Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"NBS1"”.
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THE FUNCTION OF MRN (MRE11-RAD50-NBS1) COMPLEX DURING WRN (WERNER) FACILITATED ATM (ATAXIA-TELANGIECTASIA MUTATED) ACTIVATION
… fork collapse. Because the Mre11-Rad50-Nbs1 (MRN) complex, a sensor of DSBs, is known to interact with WRN and ATM, we investigated whether the MRN complex mediates the WRN-dependent ATM pathway activation. In this study, we employed short-hairpin RNA to generate WRN- and Nbs1-deficient …
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The Role of E2F1 In The Response to Dna Double Strand Breaks
… In response to DNA double-strand breaks, NBS1 phosphorylation and foci formation are defective in cells lacking E2F1, but NBS1 expression levels are unaffected. Moreover, it was observed that an association between NBS1 and E2F1 is increased in response to DNA damage, suggesting that E2F1 …
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The Rad51 family of proteins: Interactions, vitamin D, and implications in head and neck cancer
… VD 3 is concomitant with the down-regulation of NBS1. NBS1 is a DNA repair protein involved in both pathways of DNA double-strand break repair, non-homologous end-joining and homologous recombinational repair. It has recently been demonstrated that NBS1 binds to Rad51 aiding in its localization …
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Studio del meccanismo di riparazione del DNA nelle leucemie in età pediatrica: alterazioni dei geni NBS1, Fancd2, Palb2 ed espressione dei geni BRCA1 e BRCA2.
… il gene della sindrome da rottura di Nijmegen (NBS1). Abbiamo voluto la correlazione tra alterazioni del meccanismo di riparazione del DNA e l’insorgenza di recidive e/o con tossicità grave (grado III-IV – CTCAE) durante il trattamento chemioterapico.
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The interaction of CtIP with DNA damage response proteins
… containing BRCT domains: 53BP1, MDC1, TopBP1 and NBS1. The binding sites on all of these proteins have been mapped establishing which regions of CtlP interact directly with 53BP1, MDC1, TopBP1 and NBS1 and vice versa. This implies that CtlP is involved in the DNA damage response on many levels …
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Caretaker-Gen-Syndrome
… Prozent aller NBS-Patienten tragen Mutationen im NBS1-Gen, dessen Translationsprodukt im Komplex mit MRE11 und RAD50 eine zentrale Rolle in DNA-DSB-Reparatur und Zellzykluskontrolle spielt. Weitere Mutationen bei Patienten mit NBS-ähnlichem Phänotyp wurden im Gen der DNA-Ligase IV identifiziert, …
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Distinct DNA Damage Signaling in the Brain Distinguishes ATLD, NBS, and ATR-Seckel Syndrome
… system. Hypomorphic mutations in the Mre11/Rad50/NBS1 complex (MRN complex), which is required for efficient ATM activity, result in distinct syndromes. Ataxia-telangiectasia-like disease (ATLD) results from truncating mutations in Mre11 and presents with neurodegeneration similar to that observed …
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Widespread Phosphorylation of H2AX by Adenovirus Requires Viral Genome Replication and the E1B-55k or E4orf3 Proteins
… of the sensor complex composed of Mre11/Rad50/NBS1 for degradation and relocalization, as well as targeting the effector protein DNA ligase IV. Despite inactivation of these cellular sensor and effector proteins, infection results in high levels of histone 2AX phosphorylation, or γH2AX. …
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How is Ataxia-Telangiectasia Mutated Protein Kinase Activated?
… DNA. This damage is detected by the Mre11-Rad50-Nbs1 (MRN) complex, which recruits ATM to the DNA. Upon activation, ATM phosphorylates a vast range of substrates, which triggers a multitude of downstream pathways such as DNA damage repair, cell cycle arrest, senescence and sometimes apoptosis. In …
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UNRAVELING THE MOLECULAR MECHANISM OF BRCA2-POLTETA INTERPLAY IN PREVENTING SSDNA GAP ACCUMULATION DURING DNA REPLICATION
… source of DNA damage and we find that MRE11-NBS1-CtIP nuclease is responsible for this process. We further investigate the origin of ssDNA gaps and find that they mainly arise from SMUG1 processing of demethylation intermediates, such as 5hmC, leading to the generation of abasic (AP) sites. …
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Single-molecule studies reveal mechanisms of human DNA double-strand break repair
… Ku70-Ku80 heterodimer (Ku) and the Mre11-Rad50-Nbs1 complex (MRN), respectively. Ku encircles the DNA ends and recruits other factors, such the kinase DNA-PKcs, to bluntly ligate the ends back together. In contrast, MRN along with the long-range nuclease Exo1 and helicase BLM digests the DNA to …
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Mechanism of Mismatch Repair Induced Mutagenesis in Somatic Hypermutation
… addition, I explore the role of the Mre11-Rad50-Nbs1 complex in its contribution to A:T mutations in Ramos cells. Taken together, these studies demonstrate that conversion of classical DNA repair pathways into mutation-generating processes is driven by the unique environment of the V region in …
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Brit1/Mcph1 Mediates The Dna Damage Response By Inducing P53 Stability and Promoting Atr Signaling
… repair proteins including 53BP1, MDC1, NBS1, and the SWI/SNF complex to the DSB region to promote DNA repair. <em>BRIT1</em> copy number deficiency correlates with increased genomic instability in ovarian cancer specimens and breast cancer cell lines. Here, we propose that additional …
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Mechanisms That Ensure the Proper Terminal Structure of Mammalian Telomeres
… end-processing step does not appear to require Nbs1 or BLM, and its physiologic role remains unknown. This thesis supports a model in which leading- and lagging-end telomeres have different requirements for end-processing by the nucleases Apollo and Exo1. The binding of POT1b on appropriately …
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Novel functions of N-acetyltransferase 10 (NAT10) in DNA repair and replication with potential implications for premature ageing syndromes
… the complex of three proteins: MRE11, RAD50 and NBS1/NBN (MRN complex). This complex plays an important role in the sensing and repair of DNA double-strand breaks (DSBs), highly genotoxic lesions that can result in unrestrained cellular lethality or genomic instability. During this project, I …
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Characterization of a Novel 53BP1-Dependent Mechanism that Promotes Non-Homologous End Joining of Deprotected Telomeres by Increasing Chromatin Mobility
… We have examined the roles of the Mre11/Rad50/NBS1 (MRN) complex, H2AX, MDC1, and 53BP1 in the NHEJ of dysfunctional telomeres. We have demonstrated that among these factors, 53BP1 is required for the fusion of telomeres, whereas the MRN complex, H2AX, and MDC1 only stimulate the efficiency of …
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Molecular Mechanisms of Mitotic Spindle Assembly and Accurate Chromosome Segregation
… cells. I show that the MRN (Mre11, Rad50, and Nbs1) complex is required for metaphase chromosome alignment. Consistent with the result of my colleague using Xenopus egg extracts, disruption of MRN function by depleting Mre11 using an inducible shRNA system, or Mre11 inhibitor mirin, triggers a …
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Estudio de la ubicuitilación del complejo MRN mediada por SCF(bTrCP/FBXW7): implicaciones biológicas
… doble cadena es el complejo MRN (MRE11, RAD50 y NBS1), esencial para la identificación de las roturas, el reclutamiento de diversos factores de señalización y el procesamiento, todo ello encaminado a la reparación de las mismas. El complejo MRN también interviene en otros procesos, como la …