Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"NUCLEOLI"”.
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Nucleolar dynamics in cellular senescence
… gene expression associated with enlarged nucleoli is not well understood. Here, I show nucleolar dynamics in different types of cellular senescence including oncogene-induced senescence (OIS), secondary senescence, and replicative senescence (RS). I have used human diploid fibroblast that …
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The Nucleolar Protein Nucleophosmin Undergoes Liquid-Liquid Phase Separation with Arginine-Rich Nucleolar Proteins through Weak, Multivalent Electrostatic Interactions
Nucleoli are nuclear membrane-less organelles that are the sites for ribosome biogenesis and serve as sensors of cellular stress. Weak, multivalent protein-protein interactions and interactions between disordered, low complexity domains (LCDs) and rRNA have been shown to promote liquid-liquid phase …
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Lokalisation und Dynamik der Replikationsproteine des murinen prä-replikativen Komplexes
… Centrosom lokalisiert waren konnte ORC6-EGFP in Nucleoli nachgewiesen werden. Die EGFP-Fusionsproteine von Cdc45p, PCNA und DNA-Ligase-I waren im Zellkern lokalisiert, die Nucleoli waren ausgespart. Ein weiterer Aspekt dieser Arbeit war die Untersuchung der Substratspezifität der murinen …
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Dynamics of human DNA Topoisomerases I and II
… We show that this is due to the fact that in the nucleoli they are moving slower than in the nucleoplasm. The decreased nucleolar mobility cannot be due to DNA-interactions, because compounds that fix topoisomerases to the DNA deplete them from the nucleoli. Interestingly, the subnucleolar …
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The post-translational methylation of arginine in the glycine arginine rich region of CHO nucleoin
Nucleolin is a nucleolar protein important for ribosome biogenesis. Nucleolin contains a conserved glycine arginine rich (GAR) domain near its carboxy terminus. GAR domains are defined by repeating tri-peptide, (arginine -glycine-glycine (RGG)) motifs. The arginine in the RGG motif is …
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Distinguishing the Treefrogs "Hyla versicolor" and "Hyla chrysoscelis" in Iowa, and Their Distributions
… separation of the species by counts of stained nucleoli and measurement of scanning electron micrographs of toepad projections. The resulting distribution correlates with spot checks of call analysis. Subsequent morphometric data confirm that the two species represent two distinct populations in …
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Characterization of a novel family of nucleolus localized RNAs
… sub-unit assembly take place. In human cells, nucleoli are formed around nucleolar organizer regions (NORs) located on the short arms of the five acrocentric chromosomes. NORs are composed of tandem copies of hundreds of rDNA repeats. However, the sequence beyond the rDNA array within the …
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Human Neuroblastoma, Strain Sk-N-Sh, as a Model System of Nerve Cell Differentiation: Neuronal Development, Plasminogen Activator Release, and Unscheduled Deoxyribonucleic Acid Synthesis
… exhibited a large nucleus, with several distinct nucleoli, and neurite- and dendrite-like processes with branched end terminals. Within a few hours after subcultivation, the morphological complexity of the cells increased as evidenced by a greater number of cells with neurite processes longer than …
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Low complexity regions in Biological Matter: Sequences, higher-order assembly, and evolution.
From the fibrous silk of a spider to liquid-like nucleoli, biological matter plays important roles in organisms. How does biological matter assemble from component parts, carry out important organismal functions, and evolve in the tree of life? Low complexity regions (LCRs) of proteins are simple …
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Genetic and cytological investigation of Nucleostemin-1 in Drosophila melanogaster
… ATPase activity, and that it is present within nucleoli of most larval and adult cells. Endogenous NS1 and lightly expressed GFP-NS1 enrich within the nucleolar granular regions as expected, while over-expressed GFP-NS1 localizes throughout the nucleolus and nucleoplasm, and to several …
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Exploring the link between RAG1 Cellular Distribution and Components of the CRL4DCAF1 E3 Ligase
… and the 26S proteasome. RAG1 sequestration in nucleoli and nuclear puncta have been implicated in regulating its activity. RAG1 nucleolar egress and puncta formation are dependent on the same domain as DCAF1 association. We investigated whether DCAF1 or the CRL4 ligase is responsible for …
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Histology of bovine mammary tissue during advanced stages of induced lactation
… had spherically shaped nuclei containing several nucleoli and a decreased nuclear/cytoplasmic ratio when compared to day 18 epithelia. Four of the hormonally treated cows were allowed to lactate. Production from these four cows ranged from 33 to 74% of the previous lactation. Production and …
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Human FRG1 is an Actin-Bundling and a RNA-Associated Protein with Distinct Subcellular Localizations
… fraction of the endogenous FRG1 is localized in nucleoli, Cajal bodies, and actively transcribed chromatin associated with nascent RNA transcripts, supporting a function in RNA biogenesis. Furthermore, we show that FRG1 interacts specifically with RNA in vitro, associates with mRNA in vivo, and …
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Site-specific phosphorylation of histone H1 associated with cell cycle progression and transcription
… nuclei but appear to be particularly enriched in nucleoli. Chromatin immunoprecipitation analyses reveal that interphase phosphorylated H1.4 is enriched at active rDNA promoters and is rapidly induced at steroid hormone response elements by hormone treatment. Our results imply that site-specific …
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Transplantation of the Mammalian Pineal Gland: Studies of Survival, Revascularization, Reinnervation and Recovery of Function
… by the presence of euchromatic nuclei, prominent nucleoli, infolded nuclear envelopes, numerous mitochondria and Golgi apparati, and relatively abundant endoplasmic reticulum and ribosomes. In addition, morphological correlates of two putative secretory processes were observed in the grafts. The …
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Nucleolar Stress Due to Depletion of Nopp140 in Drosophila melanogaster
… instar stage and died within 8 days. While nucleoli appeared intact in Nopp140-/- cells, the C/D snoRNP methyl-transferase, fibrillarin, redistributed to the nucleoplasm in variable amounts; RT-PCRs showed that 2’-O-methylation of rRNA in Nopp140-/- cells was reduced. Ultra structural …
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Immunotoxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and Diethylstilbestrol (DES) in the Fetal Mouse Thymus and Liver
… cytoplasmic basophilia and more prominent nucleoli. Real time quantitative PCR demonstrated that DES decreased c-jun, Bcl-2, and PKCalpha mRNA expression. These results suggest a shift away from proliferative activity and may reflect alterations noted predominantly in the hematopoietic …
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OROPOUCHE VIRUS DISRUPTION OF HOST CELL FUNCTIONS: PYROPTOSIS INDUCTION AND TRANSCRIPTIONAL SUPPRESSION
… research reveal that OROV NSs protein targets nucleoli and suppresses host transcription by reducing the levels of the C-terminal domain of the RPB1 subunit of RNA Polymerase II (RNAPII), primarily the hyperphosphorylated llo form. Wild-type (wt) OROV infection decreases RPB1 independently of …
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Genomic and microscopic dissection of nuclear compartmentalization and function
… in the nuclear interior, specifically at nucleoli and nuclear speckles. But it is still elusive how such nuclear compartments (bodies) are involved in genome organization. Nuclear speckles are a type of nuclear body enriched in RNA metabolic factors and correlated with active gene …
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Understanding T cells in type 1 diabetes: a role for c-Maf and characterization of intracellular signaling following engagement of transgenic Ly49A.
… leukemia nuclear bodies (PML-NBs) or nucleoli, respectively. Sumoylation of c-Maf and phosphorylation of p65 are increased in NOD CD4 cells compared to CD4 cells from diabetes-resistant B10.D2 mice, suggesting that increased c-Maf sumoylation and interaction with p65 contribute to …
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