Global ETD Search
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Showing 1 to 15 of 15 for “"NAP1"”.
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THE INTERPLAY OF HISTONE CHAPERONE NAP1 WITH SWI/SNF FAMILY OF CHROMATIN REMODELLERS IN S.cerevisiae
… have investigated the role of histone chaperone Nap1 (Nucleosome Assembly Protein1) on the remodeling properties of the yeast remodeler RSC ((Remodels Structure of Chromatin). We focused on the effects of Nap1 when RSC remodels short nucleosomal arrays and compared these to those with …
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Mutational analysis of the human histone chaperone, Nap1, in nucleosome disassembly at the HTLV-1 promoter
… acceptor DNA and nucleosome assembly protein (Nap1). Tax and pCREB recruit the histone acetyltransferase, CBP/p300, which acetylates histone tails prior to disassembly of the octamer. Nap1 is unique in this reaction because this was the first example of a histone chaperone supporting …
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A prospective study of Clostridium difficile infection to investigate the impact of NAP1 strain in a tertiary referral hospital
Includes abstract. Includes bibliographical references.
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The structural analysis of histone H3 lysine 56 acetylation and related histone chaperone complexes
… a member of the Nucleosome Assembly Protein 1 (Nap1) chaperone family. Nap1 family chaperones adopt a characteristic homo-dimeric ‘headphone’ fold and are capable of binding H2A/H2B and H3/H4, the latter in its tetrameric conformation. Experiments described here reveal Vps75 and Nap1 form …
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The Safety and Efficacy of Oral Low-Volume Sodium Phosphate Bowel Preparation for Colonoscopy in Dogs
… of oral NaP in dogs. Standard (NaP and enemas; NaP1) and control preparations (polyethylene glycol [PEG] and enemas) were compared in a crossover design to determine safety and efficacy of NaP. Serial clinical and serum analytical evaluations were used to determine the safety of NaP. The …
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The Non-canonical Function and Regulation of TBK1 in the Cell Cycle
… response protein, NAK Associated Protein1 (NAP1/AZI2), is the adaptor required for binding and activating TBK1 during mitosis. Loss of either NAP1 or TBK1 results in the accumulation of binucleated and multinucleated cells, possibly due to several mitotic and cytokinetic defects seen in …
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BIOCHEMICAL CHARACTERIZATION OF SUPPRESSOR OF IKK-ε AND NAK-ASSOCIATED PROTEIN 1
… a scaffold protein, NAK-associated protein 1 (NAP1). The role of NAP1 in modulating kinase activation or regulation is unknown. A key inhibitory protein identified in the TLR3:interferon pathway, silencer of inhibitor of κBα kinase ε (SIKE), blocks the activity of this kinase complex through an …
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Characterisation of the (H3-H4)2-tetramer and its interaction with histone chaperones
… with two histone chaperones from S. cerevisaie, Nap1 and Vps75. Within the nucleosome H3 and H4 form a heterotetrameric structure sustained by the interface between two histone H3 proteins. Interestingly, when bound to the histone chaperone Asf1 the H3-H3’ interaction is disrupted, thus Asf1 …
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Responses to Antifungal and Alkaline pH Stress in Human Fungal Pathogens
… we identified nucleosome assembly protein 1 (Nap1) as an interactor of the Rra1 pH sensor. Like Rra1, C. neoformans Nap1 is required for the activation of the Rim pathway. Nap1 specifically interacts with the Rra1 protein, acting as a scaffold to maintain stability of the Rra1 pH sensor …
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Biochemical and structural studies of histone and associated proteins from chick and human nuclei
… histone dimers and tetramers, the complexes of NAP1-dimer and NAP1-tetramer were obtained in collaboration with others. Crystals of pure histone octamers with a higher resolution than before were produced (Chapter 2). A low KCI/phosphate wash of chick erythrocyte nuclei removed up to 1 g of …
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DYNAMICS OF NUCLEOSOME REMODELING BY ATP-DEPENDENT CHROMATIN REMODELERS
… coordinated action of RSC and histone chaperone Nap1. We also provide insights on how aberrant SWI/SNF complexes affect fundamental enzymatic properties such as ATPase and processive nucleosome remodeling. ISW2 remodelers sense and respond to the length of linker DNA separating the nucleosome and …
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Biochemical Reconstitution and Functional Characterization of the WAVE Regulatory Complex
… Complex (WRC). It consists of WAVE, Sra1, Nap1, Abi2 and HSPC300. Biochemical studies of WRC have been hampered by the difficulty of expressing WRC components in bacterial, insect or yeast expression systems. Baculoviruses yielding high expression of each component of WRC were obtained …
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Antigenic Variation and Telomere Structure in Trypanosoma brucei
… by the trypanosome nucleosome assembly protein NAP1.</p>
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Characterization of intracellular interactions between dengue virus and host proteins
… protein interactions, I selected to study capsid-NAP1 interaction. I employed the domain mapping of capsid using yeast two-hybrid and co-affinity purification. I also over-expressed or silenced NAP1L1 in HepG2 cells stably expressing capsid. I found that NAP1L1 might bind the bipartite sequence of …
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Making Molecular Movies: Using Single-Molecule Techniques to Unveil Hidden Features of Protein-Chromatin Interactions
… a new method that utilizes the histone chaperone Nap1 to directly assemble nucleosomes on DNA substrates stably tethered within a smCFFM instrument. In addition to more closely resembling the physiological pathway of nucleosome formation in vivo, this method allows users to reconstitute …