Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"NSP2"”.
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Structure-guided insights into the functions of CHIKV nsP2
… poolt kodeeritud mitte-struktuurvalgud (nsP-d). nsP2 valku peetakse RNA replikatsiooni võtmevalguks ja peamiseks regulaatoriks. Siiski olid nsP2 valgu ensümaatiliste aktiivsuste ja muude funktsioonide omavahelised seosed ebapiisavalt uuritud. Käesolev uuring selgitas välja nsP2 valgus struktuuri …
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Identification and characterization of a novel structural protein of porcine reproductive and respiratory syndrome virus, the replicase nonstructural protein 2
… consequence. The PRRSV non-structural protein 2 (nsp2) is a large multifunctional protein encoded by the most genetically diverse region of the genome - the selective pressure potentiating mutation within this region is unknown. Here we report the identification of a unique function of nsp2 as a …
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The roles of papain-like protease-related proteins in viral replication and host immunity
… are highly required in swine industry. PRRSV nsp2-related proteins including nsp2 and two ribosomal frameshifting products-nsp2TF and nsp2N share the same N-terminal PLP2 domain. In chapter 2, nsp2TF and nsp2N were demonstrated to be critical for host innate immunity suppression at least …
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Phase Separation Mediated Compartmentalisation of Rotavirus Replication
… disordered protein NSP5 binds the RNA chaperone NSP2, viral RNAs and other viral proteins to form replication factories known as viroplasms. Early in infection, these conden- sates are liquid-like and dynamic, whereas at later stages they undergo maturation that coincides with …
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Reverse Genetics-based Approaches to Attenuate Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)
… identified a spontaneous 435-bp deletion in the nsp2 gene. To characterize the biological and pathological significance of this nsp2 deletion, we restored deleted nsp2 sequence back to pIR-VR2385-CA and constructed another clone pIR-VR2385-R. VR2385-CA and VR2385-R were successfully rescued in …
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Análises estruturais e de modelos de interação das proteases NS3 dos Flavivirus zika e dengue, e nsP2 dos Alphavirus chikungunya e mayaro utilizando ferramentas in silico e in vitro
… alvos. Estas proteínas (NS3 nos Flavivirus e nsP2 nos Alphavirus) possuem a função de clivar determinadas regiões da poliproteína viral sendo essenciais para a replicação. O objetivo do presente trabalho é realizar análises in silico e in vitro das proteases dos arbovírus ZIKV, DENV, CHIK e …
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Studies on the interaction between porcine reproductive and respiratory syndrome virus and its natural host cell
… been associated with the activity of arterivirus nsp2 and its coronavirus equivalent, nsp3. Moreover, in our previous study, the arterivirus, porcine reproductive and respiratory syndrome virus (PRRSV) was shown to be incapable of inducing type I IFN synthesis upon infecting porcine alveolar …
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Investigating the dynamic nature of biomolecular condensates that support rotavirus replication
… acts as a scaffold, along with the RNA chaperone NSP2 as the major client biomolecule. While viroplasms have been recognised as ribonucleoprotein condensates that serve as replication sites for RVA, many aspects of their formation, functions, and composition remain poorly understood and require …
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Examination of Antiviral Resistance in Venezuelan Equine Encephalitis Virus
… and its derivatives through mutations in the nsP2 and nsP4 genes, but the number, timing of emergence, and the extent of penetrance of these SNPs depend on the compound. These mutations emerged more slowly when infecting an astrocyte cell line. We also found that neurons in the mouse brain did …
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Analysis of Medicago truncatula transcription factors involved in the arbuscular mycorrhizal symbiosis
… MtGRAS8 seems to form heterodimers with NSP2 and RAM1, which are known to be key regulators of the fungal colonization process (Hirsch et al., 2009; Gobbato et al., 2012). These data indicate that MtGras8 and miRNA5204* are linked to the sym pathway and regulate the arbuscule development …
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Ribosome profiling of porcine reproductive and respiratory syndrome virus reveals novel features of viral gene expression
… mechanism, generating alternative forms (nsp2N and nsp2TF) of a viral non-structural protein, nsp2. Frameshift efficiency at both sites was quantified and, at the non-canonical site, was found to increase with time, rendering this the second known example of temporally regulated PRF during …