapidly pattern and assay Nanodiscs using microfluidics was developed. The technique uses microfluidic channels to pattern rows of Nanodiscs on a glass slide, and then deliver multiple analytes in perpendicular rows to create a ""matrix"" assay. This successfully characterized …
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Multiplex analysis of protein-lipid and protein-membrane protein interactions utilizing silicon photonic microring resonators
… blood coagulation cascade. To accomplish this, nanodiscs were integrated with silicon photonic microring resonators. Nanodiscs are easy to assemble, nanometer scale lipid bilayer discs which offer a high degree of control over lipid and membrane composition. Each nanodisc is held together by two …
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Multidimensional Solid-State NMR Studies of Membrane Proteins
… In order to evaluate the potential of utilizing Nanodiscs for solid-state NMR study of membrane proteins, we first prepared samples of empty Nanodisc with the membrane scaffold protein isotopically labeled. We demonstrated that precipitated Nanodisc samples show narrow inhomogeneous linewidths, …
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Dynamics of protein-membrane interactions in drug metabolism and cancer
… interactions properly. Phospholipid Nanodiscs provide an excellent solution to this problem, and they allow one to control protein and lipid stoichiometry with precision. In this dissertation, I will apply Nanodisc technology to explore physiologically relevant interactions between …
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Understanding Ultrafast Energy Transfer across the Photosynthetic Membrane of Purple Bacteria with Near-Native Systems
… are made possible by the use of model membrane nanodiscs, through which I reconstruct the antenna network piece-by-piece to resolve key energy transfer steps in a controlled, near-native environment. Overall, the results show that the energy transfer dynamics within the purple bacterial antenna …
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The membrane lipid composition regulates talin mediated integrin activation
… with bilayers of controlled composition using Nanodiscs technology. Results show that recruitment of talin head domain (THD) to the membrane surface is governed by electrostatics in the absence of other adapter proteins. In addition, distance measurements reveal that anionic lipids stimulate a …
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Polyprenyl-dependent glycan assembly pathways in microbial pathogens
… by employing a model membrane system, termed Nanodiscs, which comprises a discoidal phospholipid bilayer encircled by a scaffold protein. In this thesis, the glycosyltransferases responsible for the first two committed membrane steps in the Campylobacter jejuni N-linked protein glycosylation …
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Investigating the C-terminal region of RalA: regulation through membrane dynamics and protein-protein interactions
… This project uses solution NMR spectroscopy and nanodiscs as membrane mimetic tools to investigate the interaction of RalA with biological membranes at the molecular level. Clear differences were found in how RalA interacts with the membrane mimic based on the nucleotide status of the protein and …
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Structure-function studies of respiratory complex I from Paracoccus denitrificans using membrane mimetics
… competence of the nanodisc-bound enzyme. Nanodiscs thus provide a new tool to alleviate the inherent instability of the bacterial enzyme and enable the preparation of high-quality cryo-EM samples for structural investigations. Next, the first high-resolution structure of isolated P. …
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Reconstitution and Mechanistic Studies on the Staphylococcal agr Quorum Sensing Circuit
… based on nanometer-scale lipid-bilayer discs (nanodiscs). Activation of this receptor by its native activator, for instance, requires a membrane environment enriched of anionic lipids mimicking the electrostatic property of the S. aureus cell membrane. This strong dependence on lipid …
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Cytochrome P450 2J2 mediated metabolism of omega-6 and omega-3 endocannabinoids
… into the native membrane bilayers of nanodiscs for rigorous spectroscopic and functional studies. Notably, all N-terminal constructs were stably bound within the nanodisc bilayers, which prompted efforts to elucidate additional motifs that impart membrane binding. These observations …
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Hybrids Based on Layered Silicates
… % at 5% filler content. Further, patchy hybrid nanodiscs based on natural montmorillonite as core and a shell made from compartments of two different polymers were evaluated as cheap and versatile compatibilizers in an immiscible polymer blend. In a simple one-step modification process a shell …
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Omics approach towards understanding the function of bioactive lipids in osteosarcoma
… the membrane proteome of osteosarcoma cells in nanodiscs (chapter 3). Nanodiscs are lipid bilayers surrounded by membrane scaffold protein and provide the amphipathic environment for membrane proteins. In this study we showed that lipid composition affects the protein composition incorporated. …
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Resolving Signal Transduction in Complex Biological Environments
… By isolating full-length receptors using nanodiscs produced with cell-free expression, we discovered ligand-induced conformational coupling between the EGFR extracellular and intracellular domain using single-molecule Förster Resonance Energy Transfer measurements. Furthermore, we …
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Towards atomistic characterization of blood coagulation proteins by NMR spectroscopy
… have been incorporated into both phospholipid Nanodiscs and POPC/DPPC liposomes. The impact of different lipid preparations on the structure of TF as well as mTF functional assay data are presented. Our preliminary results indicate that TF retains its primarily beta-sheet secondary structure …
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Global deconvolution of heterotropic cooperativity in cytochrome P450 3A4
… work in our laboratory to isolate the enzyme in Nanodiscs. This provides a homogenous, monodisperse, native-like environment, where the monomeric enzyme is biophysically characterized in the absence of detergent micellar mixtures or liposomal systems which are reported to lead to enzyme …
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