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Showing 1 to 13 of 13 for “"NanoSIMS"”.
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Correlated NanoSIMS, TEM, and XANES Studies of Presolar Grains
… via Nano-scale Secondary Ion Mass Spectrometry (NanoSIMS), Transmission Electron Microscopy (TEM), and Scanning Transmission X-ray Microscopy (STXM) utilizing X-ray Absorption Near Edge Structure (XANES), with a focus on connecting these correlated laboratory studies to astrophysical phenomena. …
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Applying secondary ion mass spectrometry to cellular and model membranes to image component distribution and quantify composition
… using high-resolution SIMS performed with a NanoSIMS. Further, the spatial statistical analysis tools are outlined and applied to NanoSIMS data to test the hypothesis of colocalization of the flu virus protein hemagglutinin and sphingolipids. While the NanoSIMS has very high lateral …
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Secondary ion mass spectrometry as a tool to evaluate chemical composition within model and cellular membranes
… high-resolution SIMS, performed on a Cameca NanoSIMS 50, and atomic force microscopy (AFM) the influence of cholesterol on the phase behavior of supported lipid membranes containing saturated phosphatidylcholine lipid species was studied. While the NanoSIMS 50 afforded unprecedented lateral …
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Probing the formation of mammalian cell lipid microdomains with nanometer-scale imaging secondary ion mass spectrometry
… imaging secondary ion mass spectrometer (NanoSIMS 50, Cameca), we have been able to chemically map the distributions of sphingolipids on the plasma membrane of murine fibroblast cells with an astonishing sub-100 nm resolution. This required us to metabolically incorporate a rare, stable …
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Deportment of radionuclides in copper concentrate from Olympic Dam
… nanoscale secondary ion mass spectrometry (nanoSIMS) platform, capable of detecting (for the first time) extremely low RN signals in minerals with sub-micron resolution; consequently, nanoSIMS was chosen as the primary analytical method for this project. Subsequent to extensive method …
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Liverwort-Fungal Associations: Novel Symbioses In Polar Regions?
… labelling experiment was established, with NanoSIMS being utilized to visualise the flow of N into liverwort tissue. I found that fungal colonisation was frequent in the liverworts surveyed, with species from the Serendipitaceae being among the most abundant fungal taxa recorded. …
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The History of Water and Other Associated Volatiles in Howardite-Eucrite-Diogenite Meteorites
… System history. <br></br><br></br> Using the NanoSIMS 50L, six eucrites were analysed for their apatite H<sub>2</sub>O abundance and hydrogen isotopes, whilst seven eucrites (five of which were also investigated for H) were analysed for their apatite Cl content and isotopic composition. …
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Mass spectrometry methods for imaging cellular lipids, organelles, and proteins
… ions, such as that employed by the Cameca NanoSIMS 50, necessitate labeling the biomolecule of interest with a distinct atom, such as a rare stable isotope, to allow for distinction of its secondary ions from those of other cellular species. Mass analyzers, the second component of mass …
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Isotopic and Elemental Compositions of Stardust and Protosolar Dust Grains in Primitive Meteorites
… and N isotopic imaging, respectively, using the NanoSIMS 50. Subsequently, the elemental compositions of the grains carrying the anomalous isotopic signatures were acquired in the PHI 700 Auger Nanoprobe. Some silicate grains with unique O isotopic compositions were measured for Si and Fe …
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Using imaging secondary ion mass spectrometry to determine mammalian plasma membrane component distribution and cell differentiation state
… secondary ion mass spectrometry instrument (NanoSIMS 50, Cameca), we have chemically mapped the distributions of labeled sphingolipids and cholesterol within the plasma membrane of murine fibroblasts with sub-100 nm resolution. This required the incorporation of non-perturbing heavy isotope …
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Development and application of tools to visualize and assess the roles of specific biomolecules in cells
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-08-01
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Tools for imaging intracellular lipid distributions with high-resolution secondary ion mass spectrometry
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-08-01
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Multi-element isotopic analyses of presolar graphite grains from the Orgueil meteorite
This dissertation presents the results and implications of the isotopic analyses of presolar graphite grains from the primitive carbonaceous chondrite, Orgueil. Graphite grains from low- and high-density fractions were analyzed for C, N, O, Si, Al-Mg, K, Ca, and Ti isotopes. These analyses indicate …