Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"NXF1"”.
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Structural characterization of a protein/RNA complex : human TAP/NXF1 protein/retroviral CTE RNA
… particles (mRNPs). In humans, the protein TAP/NXF1 interacts with mRNA export cargoes and mediates their nuclear export by shuttling through nuclear pore complexes (NPCs). TAP is a multidomain modular protein composed of an N-terminal mRNP-binding region and a C-terminal NPC-binding region. TAP …
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Tap /Nxf1 N -Terminal Domain Mediates Homotypic Complex Assembly and Is Necessary for Nuclear RNA Export
Tap/NXF1 is the metazoan nuclear export receptor for both poly (A+) RNA as well as cellular and viral RNAs containing the constitutive transport element (CTE). Tap is a multi-domain protein that interacts directly with nucleoporin proteins of Nuclear Pore Complexes (NPCs) as well as messenger RNA …
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Characterization of Nuclear Localization Signals of Karyopherin-Mediated Nuclear Import
… Unexpected redundant import pathways for NXF1 are also discovered using M9M peptide. The N-terminal disordered region of human NXF1 contains NLSs for Imp beta, Kap beta2, Imp4, Imp11 and Imp alpha. Mutation of the NLSs in NXF1 abolished binding to the Karyopherins, mislocalized NXF1 to the …
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The role of human and Drosophila NXF proteins in nuclear mRNA export
… of the four predicted Drosophila NXF members (NXF1-3) were found to be expressed in this cell line and could be targeted by RNAi. Depletion of endogenous NXF1 inhibited growth and resulted in the nuclear accumulation of polyadenylated RNA. Fluorescence in situ hybridization revealed that export …
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The Influenza A Virus NS1 Protein and Vriral mRNA Nuclear Export
… are exported is poorly understood. The cellular NXF1-dependent mRNA export pathway has been shown to be involved in the export of some viral mRNAs, but how they are recruited to this pathway is unknown. Prior work from our laboratory showed that segment 7 mRNA was inefficiently exported to the …
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Investigating upstream regulators of FOXA1 in breast cancer
… screens identified several regulators, including NXF1, a nuclear RNA export factor. RNAscope analysis showed that NXF1 controls FOXA1 protein levels via regulation of FOXA1 mRNA nuclear export. Mechanistic experiments further revealed that NXF1 associates with nascent RNA co-transcriptionally on …
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The Role of NS1-BP in Influenza Virus Replication
… shown that NS1 binds an mRNA complex containing NXF1/TAP, NXT/p15, Rae1, and E1B-AP5, which are key components of the mRNA export machinery. By targeting this complex, NS1 blocks host mRNA export, and cells become highly permissive to viral replication. Another intranuclear pool of NS1 was found …
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Nuclear binding and translation regulation by FMRP through novel associated protein and putative RNA helicase MOV10
… FMRP enters the nucleus and that it requires Tap/Nxf1 for efficient export from the nucleus. Additionally, I use the Xenopus laevis oocyte system to demonstrate that FMRP is indeed present along nascent mRNA transcripts suggesting that FMRP enters the nucleus and can target newly transcribed RNAs. …