Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"NMR structure"”.
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"fine-tuning" of ribosomal structure and functions by pseudouridylation and rna-protein interactions
<p>ABSTRACT</p> <p>"Fine-tuning" of ribosomal structure and functions by pseudouridylation and RNA-protein interactions</p> <p>by</p> <p>JUN JIANG</p> <p>AUGUST 2012</p> <p>Advisor: Prof. John SantaLucia Jr.</p> <p>Major: Chemistry (Biochemistry)</p> <p>Degree: Doctor of Philosophy</p> <p>Ribosomal …
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Structural and biochemical insight into the interactions of Cdc42 with TOCA1 and N-WASP
… to achieve stable binding in vivo. The solution NMR structure of the Cdc42 binding HR1 domain of TOCA1 provides the first structural data on this protein and reveals some interesting structural features that may relate to binding affinity and specificity. A structural model of the Cdc42-HR1 …
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Novel Algorithms for Automated NMR Assignment and Protein Structure Determination
<p>High-throughput structure determination based on solution nuclear magnetic resonance (NMR) spectroscopy plays an important role in structural genomics. Unfortunately, current NMR structure determination is still limited by the lengthy time required to process and analyze the experimental data. A …
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Nucleosome-independent permanently condensed chromosome: investigation of novel nuclear organisation in the dinoflagellates
… strand en masse. I then endeavoured to solve the NMR structure of DVNP. The results suggest that DVNP plays a central role in chromatin packaging in dinoflagellates. Finally, to marry the results obtained from both experiments, at the end of the thesis I propose a model of a novel …
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Structural basis of membrane targeting and regulation of the innate immunity adaptor TIRAP by its phosphoinositide-binding motif
… (DPC) micelles and other membrane mimics. NMR studies reveal that TIRAP PBM binds PIPs following a fast exchange regime with a moderate affinity through two conserved basic termini. Mutation of these two basic regions abolishes PIPs binding without distorting the helical structure of the …
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Structural And Mechanistic Elucidation Of The EF-hand In LETM1 Ca2+/H+ Antiporter Function
… on secondary, tertiary and quaternary structure, thermal stability and high-resolution structure. We discovered the LETM1 EF1 features weak (~mM) but specific Ca2+ binding affinity that is responsive to both changes in pH and temperature within the physiological range experienced by …
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Structural analysis of LMO2 for the development of a small molecule inhibitor
… project were to structurally determine LMO2 for structure based drug development of small molecules that will target LMO2 protein-protein interactions. LMO2 could not be purified alone as removal of the fusion tag resulted in severe precipitation of the free LMO2. Consequently, LMO2 was …
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Nmr Solution Structures Of Two Hairpins Of E. Coli 16s Rrna: The Effects Of Mutations And Chemical Modifications On Structure And Function Of Rrna
<p>The structures of two functional mutants, the UC (G690U, U697C) and the QM mutants (G690A, G693C, A695C, U697A) of the 690 hairpin of E. coli 16S ribosomal RNA were determined by NMR. The UC mutant and the QM mutant with high biological function are able to fold into structures that are …
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Exploring the Biophysical Properties of Domains Within Multi-Domain Proteins
… of 7AW does not significantly perturb the structure or function of the isolated domain. Ligation of 7AW labeled SH3 domain to the c-Crk-I Src homology 2 (SH2) domain, via EPL, generated the multi-domain protein, c-Crk-I, with a domain specific label. Studies on this labeled protein show …
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Biomolecular NMR studies of the structure and biosynthesis of RiPP natural products
… link between genetic sequence and chemical structure. Because of this, bioinformatic analysis allows relatively easy prediction of final chemical structure, and opens the possibility for analog generation through relatively simple genetic modifications. Secondly, recent advances in genome …
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Part I: Effect of Nanomicelle Size on Trans-scleral Permeability of Dexamethasone and Part II: Strategies to Minimize Octreotide Acylation during Sustained Release from Biodegradable Polymers
… Polymers were characterized by H¹ NMR (structure), gel permeation chromatography (molecular weights and polydispersity), critical micelle concentration (CMC) and in vitro cytotoxicity studies in corneal, conjunctival and retinal cell-lines. Newly synthesized polymers were purified …
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Studies on the mechanism of action of the antimicrobial S-linked glycopeptide sublancin
… stability of sublancin, the three-dimensional NMR structure was solved, as described in chapter 2, revealing that hydrophobic interactions as well as hydrogen bonding are responsible for the stable and well-structured peptide. Unlike better-understood natural products, the molecular target of …
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The isolation, structure, and membrane interactions of biologically active peptides.
… presented in this thesis details the isolation, structure determination and mechanistic pathways of a selection of biologically active peptides. The southern brown tree frog Litoria ewingi occupies areas of the southeastern coast of Australia and Tasmania. Over a twelve month period, the peptide …
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Structural studies of the deaminase domain of the human HIV-1 restriction protein APOBEC3G
… of APOBEC3G (A3G-ctd) derivative, determine the NMR solution structure of this variant (A3G-2K3A) and characterize its interaction with single-strand DNA. Due to intrinsically low solubility of A3G-ctd, a strategy to design more soluble derivatives of the catalytic domain was performed prior to …
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Nuclear magnetic resonance study of the structure of simple molecules adsorbed on metal surfaces: Acetylene on platinum
We have used NMR to determine the structure of acetylene (HC~CH) adsorbed at room temperature on small platinum particles by studying the l3c_13c, l3C_1H, and lH_1H dipolar interactions among the nuclei in the adsorbed molecules. We find a model of 77% CCH2 and 23% HCCH to be the only one …
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Estudio de las modificaciones conformacionales y su influencia en la actividad inmunológica de péptidos derivados de las familias de las proteínas spect-1 y -2, siap-1 y -2 de plasmodium falciparum
SPECT-1 y -2 y SIAP-1 y -2 son proteínas pertenecientes al esporozoíto de Plasmodium falciparum causante de la malaria más agresiva en los humanos. Estas proteínas están involucradas en el paso del parásito a través de las células del hospedero humano y en la invasión del hepatocito, haciéndolas …
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The Structural and Functional Study of GIT1 Paxillin Binding Domain
… this dissertation, we determined the solution structure of rat GIT1 PBD by nuclear magnetic resonance (NMR) spectroscopy. The PBD folds into a four-helix bundle, which is structurally similar to the focal adhesion targeting (FAT) domain and the vinculin tail (Vt) domain. The PBD is more stable …