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Showing 1 to 20 of 38 for “"NRPS"”.
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Comparative and Functional Genomic Analysis of <i>Streptococcus Equi</i> and <i>Streptococcus Zooepidemicus</i> : Identifying Novel Vaccine Targets
… nonribosomal peptide synthetase (NRPS) system. The NRPS shares similarity with the yersiniabactin system found in the high pathogenicity island of <i>Yersinia pestis</i> and is the first of its kind to be identified in streptococci. As this genetic feature is absent from the <i>S. …
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Reengineering and Discovery of Nonribosomal Peptide Synthetases
… assembled on nonribosomal peptide synthetase (NRPS) assembly lines covalently tethered to the synthetases throughout biosynthesis. Mass spectrometry is an especially well suited tool for analyzing both the products of NRPS pathways and the machinery that produces them. A review of NRPS …
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Biosynthetic characterisation of secondary metabolites from stromatolite cyanobacteria Symploca sp. 1013
… (PKS) or non-ribosomal peptide synthetise (NRPS). Bioactivities in areas such as antibacterial, antifungal and anticancer have been implicated in many of these metabolites. In the present study, various marine cyanobacterial isolates from from stromatolites in Shark Bay, Western Australia, …
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Studies of new natural products and their biosynthesis spearheaded by high-resolution mass spectrometry
… by non-ribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs). In NRPS and PKS biosynthesis, monomer units are incorporated into a growing natural product chain in an assembly line fashion using a covalent thiotemplate mechanism. Because the growing natural product is …
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Effects of Macadamia integrifolia husk substrate and Moringa oleifera leaf extract on macro propagation of banana (Musa spp.) using the Plants Isus de Fragments de tiges (plants issued from stem fragments (PIF) technique
… per shoot (NLPS), number of roots per shoot (NRPS), and long shoot root length (SRL), the first transplanting cycle produced tall suckers with thick pseudo-stem diameter (PSD). A highly significant positive correlation was observed between number of roots per shoot (NRPS) and NLPS as well as …
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Identification and preliminary characterization of the 2,5-diphenyloxazole biosynthetic pathway in streptomyces polyantibioticus SPRT
… whereby a non-ribosomal peptide synthetase (NRPS) condenses a molecule of benzoic acid with 3-hydroxyphenylalanine. The dipeptide is converted to a diphenyloxazole derivative by heterocyclization and a final decarboxylation step leads to DPO. To determine whether the hypothesis pertaining to …
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Detection and further characterisation of the toxins and associated genes of Bacillus cereus
… produced by non-ribosomal peptide synthetases (NRPS) from the genera Bacillus and Streptomyces. Turgay and Marahiel (1994) developed universal primers to detect a 500 base pair (bp) region that has been highly conserved in all of the NRPS genes sequenced thus far. These primers were utilised to …
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Investigation of a novel iron-uptake system and other genomic features in mecC Staphylococcus aureus
… non-ribosomal peptide synthetase (NRPS), directly downstream of the SCCmec insertion site in mecC S. aureus isolates. A homologous region was identified in Streptococcus equi 4047 (S. equi) which encodes a NRPS termed ‘equibactin’ that is involved in iron acquisition. I have …
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Analysis of the diverse antibacterial strategies used by <i>Serratia marcescens</i> Db10
… peptide synthetases (NRPS) and polyketide synthases (PKS) are widespread in bacteria and fungi and are responsible for the biosynthesis of many metabolites with medically relevant properties, including antibiotics. The activity of NRPS and PKS enzymes is dependent on …
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Insights into the Synthesis, Mechanism of Action, and Biosynthesis of a Novel Lipodepsipeptide Antibiotic, WAP-8294A2
… We successfully identified the WAP-8294A NRPS biosynthetic gene cluster in L. staphylocidin. It is composed of two large open reading frames (ORFs) that encode NRPS machinery made up of 44 domains organized into 12 modules. The organization of the gene cluster argues for a co-linear …
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Characterizing and Engineering Thioesterases as Biocatalysts for Macrocyclization
… of two non-ribosomal peptide synthetase (NRPS) TE domains and their use as biocatalysts for the chemoenzymatic synthesis of two cyclic depsipeptide NP. Unlike other characterized NRPS TEs, these two demonstrated the ability to catalyze macrolactonization with minimal competing hydrolysis …
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A genomics-led approach to deciphering heterocyclic natural product biosynthesis
… synthase-nonribosomal peptide synthetase (PKS-NRPS) pathway in Streptomyces sp. KO-7888 and Streptomyces sp. OM-5714. The phthoxazolin pathway was used as a model to study 5-oxazole and primary amide formation in PKS-NRPS pathways. An unusually large gene cluster for phthoxazolin biosynthesis …
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Investigation of the antibacterial activity and the biosynthesis gene cluster of the peptide antibiotic feglymycin
… um ein nicht-ribosomal synthetisiertes Peptid (NRPS) handelt dessen Biosynthese der Biosynthese der Glycopeptidantibiotika der Vancomycin-Gruppe von Antibiotika ähnelt. Zudem konnten im Streptomyces sp. DSM 11171 Genom weitere NRPS und Polyketidsynthase (PKS) Gencluster identifiziert und …
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Identificación y caracterización de agrupaciones de genes de biosíntesis para péptidos no ribosomales en Streptomyces sp. CS113
… Sintetasas de Péptidos No Ribosomales (NRPS) o Policétidos Sintasas (PKS) respectivamente.
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Studies on the biosynthesis of oudenone and verucopeptin
… non-ribosomal peptide synthetase enzymes (NRPS). Based on the chemical structure of verucopeptin, the enzymatic motifs identified are consistent with those expected to be associated with its biosynthesis. A gene disruption experiment was performed with A. verrucosospora in order to …
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Catalytic C-H oxidation reactions for the synthesis and diversification of hydroxyamino acid motifs
… products of nonribosomal peptide synthetase (NRPS) origin posses complex topologies and diverse functional group arrays that lead to varied and impressive therapeutic potential. The structural diversity achieved among these natural products is due in large part to a biosynthetic strategy that …
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Stammdesign in B. licheniformis
… reduzierten Satz an potentiellen Prophagen. Drei NRPS-Gencluster mit Ähnlichkeiten zu Genen aus der Fengycin, Bacitracin und Lichenysin-Biosynthese konnten identifiziert werden.
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Discovering novel natural products from bioactive plants and bacteria
… from Streptomyces griseus, containing a PKS/NRPS hybrid gene, was chosen as a model system. To decipher this cluster, I have been attempting to develop a new strategy based on our recently developed “DNA assembler” method. By using this approach, the gene cluster was modified by adding …
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Magneto-optical oxide thin films and integrated nonreciprocal photonic devices
… demonstrated. Strong nonreciprocal phase shift (NRPS) has been demonstrated in chalcogenide glass/magnetic oxide and magnetic oxide/silicon strip-loaded waveguides by numerical simulations. A nonreciprocal optical racetrack resonator based on polycrystalline garnet/silicon strip-loaded waveguides …
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A functional genomics approach: Characterisation of two Non-ribosomal peptide synthetase genes, and investigation of an adaptive response towards alkylating DNA damage in Aspergillus fumigatus.
… this work further highlights the importance that NRPS plays in this serious human pathogen, and uncovered some interesting features such as possible secondary metabolite cluster cross-talk and NRP synthetase redundancy, themes which are beginning to emerge in the literature.
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