Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 102 for “"NUCLEASES"”.
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Optimizing Zinc Finger Nucleases for Use in Mammalian Cells
… break within the target locus. Zinc Finger Nucleases (ZFNs) are designed proteins that fuse a zinc finger DNA binding domain to the nuclease domain from the FokI restriction endonuclease and have been used to induce double strand breaks at precise sequences. Although ZFNs have been …
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A eukaryotic platform for the enhancement of Cas nucleases
… however unbiased screenings to enhance Cas nucleases have been described only in bacterial cells. Here we develop a fully eukaryotic platform to evolve inefficient Cas proteins toward highly active nucleases. A first step of directed evolution is performed in yeast to isolate enhanced …
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Genetic Correction of Duchenne Muscular Dystrophy using Engineered Nucleases
… the native dystrophin gene using engineered nucleases that target one or more exons in a mutational hotspot in exons 45-55 of the dystrophin gene. Importantly, this hotspot mutational region collectively represents approximately 62% of all DMD mutations. In this work, we utilize various …
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Functional Roles of Nucleases in DNA Metabolism and Genome Stability
… DNA repair pathways that require the action of nucleases, specifically Exonuclease 5 and Ribonuclease H2. First, I describe the biochemical and molecular characterization of the novel Exonuclease 5 family of enzymes from S. cerevisiae, S. pombe, and humans. The Exo5 family consists of …
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Engineering programmable nucleases for in vivo and in vitro applications
Programmable nucleases are powerful molecular tools that have significantly advanced biotechnology development. Enzymes such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), clustered regularly interspaced short palindromic repeats (CRISPR) associated …
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Accessory Nucleases Provide Robust Antiparasite Immunity for Type III CRISPR-Cas Systems
… genome through complementary base pairing. Cas nucleases then destroy the invader's genome. To date, six major types and multiple subtypes of CRISPR systems exist, each with their own signature genes and mechanisms of action. Type III CRISPR systems are uniquely able to destroy both the …
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Engineering of transcription activator-like effector nucleases (TALENs) for targeted genome editing
… transcription activator-like effector (TALE) nucleases (TALENs) as an efficient tool for targeted genome editing. Targeted genome engineering relies on the introduction of a site-specific double-strand break (DSB) in a pre-determined genomic locus by a rare-cutting DNA endonuclease. Subsequent …
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Roles of non-Cas nucleases in Type III-A CRISPR-Cas immunity
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-08-01
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In Vitro Selection of Highly Efficient DNA Enzymes as RNA Nucleases and Metal Biosensors
Based on the superior activity of 17E in Pb2+ than in other metal ions, we evaluated its potential application in sensing Pb 2+ ions. Combining the high selectivity of 17E towards Pb2+ and the high sensitivity of fluorescence detection, 17E can measure the presence of Pb2+ ions over a concentration …
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Regulation of structure-selective nucleases by the SLX4 scaffold in ICL repair and HJ resolution
… includes three different structure-selective endonucleases: XPF-ERCC1, MUS81-EME1 and SLX1. The SLX4 complex has been shown to have essential roles in repair of DNA interstrand crosslinks (ICLs). In addition to its role in ICL repair, the SLX4 complex has the relatively unusual property of being …
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The Design and Development of Artificial Zinc Finger Transcription Factors and Zinc Finger Nucleases to the hTERT Locus
… transcription factors (ZFTFs) and zinc finger nucleases (ZFNs) to target sequences at the hTERT promoter. We first explored expansions to the repertoire of sites that can be targeted by ZFNs and modifications of ZFN architecture to accommodate such sites. A ZFN is made of a zinc-finger DNA …
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ELECTROPHORETIC AND ENZYMATIC IDENTIFICATION OF PROTEINS ASSOCIATED WITH THE DEVELOPMENT OF COMPETENCE IN BACILLUS SUBTILIS
… zymogram procedure for the analysis of nucleases is described. Using purified deoxyribonuclease I (bovine pancreas), as little as 10 pg of nuclease can be detected. Isoelectric focusing (IEF) and non-equilibrium pH gradient electrophoresis (NEPHGE) were compared as first dimensions, in …
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Development of a Yeast Transcription System Using a Saccharomyces Cerevisiae Enzyme Extract and a Drosophila Melanogaster Histidine tRNA Gene
… for the detection of S. pombe processing nucleases. The synthesized [? – 32p] labeled precursor tRNAs were purified using polyacrylamide gel electrophoresis. Autoradiographic techniques were employed to identify the location of the precursor on the gel. The precursor was then eluted from …
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Mitochondrial genome engineering in the murine germline using designer nuclease technology
… a dimeric nuclease domain, producing zinc finger nucleases (ZFN) or TALE-nucleases (TALEN), respectively. By targeting these pairs of engineered nucleases adjacent to a site of interest, a DNA double-strand break (DSB) can be produced, leading to degradation of the mtDNA molecule. Using novel …
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Toward the Synthesis of Nuclease Models.
<p>Nucleases are enzymes that can specifically recognize nucleic acids and hydrolyze their phosphodiester bonds effectively. As is the case with many hydrolases, nucleases often carry one or more metal centers. Cooperation between such metal centers and other interactions involving general …
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Využitie transgénnych rastlín pri štúdiu funkcie rastlinných génov
… particular selected gene using site-specific endonucleases, which include meganucleases, zinc finger nucleases, TALEN (transcription activator-like effector nucleases) and CRISPR (clustered regularly interspaced short paindromic repeats). In contrast to inactivation approaches, there are …
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Modelling and correction of retinal pathologies caused by mitochondrial DNA mutations via genetic engineering
… far, most methods have relied on programmable nucleases. The recent emergence of mitochondrial base editors has broadened the scope of mtDNA genetic engineering approaches. However, the potential of programmable nucleases or base editors for modifying retinal mtDNA remains unexplored. This …
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