Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"NIH-3T3 cells"”.
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A role for glutathione in reactions between oxidants and proteins
… a cytosolic protein which is S-glutathiolated in cells under oxidative stress. The pure protein was found here to be S-glutathiolated by two oxidants, 2,2'-Azobis(2-amidinopropane)dihydrochloride (AAPH) and hydrogen peroxide (H2O2). At physiologically relevant molar ratios of GSH to protein, GSH …
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USING CHEMICAL AND PROTEOMICS APPROACHES TO STUDY THE FUNCTIONS OF SIRT6 AND KRAS
… colony formation in KRas4a transformed NIH 3T3 cells than in KRas4b transformed NIH 3T3 cells. Moreover, mTOR was identified as a KRas4a/b interaction protein. Different from well-established mTORC1 and mTORC2, KRas4a/b forms a new mTOR complex without raptor or rictor in cells.
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Role of an Adenylyl Cyclase Isoform in Alcohol's Effect on Cyclic AMP Regulated Gene Expression in Mammalian Cells
… hypothesize that the AC isoform expressed in the cells will play a role in ethanol’s effects on cAMP regulated gene expression. We further hypothesize that alcohol modulates cAMP signaling in immune cells by enhancing the activity of AC7; thus, AC7 may play a role in ethanol’s effects on immune …
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Characteristic analysis of mobility inducers for neural stem cells
Recently neural stem cells (NSCs) were reported to migrate to damaged brain areas, such as brain tumors or ischemic regions. Systemic strategy can be effective to cancer therapeutic effect through cell-based gene therapy. From our previous studies, we identified chemoattractant molecules-1(CM-1) …
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Polymorphisms in the Flt1 gene and their relation to expression of the secreted Flt1 variant
… Flt1 minigene plasmids were transfected into NIH/3T3 cells. Both exonic SNPs were associated with ~40% decreases in Flt1:sFlt1 mRNA ratios determined by real-time PCR. To facilitate exploration of ESEs in regulated RNA splicing, a PERL computer program, "EXONerator" was written to silence …
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Molecular and Functional Regulation of Akt2 by Oxidation
… regulation of Akt1 and Akt2 signaling in the NIH 3T3 cells. The role of NOX-derived and mitochondrial-based ROS in regulating Akt2 activity following PDGF stimulation is also elucidated. Finally, functional consequence of Cys124 oxidation on modulating Akt-regulated biological processes of …
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The Role of Sphingosine Kinase 2 in Cell Growth and Apoptosis
… it was found that when overexpressed in mouse NIH 3T3 fibroblasts SphK2 enhances caspase-dependent apoptosis in response to serum deprivation, independently of S1P receptors. Sequence analysis revealed that SphK2 contains a 9 amino acid motif similar to that present in BH3-only proteins. …
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A Role for RAS in V-CRK Transformation
… that might be activated in v-Crk transformed cells were investigated by analogy to pathways utilized by v-Src and the activated EGFR, and a search for serine/threonine kinases activated in v-Crk transformed CEF was conducted. Mapk was identified as one kinase, the activity of which is …
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ZIPRO1: Fine Tuning Proliferation in Discrete Cell Populations
… of expression in several tissues. The main cells expressing Zipro1 are proliferating cells from epidermal lineages. This includes the cerebellar granule cells (GCs), nasal epithelia and dermal papilla. Through gene dosage experiments we have shown that Zipro1 is involved in proliferation …
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Regulation of Human T-cell Leukemia Virus Type 1 Infection and Replication
… screen, in which cDNA from permissive HeLa S3 cells was introduced into poorly susceptible NIH 3T3 cells. These cells were selected after infection with HTLV-1 envelope pseudotyped viral particles expressing a drug resistance gene. We isolated approximately 460 cDNAs, of which 20 were …
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Investigation of caveolae-mediated endocytosis in response to interferon
… Endocytosis, a vital process in healthy cells used to internalize material, is a likely IFN target. The anti-tumor nature of IFN may be an unintentional byproduct of its antiviral effects, which are designed to halt pathogenic assault, including pathogen entry into the cell via [2,3]. …
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Single-shot quantitative interferometric microscopy for imaging high-speed dynamics
… prior knowledge from a training dataset of ⇠ 900 NIH/3T3 cells, we realized single-shot 3D cell imaging at a 3D imaging speed of over 10,000 fps, enhancing the throughput of 3D flow cytometer to over 5000 cells per second. These technology advances open the horizons in which the single-shot …
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Myxoid Liposarcoma: Models and Mechanisms of Sarcomagenesis and Response to Radiation Therapy
… but not Kras-driven murine sarcoma cells. ChIP-seq and CUT&RUN profiling of human MLPS cell lines identified DNA-binding motifs and genomic loci targeted by FUS-CHOP, which co-localized with SNF2H and H3K27ac marks of active chromatin. We further hypothesized that post-translational …
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Using imaging secondary ion mass spectrometry to determine mammalian plasma membrane component distribution and cell differentiation state
… of the plasma membranes of mammalian cells has been studied for over forty years, with our understanding evolving from the fluid mosaic model of Singer and Nicholson to a more nuanced model acknowledging lateral heterogeneity in protein and lipid distributions. Within this area of …
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Characterisation of open reading frames m29 and m29.1 in murine cytomegalovirus
… BACs by in vitro passage in tissue culture cells. Revertant virus (Rv29.1) was made by a further 2 step process in which the mutant m29.1 ORF was first replaced by the antibiotic cassette and then by the wt ORF. These mutants were characterized both in tissue culture and in immunocompetent …
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The Impact of substrate texture on cell behavior
… effects of physical cues on the behaviour of the cells. Development of fabrication techniques has allowed researchers to design topographical cues at the micro and nanometer scale. In this study, we fabricated fibronectin stripes, which mimic some of the frequent patterns that cells are exposed to …