Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 40 for “"NS3"”.
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Spacecraft-ns3: Spacecraft Discrete-Event Network Simulation
… software. This research presents Spacecraft-ns3, an extension to the ns-3 network simulator. Using a modular approach, Spacecraft-ns3 propagates orbit state, plans discrete events, and analyzes network metrics and flows. A case study using Spacecraft-ns3 is presented for exploratory space …
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Hepatitis C Virus NS3/4A Protease and the Intracellular Antiviral Response: Mapping Complex Virus-Host Interactions
… by the nonstructural 3/4A protein complex (NS3/4A) cleavage of IPS-1. NS3 structural composition includes an amino-terminal serine protease and a carboxy-terminal RNA helicase. A structure-function analysis of NS3/4A truncation and deletion mutations was conducted. Mutants lacking the …
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Control of the Interferon Regulatory Factor - 3 Antiviral Pathway by The Hepatitis C Virus Ns3/4a Protease
… signaling pathways. Our studies identified the NS3/4A protein as an interferon regulatory factor 3 (IRF-3) and NF-<small>K</small>B antagonist and further mapped this activity to the NS3/4A serine protease domain. HCV antagonism of IRF-3 and NF- <small>K</small>B prevents the expression of …
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Control of the Interferon Regulatory Factor - 3 Antiviral Pathway by The Hepatitis C Virus Ns3/4a Protease
… signaling pathways. Our studies identified the NS3/4A protein as an interferon regulatory factor 3 (IRF-3) and NF-<small>K</small>B antagonist and further mapped this activity to the NS3/4A serine protease domain. HCV antagonism of IRF-3 and NF- <small>K</small>B prevents the expression of …
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Studies Toward the Synthesis of Salvinorin A and Synthesis of Broad-Spectrum DENV/WNV NS3 Protease Inhibitors
… features including a highly homologous NS3 protease. NS3 is essential for virion replication, making it an ideal target for a broad-spectrum inhibitor of the related viral proteases.
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Functional analysis of hepatitis C virus non-structural protein (NS) 3 protease and viral cofactor NS4A
… targets is the non-structural (NS) protein 3 (NS3) membrane-bound protease. The major aim of this thesis was characterization of NS3 allosteric activation by its viral cofactor, NS4A. We hypothesized that there would be specific residues that dominate the interaction between NS3 and NS4A, and …
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Expression Of Hepatitis C Viral Non-structural 3 Antigen In Transgenic Chloroplasts
… sequences,human or animal pathogens. The HCV NS3 antigen was expressed in the chloroplast of Nicotiana tabacum var. Petit havana and LAMD-609. The 1.9kb NS3 gene was cloned into a chloroplast expression vector, pLD-Ct containing the 16S rRNA promoter, aadA gene coding for the spectinomycin …
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Characterization and sequence variation of the virulence-associated proteins of different tissue culture isolates of African Horsesickness virus serotype 4
… of viral replication. Nonstructural protein NS3 may play an equally important role due to its function in release of viral particles from cells. Two viruses were obtained for this study, AHSV-4(1) and AHSV-4(13). The thirteenth passage virus, AHSV-4(13), originated from the primary isolate …
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Untersuchung rekombinanter Vakziniaviren MVA auf Eignung als Vektorimpfstoff gegen Infektionen mit dem Hepatitis-C-Virus
… Core, sowie das Nichtstrukturprotein NS3, welches als regulatorisches Virusprotein im HCV-Replikationszyklus eine wichtige Rolle spielt, untersucht werden. Hierfür wurden die rekombinanten MVA-Viren MVA-P7.5-HCV core (MVA-core) und MVA P7.5-HCV-1-830 (MVA-1-830) eingesetzt, welche für …
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Extent of genotype-specific humoral immunity produced on infection with hepatitis C virus: relevance for serological screening and diagnosis
… variation in the antigenic determinants in core, NS3, NS4A/B and NS5A/B proteins, may produce genotype-specific antibodies which do not cross-react with the antigens used in cunent screening assays for detection of antibody to HCV. Reduction in test sensitivity caused by genotype-specific …
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Functional Investigation of RNA Viral Helicases as Molecular Targets for the Discovery of Novel Antiviral Agents
… and of Flaviviruses, particularly the NS3 of WNV. Despite belonging to SF2 and SF1, respectively, the two proteins have strong similarities: both are non-ring forming helicases with core RecA-like domains; they share conserved motifs across the helicases and possess similar NTPase, …
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HCV Resistenztestung
… auf Resistance Associated Mutations (RAM) in der NS3-, NS5A- oder NS5B-Region zurückzuführen. Fragestellung: Die Entwicklung einer Methode zur genotypspezifischen Resistenztestung, die neben Forschungsaspekten vor allem auch für den Einsatz in der virologischen Diagnostik geeignet ist, ist …
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Patterns of Selection In Hepatitis C and Hiv
… In the case of both HCV-1a and HCV-1b, the NS3 protein (with protease and NTPase/helicase activity) demonstrated a high incidence of forward-and-backward or parallel nonsynonymous changes in CTL epitopes. This pattern is best explained by the frequent re-occurrence of the same set of escape …
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Simulation, performance and interference analysis of multi-user visible light communication systems
… the dissertation concerns with developing a new ns3-based VLC module that can be used to study VLC-RF heterogeneous networks via simulation. The proposed ns3 module is developed based on existing models for intensity modulated LED signals operating as lighting units transmitting to optical …
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Feasibility of Ellipsometric Sensor Development for Use During PECVD SiOx Coated Polymer Product Manufacturing
… packaging. Nano Scale Surface Systems, Inc. (NS3), a company which coats polymers with ceramic oxides like SiO2 through a process known as plasma enhanced chemical vapor deposition (PECVD), was interested in the feasibility of an in line measurement system for monitoring the deposited films on …
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Deployment of a next generation networking protocol
… In previous work, initial simulations in NS3 showed that GCN offers high delivery with low network overhead in the presence of high packet loss and high mobility. We extend this prior work to verify GCN's performance in actual over-the-air experimentation. In the experiments, we deployed …
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Natural Killer Cell Responses in Chronic Hepatitis C Virus Infection
… the HCV envelope (E1, E2) and nonstructural (NS3) sequences. Specificity controls consisted of a pool of overlapping hepatitis D virus peptides and samples without peptide addition. As a readout for NK and T cell function, the frequency of IFNγ+ NK cells and T cells was assessed by multicolor …
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The characterisation of aspects related to the single stranded RNA binding ability of African horsesickness virus nonstructural protein NS2
… genes were obtained, the genes encoding NS2, NS3, VP6 and VP7. mRNA transcripts of NS3 and VP7 genes, lacking three to five terminal nucleotides at either the 5' or 3' ends, were also obtained. A nonspecific mRNA control was derived from an AHSV gene cloned in the wrong transcriptional …
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Development of improved methodologies for the discovery of novel bioactive compounds and their application to problems of biomedical relevance.
… studied have been Zika virus protease NS2B-NS3, acetyl and butyrylcholinesterase enzymes, the α-galactosidase enzyme and various proteins involved in diabetes. The first contribution of this thesis (which is presented as a compendium of publications) investigated the discovery of a …
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