Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"Naïve pluripotency"”.
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Instructing naïve pluripotency ; JAK/STAT3 and Nanog in reprogramming
Reprogramming of somatic cells to pluripotency is a topic of extensive research driven by the potential of induced pluripotent cells in in vitro disease modelling, drug screening and regenerative medicine. The pluripotency associated transcription factor Nanog has been subject to a lot of …
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The NuRD complex regulates chromatin dynamics driving exit from naïve pluripotency
… (NuRD) complex is essential for exit from naive pluripotency both in mammalian embryonic stem cell (ESC) culture and in vivo. However, the specific mechanisms of chromatin modulation by NuRD in this process have not been fully characterised. This study used rapid, auxin-inducible removal of NuRD …
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Mechanistic Dissection of Tcf7l1-Dependent Enhancer Decommissioning During Exit from Naïve Pluripotency
The transition out of naïve pluripotency requires the simultaneous dissolution of the naïve gene regulatory network as well as a redirection of transcriptional response to Wnt/β-catenin signaling. Here, we show that Tcf7l1 functions as a linchpin factor that connects the two outcomes via effects at …
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The role of SOCS3 in nuclear reprogramming and naïve pluripotency network integration
… functional commitment of the cell is known as pluripotency. Pluripotency is the ability of a cell to give rise to the derivatives of the three germ layers and germ cells during differentiation. Naturally, pluripotency is restricted to the early embryo only and represented by two …
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Studying the principles of cell identity transitions using naïve pluripotency induction as a model
… on reprogramming from epiblast stem cells to naïve pluripotency. By independent modulation of genetic and signalling parameters followed by isolation of successfully transitioning cells, I showed that naïve pluripotency can be established via distinct routes. These differ in their …
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A study into the biology of mESCs exiting naïve pluripotency and technology development for subcellular proteomics
… embryology for decades. One of those aspects is pluripotency, the ability of embryonic cells of certain developmental stages to divide indefinitely without the loss of their potential to differentiate into derivatives of the three primary germ layers. Pluripotency has been captured in vitro by …
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Elucidating the Role of Plakoglobin in Embryonic Stem Cell Culture
Pluripotency – the ability of cells to differentiate into all three germ layers – is of great interest, particularly in the fields of developmental biology and medicine. The in vivo continuum can be separated into three in vitro states that are also found in the embryo: naïve, formative, and primed …
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Determining the Signalling Pathways that Govern Human Naive Pluripotency
… and occupy a developmentally advanced state of pluripotency comparable to mouse EpiSCs. Recent reports demonstrate that a naïve state of human pluripotency can be consistently derived either through transient histone deacetylase inhibition mediated resetting of conventional hESCs or via …
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A Nuclear Mechanoresponse to Morphogenesis Drives Acquisition of Neuroectoderm Lineage Competence During Pluripotent Cell Differentiation
… differentiation. I therefore hypothesized that pluripotency and subsequent cell fate specification involves nuclear mechanosensitivity to morphogenesis-associated forces. To test this, I investigated the role of actomyosin contractility and the LINC complex in a model of exit from naïve …
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Identification of the ERK5 Kinase as a Key Regulator of Embryonic Stem Cell Pluripotency
… types in the adult body, a phenomenon known as pluripotency. Distinct pluripotent states have emerged as a key concept in ESC biology; the naïve state safeguards selfrenewal whilst primed pluripotency provides a window for differentiation. However, the molecular mechanisms that control …
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A Study of Mouse Peri-Implantation Morphogenesis
… surrounding a central lumen while exiting from naïve pluripotency. I specifically concentrated on the role of Integrin signalling during these stages taking advantage of Integrin β1 deficient mouse embryonic stem cells (mESCs) (3). Subsequently, I decided to shed light into the dynamics of …
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The Regulation of UHRF1 during Mammalian Embryogenesis
… the regulation of DNA methylation machinery in naïve pluripotency highlights that proteasomal degradation of UHRF1 driven specifically by ERK inhibition is the principal cause of global demethylation in these conditions. Mass spectrometry analysis of post-translational UHRF1 phosphorylations as …
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The Role of p66Shc in Stem Cells: Signalling, Identity and Apoptosis
… serine-727 phosphorylation, thereby sustaining naïve pluripotency even in the absence of chemical MEK/GSK3β inhibition. These effects are consistent with a model in which p66Shc acts as a competitive inhibitor of Grb2 recruitment, restraining ERK–STAT3 signalling to promote lineage priming. In …
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Interrogations of protein and RNA: localisation, interactions and dynamics
… induced stress response and stem cell pluripotency phase transition. In Chapter 3, a one-step subcellular fractionation procedure using multiple iodixanol containing gradients, termed “MultiLOPIT”, is presented, which provides high-resolution organelle resolution for comparative …
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Mechanisms of Oct4 in the entry to, maintenance of, and exit from pluripotency
Pluripotency is defined as the capacity to give rise to all cell types of the embryo proper. It arises in the early mammalian embryo but is lost after a short period of time as cells differentiate and become committed to different lineages. Prior to implantation, mouse epiblast cells enter the …
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Defining the essential regulators of naive human pluripotent stem cell reprogramming
… stem cells (PSCs) exist in two distinct states – naïve and primed, which are interconvertible. Naïve PSCs uniquely harbour several desirable properties that primed PSCs do not, including their ability to model human pre-implantation biology and developmentally-regulated epigenetic phenomena in …
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Defining the minimal and context-dependent signalling cascades regulating the maintenance of human naïve embryonic stem cells and early embryo development
Human naïve embryonic stem (HNES) cells can self-renew under defined culture conditions and closely resemble the preimplantation epiblast lineage. Whilst some of the signalling cascades that are fundamentally implicated in modulating the naïve gene regulatory network have been studied, our …
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Pluripotency and patterning in nonhuman primate embryogenesis
… to unravel primate-specific features of pluripotency. This thesis examines common marmoset (Callithrix jacchus) development to explore the molecular cues that govern (1) naïve pluripotency in the preimplantation epiblast, and (2) primed pluripotency in the embryonic disc (EmDisc) in the …
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CHARACTERISING THE REPROGRAMMING DYNAMICS BETWEEN HUMAN PLURIPOTENT STATES
… stem cells (hPSCs) exist in multiple states of pluripotency, broadly categorised as naïve and primed states. These provide an important model to investigate the earliest stages of human embryonic development. Naïve cells can be obtained through primed-to-naïve reprogramming; however, there are …