Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"NCp7"”.
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The Study of Au(III) Compounds and their Interaction with Zinc Finger Proteins
… histidine) HIV nucleocapsid zinc finger protein, NCp7. NCp7 is involved in multiple steps of the HIV life cycle, thus making it a desirable drug target. Previous studies from our group show platinated nucleobases such as [Pt(dien)(9-EtG)]2+ (dien = diethylenetriamine; 9-EtG = 9-ethylguanine) to …
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Mutational Analysis of HIV-1 Nucleocapsid Protein and Methods Development for Aptamer Discovery
… analysis of HIV-1 nucleocapsid protein (NCp7). Sixteen mutants of NCp7 were constructed and over-expressed in <em> E. coli </em> . The dissociation constants (K <sub>d </sub> ) between the mutant proteins and the major packaging signal in HIV-1, SL3 RNA, were measured using tryptophan …
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Platinum Complexes and Zinc Finger Proteins: From Target Recognition to Fixation
… experiments used transplatin to crosslink NCp7 to viral RNA in order to understand how/where the protein bound to RNA. We have studied the interaction between cis and trans dinuclear platinum complexes and the C-terminal zinc finger (ZF). The trans complex reacts at a faster rate than the …
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Targeting RNA With Small Molecules: SL3 of HIV -1 Psi-Rna
… its interaction with viral nucleocapsid protein, NCp7. Since SL3 is not a target of current anti-HIV therapeutics, the discovery of small molecules that target SL3 would not only expand the understanding of RNA recognition by small molecules, but could also lead to discovery of novel anti-viral …
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NONCOVALENT INTERACTION OF PLATINUM PLANAR AMINE COMPOUNDS WITH TRYPTOPHAN: A STRATEGY TO INTERFERE WITH P53-MDM2 INTERACTIONS AND TARGETING RETROVIRAL ZN FINGER-DNA INTERACTION (HIV NCP7)
<p>Non-covalent interactions involving π-π stacking play an essential role in self-assembly and molecular recognition processes such as protein folding and DNA/RNA-protein selective recognition. The knowledge gained from these studies could provide insight into possible site recognition complexes, …
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Molecular Sensing with Protein and Solid-State Nanopores
… binding of the nucleocapsid protein of HIV-1 (NCp7) to three aptamers of different affinity, specifically three sequence 20mer mimics of the stem-loop 3 (SL3) RNA--the packaging domain of genomic RNA. Additionally, N-ethylmaleimide, which is known to inhibit the binding of NCp7 to a …
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Study Of Covalent And Non-Covalent Interactions In Ternary Systems Involving: Metal/DNA-RNA/Protein, Where Metal = Platinum(II), Palladium(II)
… in HIV-1 virus, i.e. nucleocapsid protein NCp7. Findings showed that the nucleobase ligand in addition to modulate hydrolysis and reaction rates for MPNs can also be responsible for an initial non-covalent recognition towards a specific protein. This initial recognition has been proposed as …