Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"NMD"”.
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Physiological role and quantitative aspects of human Nonsense-mediated mRNA decay (NMD)
Nonsense-mediated mRNA decay (NMD) is a molecular pathway of mRNA surveillance that ensures the rapid degradation of mRNAs containing premature translation termination codons in all studied eukaryotes. Originally, NMD was thought of as a quality control pathway that targets non-functional mRNAs …
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Characterization of nonsense mediated mRNA decay in Schizosaccharomyces pombe
Nonsense mediated mRNA decay (NMD) is a translation-coupled process that preferentially destroys mRNAs harboring premature translation termination codons (PTCs). In mammalian cells, NMD is linked to pre-mRNA splicing. Typically, PTCs elicit strong NMD only if positioned upstream of at least one …
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Harnessing transcriptomic data to better understand gene regulatory mechanisms of neurodevelopmental disorders
… investigates the nonsense-mediated mRNA decay (NMD) pathway —a key post-transcriptional mechanism with critical implications for neurodevelopment and NDDs —using transcriptomic data.. To assess the role of NMD, I first create a novel framework which utilises a data-driven approach to identify in …
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An investigation of gene expression, alternative splicing and nonsense-mediated decay in potato in response to Phytophthora infestans infection
… the activation of the nonsense-mediated decay (NMD) pathway; a conserved RNA surveillance mechanism. NMD targets aberrant endogenous transcripts for degradation. These transcripts contain features that allow them to be recognised by the NMD machinery: for example, premature termination codons …
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Régulation de voies de signalisation par deux acteurs de la voie Nonsense-mediated Decay, Dhx34 et Nbas: la cas particulier de l'adénohypophyse.
… mRNAs. Amongst them the Nonsense-Mediated Decay (NMD) pathway is the surveillance mechanism that detects and degrades mRNAs containing premature termination codons (PTCs). The translation of transcripts containing such nonsense codon within the coding sequence would leads to the production of …
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Isolation and characterization of Hupf1-containing protein complexes and their role in nonsense-mediated mRNA decay
… degraded by the nonsense-mediated mRNA decay (NMD) pathway. The mechanism of NMD in mammals is largely unknown. The cytoplasmic protein Hupf1 was the only trans-acting factor of NMD in mammals that was known at the beginning of this study. In order to identify and characterize proteins that are …
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Understanding regulation of mRNA by RNA binding proteins
… are regulated by nonsense-mediated mRNA decay (NMD), a quality control degradation pathway. In this thesis, I present a novel method for high throughput characterization of the binding affinities of proteins for mRNA sequences and an integrative analysis of NMD using deep sequencing data. This …
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Messenger RNA Destabilization by -1 Programmed Ribosomal Frameshifting
… destabilization through the nonsense mediated (NMD) and no-go (NGD) decay pathways. Yeast EST2 mRNA is highly unstable and contains up to five -1 PRF signals. Ablation of the -1 PRF signals or of NMD stabilizes this mRNA. These same computational methods identified an operational programmed -1 …
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Alternative Mrna Splicing Redefines The Landscape Of Commonly Dysregulated Genes Across The Acute Myeloid Leukemia Patient Population.
… the nonsense-mediated mRNA degradation (NMD) pathway motivated a query of splicing variations in an additional set of related NMD factors. Although no particular study has highlighted genetic mutations in the queried NMD factors, I find significant variation at the level of mRNA splicing …
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Expanding the genetic code of a mouse
… by deacetylase. Third, mRNA was effected by NMD(nonsense-mediated mRNA decay) that can break mRNA containing amber condons. I find the reason that low GFP expression was due to the degradation of mRNA through NMD To investigate GFP signal in AcKRS.GFP mouse I did cryosection and observed by …
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The identification and characterization of the interaction between Upf1 and PAB1 during nonsense-mediated decay & the identification of novel protein complexes associated with translation termination factor eRF1
… under investigation is nonsense-mediated decay (NMD), which rapidly degrades mRNAs that contain a premature stop codon (PTC). I identified that the interaction between Upfl, which is required for NMD, and PAB1 occurs via the RRM1 domain of PAB1 in the yeast Saccharomyces cerevisiae. Determining …
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Second-step splicing factors and heavy metal stress management in Arabidopsis thaliana
… translation-dependent nonsense-mediated decay (NMD) was involved in the accumulation of pre-mRNAs, the effects of chemically inactivating translation were examined. My studies have shown that the patterns of second-step splicing factor transcript accumulation observed when NMD is chemically …
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Nonsense-mediated mRNA reduction and pre-mRNA processing in drosophila
… system or nonsense mediated mRNA decay (NMD). This phenomenon has been extensively studied in budding yeast and in mammalian systems and to a lesser extent in <i>C. elegans</i>. In yeast the recognition of the nonsense codon appears to occur during cytoplasmic translation and premature …
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Regulation of the hif-1-dependent hypoxic stress response by C. elegans
… loss of the conserved nonsense-mediated decay (NMD) pathway, an RNA surveillance mechanism that degrades aberrant mRNA transcripts with premature termination codons, suppressed the egl-9(lf)-induced changes in egg laying and defecation and caused increased hypoxia sensitivity. Other aspects of …
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Multiple regulatory layers in the establishment of Rbfox2 splicing networks
… are coupled to nonsense-mediated mRNA decay (NMD). Regulation of NMD-coupled splicing by Rbfox2 alters gene expression of autoregulated RBPs and hundreds of additional genes. These observations place Rbfox2 upstream of a large network of direct and indirect splicing changes and offer an …
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OPTIMIZATION OF AAV9 GENE THERAPY FOR SPINAL MUSCULAR ATROPHY WITH RESPIRATORY DISTRESS TYPE 1 USING IN VIVO DISEASE MODELS
… intracerebroventricularly in SMARD1 mice model (nmd), during the presymptomatic phase of the disease at postnatal day 1. This comparison allowed us to determine which is the best construct to be translated to the clinic. Then, with the aim to precisely define the therapeutic window, we evaluated …
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Molecular and functional characterisation of circadian clock regulated RNA-binding proteins from Arabidopsis thaliana
… are impaired in the nonsense-mediated decay (NMD) of RNAs. Indeed, the alternative AtGRP splice forms are increased in the upf mutant plants, indicating a previously unknown connection of the circadian clock and the NMD pathway.
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Impacts of legacy mining on stream structural and functional processes in an urban stream
… specifically how neutral mine drainage (NMD) influences urban streams. Past research on both urban streams and mine impacted streams has largely focused on characterizing streams using structural approaches although there has been an increase in literature on functional processes over the …
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Tracking translation factors in fission yeast nucleus
… in S. cerevisiae. Nonsense-mediated mRNA decay (NMD) removes any mRNA containing premature termination codon (PTC), and requires Upf1. Phospho-Upf1 inhibits conversion of 40S/Met-tRNAiMet/mRNA to translationally competent 80S/Met-tRNAiMet/mRNA initiation complexes to repress continued translation …
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A clinical and molecular genetic study into familial and sporadic Parkinson’s Disease
… provide evidence that nonsense mediated decay (NMD) is occurring in LRRK2-PD. In this thesis I also present data from an extensive screen of LRRK2 in UK subjects with sporadic PD (sPD). This confirms the importance of pathogenic LRRK2 mutations in UK sPD. In addition I report a novel missense …
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