Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"NATURAL SCIENCES::Biology::Cell and molecular biology"”.
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Investigating β- and γ-secretase function and processing of APP with genetic and pharmacological tools
… of Amyloid Precursor Protein (APP) by BACE1 and the gamma secretase complex (γS) generates amyloid-beta (Aβ), a small peptide that is highly implicated in the pathogenesis of Alzheimer’s Disease. Despite decades of research, there remains a lack of understanding of the endogenous functions of …
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Investigating the effects of aging and the neuromodulator retinoic acid on neurite outgrowth in a novel CNS organ culture
… impairs this process through changes in calcium handling, neurotransmitter sensitivity, and energy metabolism. In this thesis, I investigated agerelated changes in neurite outgrowth using a novel central nervous system (CNS) organ culture, in which outgrowth can be observed from cut nerves resting …
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Physiological Oxygen and Media Conditions Influence Breast Cancer Hallmarks in Vitro
Standard cell culture conditions often fail to replicate the in vivo microenvironment, limiting the reproducibility and physiological relevance of experimental findings. For example, commonly used media such as Dulbecco’s Modified Eagle Medium (DMEM) contain supraphysiological levels of key …
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Genome-wide microscopy screening identifies links across processes including a conserved connection between DNA damage control and the microtubule cytoskeleton
… high-content microscopy screening of a cell library consisting of over 3000 single mutant strains of the fission yeast, Schizosaccharomyces pombe. Each strain has one nonessential gene knocked-out, allowing investigation of the resulting phenotypes. I report the implementation and …
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Graph Attention Mechanisms for Modeling Pathway-Level Importance from Gene Expression
… technologies have revolutionized the analysis and profiling of gene expression data, enabling a comprehensive understanding of the complex mechanisms underlying molecular biology. However, the high dimensionality and noise inherent in gene expression data pose significant challenges for …
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Dissecting the anti-allergic potential of carnosic acid during IgE and IL-33-mediated mast cell activation
Mast cells are critical immune sentinels and essential regulators of inflammation, most notably recognized for their central role in allergic inflammation. Allergen-induced cross-linkage of FcεRI complexes initiates a biphasic mast cell response, characterized by the immediate degranulation of …
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Characterizing Zika and West Nile virus infection in mast cells and the antibody-dependent enhancement potential mediated by dengue virus antibodies
In the dermis, long-lived resident mast cells are well-situated to respond to mosquito-borne pathogens. Within the Flavivirus genus, dengue virus (DENV), Zika virus (ZIKV), and West Nile virus (WNV) are evolutionarily related mosquito-borne viruses with antigenic similarities. The antigenic …
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Determining the Roles of the Aldehyde Dehydrogenase Enzymes 5 and 6 during Icewine and Table Wine Fermentation in a Commercial Wine Yeast Using CRISPR-Cas9.
… known for Icewine which is fermented from grapes naturally frozen on the vine. Grapes are harvested below –8°C and pressed while frozen, trapping ice crystals inside the berries and releasing juice highly concentrated in sugars and acids. The high sugar juice environment (40°Brix) causes …
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The impact of acute heat exposure on the heat shock response (HSR) between males and females
… heat stress induces genomic changes in immune cells of young adults, the influence of ovarian hormones on the molecular response remains unclear, despite their documented role in thermoregulation. This study investigated the molecular response to acute heat stress in males (n=11), eumenorrheic …
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Regulation of Tubulin dynamics by the +Tip tracking protein Mal3
… network is a central component of the eukaryotic cell cytoskeleton. In the fission yeast S. pombe, a complex of three proteins specifically tracks MT +ends and stabilizes MTs in the cell. It is composed of the proteins Mal3, Tip1 and Tea2. Mal3, the S. pombe homologue of EB1, is a highly conserved …
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Computational methods for multi-omic models of cell metabolism and their importance for theoretical computer science
… science can help biologists, but also on how biology can inspire computer scientists. On one hand, computer science provides powerful abstraction tools for metabolic networks. Cell metabolism is the set of chemical reactions taking place in a cell, with the aim of maintaining the living state …
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Quantitative genetics of gene expression during fruit fly development
… well as quantitative phenotypes, by exploiting naturally occurring genetic variation in large cohorts of individuals. More recently, the GWAS approach has also been applied to highthroughput RNA sequencing (RNA-seq) data in order to find loci associated with different levels of gene expression, …