Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"NanoString"”.
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Probabilistic Dynamical Modelling of Spatiotemporal Cell Trajectories During Neural Development
… and cell type mapping methods for the Nanostring WTA spatial transcriptomics technology that take into account, background binding of RNA probes. I use CountCorrect to analyze a spatial transcriptomics dataset of the human developing cortex, which revealed spatial autism enrichment …
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TGFβ signalling in microglia and the impact of microglia-specific Smad4 deletion
… gene expression profiling techniques, such as Nanostring nCounter technology, were utilized to examine microglia-specific gene expression during development and under LPS and TGFβ signalling inhibition. The epigenetic basis for microglia-specific gene expression during early postnatal …
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The perioperative setting as a platform for breast cancer biomarker development
… analysis of baseline archived tumour samples. NanoString codesets BC360 and IO360 were used to compare gene expression in baseline tumours that underwent pCR versus no pCR. NanoString GeoMx digital spatial profiling (DSP) assessed immune protein abundance in intraepithelial and stromal …
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EVALUATION OF THE HEPATIC MICROENVIRONMENT BEFORE AND AFTER ANTIVIRAL THERAPY FOR CHRONIC HEPATITIS C
… treatment. Liver biopsies were analyzed using NanoString nCounter and multispectral imaging to determine gene expression and immune cells phenotypes. Then, the findings were correlated and analyzed with clinical outcomes. Patients achieved sustained virologic response (SVR) post-treatment, with …
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Profiling neuroinflammation in amyotrophic lateral sclerosis
… motor cortex tissue was further profiled using a NanoString nCounter neuroinflammation panel, with findings validated in sporadic ALS-FTSD (n = 18) and SOD1-ALS (n = 5) tissue and an independent public dataset. Further interrogation of candidate pathways and their roles in heterogeneity and cell …
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Spatial transcriptomic analysis of low-grade intestinal T-cell lymphoma and lymphoplasmacytic inflammatory bowel disease in cats
… The spatial transcriptomics technique (GeoMx, Nanostring-Bruker) was successfully performed in T and B lymphocytes of cat samples for the first time, to our knowledge. The samples were assessed according to the diagnosis and compared among three different histologic areas: superficial …
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Integrative Cancer Immunogenomic Analysis of Serial Melanoma Biopsies Reveals Correlates of Response and Resistance to Sequential Ctla-4 and Pd-1 Blockade Treatment
… profiling (12 marker immunohistochemistry and NanoString Gene Expression Profiling), we found that adaptive immune signatures in tumor biopsies obtained from early on-treatment time points are predictive of response to immune checkpoint blockade. We also demonstrated differential mechanistic …
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Analisi dei profili di espressione dei microRNA: identificazione di biomarker nel suicidio
… measured using nCounter® miRNA expression Assay (NanoString Technologies). In total, 28 miRNAs were differentially expressed or regulated by lithium between the two groups at a false discovery rate of 0.1. Two miRNAs were validated with quantitative Real Time-PCR (qRT-PCR): miR-4286, up-regulated …
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Characterisation of a Novel GABA Signalling System in the Human Cerebral Vasculature
… subunits and potentially GABA transporters. NanoString nCounter analysis was utilised to confirm the gene expression of GABAA and GABAB receptor subunits by cerebrovascular cells in vitro, and protein expression was confirmed using Western blot. An immunohistochemical study also revealed …
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PROGRAMMED DEATH LIGAND 1 (PD-L1) IMMUNE CHECKPOINT-MEDIATED STIMULATION OF MALIGNANT PHENOTYPES IN TUMOUR CELLS
… oncogenic signaling and autophagy. Furthermore, Nanostring analysis of MDA-MB-231 cells treated with recombinant PD-1 (rPD-1) revealed a potential role for NOTCH1, WNT, MAPK and TGF-/SMAD signaling downstream of PD-L1. Since autophagy is a well-established mechanism of chemoresistance in cancer …
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The Gut Microbiome as a Novel Approach for Reversing Long-Term Inflammation and Accelerated Aging Across the Lifespan
… by flow cytometry and transcriptomics (NanoString). 16S rRNA sequencing was used for bacterial analysis of fecal samples and validating successful fecal microbiota transplantation (FMT). Mass spectrometry metabolomics measured alterations in tryptophan pathways.</p> …
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Profiling mucinous ovarian cancer and comparisons with tumours of the gastro-intestinal tract: identification of markers for diagnosis, prognosis, and treatment targets.
… colorectal and appendiceal tumours (n=230); a NanoString mRNA gene expression profiling study of mucinous ovarian borderline tumours (n=151), primary MOC (n=333), and upper (pancreatic/gastric, n=65)) and lower (colorectal/appendiceal, n=55) tumours to look for prognostic and diagnostic …
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Tumour infiltrating T cells in non-small cell lung cancer and their interactions with cancer-associated fibroblasts
… In the second results chapter, bulk RNASeq (Nanostring nCounter) was used to assess the phenotype of T cells isolated from NCL and the TME, and the effect of co-culture with CAFs. This revealed that coculture with CAFs mirrored some transcriptome changes seen in T cells isolated from the TME. …
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Profiling of circulating microRNAs in body fluids from Autism Spectrum Disorder patients
… alterations in ASD. Therefore, we also used NanoString nCounter technology to profile supernatant saliva circulating miRNAs from ASD patients and NCs. Through validation assays, we demonstrated that both miR-29a-3p and miR-141-3p are upregulated in ASD saliva compared to NC one. We observed …
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Predicting Response to Neoadjuvant Chemotherapy in Breast Cancer with Gene Expression and Computational Pathology
… available FFPE tissue, using a user-friendly NanoString technique. Unfortunately, it was not possible to achieve reliable classification with this method using the gene probe set pre-selected based on fresh frozen tissue. Next, I sought to identify the genes whose expression was not affected …
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Mechanical High-Intensity Focused Ultrasound (Histotripsy) in Dogs with Spontaneously Occurring Soft Tissue Sarcomas
… (TME) gene expression was evaluated with the Nanostring Canine IO panel, and the systemic immune response was evaluated using multiplex serum cytokine levels. Results: Ten dogs were recruited and treated. Tumor histologies included 3 grade III STS, 4 grade II STS, 2 grade I STS, and 1 …
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Propranolol Elicits Long Term Systemic Effects After Repetitive Mild Traumatic Brain Injury
There are almost 2 million new traumatic brain injuries (TBIs) every year in the US. Of these, 80% of these can be classified as mild TBI, also known as concussions, that can lead to pronounced long term symptoms months and years after injury. The presence of post traumatic headaches (PTH) is the …
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The role of the immune system in Parkinson’s Disease: from neuropathology to treatment.
… region of neurodegeneration in PD). Using two Nanostring nCounter panels for neuroinflammation and glial profiling (totalling ~1300 genes), I found regional differences in the immune signatures of the PD brain compared to controls, with the strongest inflammatory signature seen in the amygdala …
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Pharmacogenomics of sickle cell disease therapeutics: pain and drug metabolism associated gene variants and hydroxyurea-induced post-transcriptional expression of miRNAs
… College of Medicine, United States, using the NanoString Platform (NanoString Technologies, Inc., Seattle, WA, United States), according to manufacturer's instructions. Genes with statistically significant changes in expression were analysed using the significance analyses of microarrays (SAM) …