Global ETD Search
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Showing 1 to 20 of 20 for “"N6-methyladenosine"”.
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Structures and Regulation of the N6-Methyladenosine Methyltransferase METTL16
… The most abundant mRNA modification is N6 methyladenosine (m6A), which has been shown to regulate RNA splicing, translation and decay. The m6A modification is deposited by methyltransferases using S-adenosylmethionine (SAM) as the methyl donor. Determining the specificity and mechanism …
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INTERROGATING THE ROLE OF N6-METHYLADENOSINE RNA MODIFICATION ON TELOMERASE BIOLOGY
… mature hTR. My work has identified hTR to be an N6-methyladenosine (m6A) modified RNA. The presence of m6A adds another layer of complexity to hTR processing due to the ability of the mark to be added to specific RNA and act as a target for m6A selective RNA binding proteins. In the case of hTR, …
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Role of N6-Methyladenosine (m6A) epitranscriptomic mechanisms in manganese-induced astrocyte inflammation
… at the time of this writing, namely that of N6-methyladenosine (m6A) epitranscriptomics and its regulatory tiers that control the writing, erasing, and reading of m6A modified RNAs. We find that the global m6A modifications are significantly affected in Mn exposed astrocytes. More …
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THE EPITRANSCRIPTOMIC REGULATION OF STRESS: ROLE OF N6-METHYLADENOSINE IN ABIOTIC STRESS RESPONSE
… More specifically, studies have shown that N6-methyladenosine(m6A), the most prevalent internal mRNA modification, can alter gene expression by affecting the stability, processing, and translation of mRNA among other functions. Very few studies have explored the involvement of m6A in the …
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Genetic screens of RNA binding proteins identify a role for N6-methyladenosine in promoting B cell differentiation
… investigate the role of the RNA modification N6-methyladenosine (m6A) in B cells. This work demonstrated a role for the m6A binding protein Ythdf2 in promoting B cell terminal differentiation, independently of a role in proliferation or survival. In the future, I hope to validate an in vivo …
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Elucidating The Direct Role of M6A In The Human Transcriptome
… methylation of adenosine at position 6 to give N6-methyladenosine (m6A). m6A modification on cellular RNAs was discovered in the 1970s. The past decade has witnessed major progress that illustrated important roles of this mark in various aspects of gene control. Due to its pleiotropic roles, the …
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ARCHITECTURE AND RNA RECOGNITION BY THE EUKARYOTIC M6A METHYLTRANSFERASE COMPLEX
… diverse biological functions. Among them, N6-methyladenosine (m6A) is one of the most abundant mRNA modifications and contributes to key cellular processes, including mRNA processing, translation, and decay. In eukaryotes, m6A is installed by the m6A Methyltransferase Complex (MTC), a …
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Role of m<sup>6</sup>A RNA "reader" YTHDF2 in Mammalian Germ Cells and Early Embryo Development
… specifically in cell fate determination. N6-methyladenosine (m6A) is one of the most abundant and conserved internal mRNA modifications that plays important role in regulation of gene expression in development and disease. The m6A-RNA modification mostly destabilizes mRNA levels and its …
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Uncovering the Roles of Human C2H2-Zinc Finger Protein ZNF121 in Gene Regulation
… RNA are one type of gene regulatory mechanism. N6-methyladenosine (m6A) is the most abundant and well-characterized mRNA modification, facilitating mRNA regulatory processes that include mRNA decay regulated by the m6A reader proteins YTH-domain-containing family protein 2 (YTHDF2). However, …
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CRISPR AS A TOOL FOR TARGET DISCOVERY AND TECHNOLOGICAL INNOVATION: HIGH-THROUGHPUT CBE SCREENING TO EVALUATE M6A MODIFICATION PATHWAY IN CANCER PROLIFERATION AND DRUG RESISTANCE, AND INNOVATIVE PRIME EDITING STRATEGIES FOR DISEASE MODELLING AND THERAPY
N6-methyladenosine (m6A) is the most abundant internal RNA modification of mRNA in mammals. The m6A -pathway comprises numerous effectors, which are classified based on their function. m6A is deposited co-transcriptionally by “writers”, including methyltransferase-like 3 (METTL3) and …
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Activin/Nodal Signalling Controls the Epigenome and Epitranscriptome of Human Pluripotent Stem Cells
… complex to promote the formation of N6-methyladenosine (m6A). This epitranscriptional modification antagonizes the expression of selected mRNAs, including pluripotency factors whose transcription is promoted by SMAD2/3. Therefore, this provides a negative feedback that facilitates …
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Epitranscriptional Control of the Caspase-2—MDM2—p53 Axis: The Role of the m6A Writer in p53 Signaling.
… This study identifies an unexpected role for N6-methyladenosine (m6A) RNA methylation as an essential post-transcriptional determinant of p53 activation in response to centrosome-derived stress. Using a genome-wide CRISPR/Cas9 screen coupled with a fluorescent reporter system, we uncovered all …
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An Inverse Electron-Demand Diels-Alder Strategy for Mapping RNA Methylation
Post-transcriptional RNA modifications, such as N6-methyladenosine (m6A), 2'-O-methylation (Nm), and 5-methylcytosine (m5C), are critical regulators of gene expression. Mapping these modifications at single-nucleotide resolution, however, remains a major challenge as current methods often suffer …
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Computational Analysis of Transcriptional Regulation
… an RNA post-transcriptional modification (RPTM), N6- methyladenosine (m6A), in buffering transcriptional noise. Maternally-supplied YTHDF2 is essential for degradation of m6A-modified transcripts during the maternal-to-zygotic transition (MZT) early in mammalian embryogenesis. YTHDF2 targets …
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Investigation of m6A-regulated RNA-binding proteins
N6-methyladenosine (m6A) is one of the most ubiquitous modifications of eukaryotic RNAs. The modifi- cation functions to control the normal processing, export, splicing, and metabolism of cellular RNAs. All of these processes are mediated by a group of m6A RNA-binding proteins known as ‘readers’ …
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M6A Reshapes the Folding and Recognition Landscape of RNAs
… have profound effects on RNA metabolism. N6-Methyladenosine (m6A) is an abundant post-transcriptional RNA modification that influences multiple aspects of gene expression. While m6A is thought to mainly function by recruiting reader proteins to specific RNA sites, the modification can also …
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Novel treatment strategy for ALK-positive Anaplastic Large Cell Lymphoma
… the expression of an RNA methyltransferase, N6-adenosine-methyltransferase 70 kDa subunit (METTL3). This enzyme catalyses N6-methyladenosine (m6A), the most abundant mRNA modification, through a complex with METTL14 and WTAP. Whilst METTL3 has been described as both an oncogene and a tumour …
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Characterizing novel molecular regulators of antiviral gene expression
… regulatory controls is the RNA base modification N6-methyladenosine (m6A), which regulates many aspects of mRNA metabolism through transcript-specific effects. m6A deposition is mediated by a cellular complex of proteins including METTL3 and METTL14 (METTL3/14) and other cofactors, and m6A can …
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Posttranscriptional regulation with regard to cleavage factor I (CFI) associated alternative polyadenylation and methyltransferase A (MTA) assisted m6A modifications in Arabidopsis thaliana
… well-studied in humans, but not in Arabidopsis. N6-methyladenosine (m6A) is one of the most frequently observed RNA modifications, which is established by methyltransferase A (MTA) and other m6A writer proteins in Arabidopsis thaliana.<br /> This study revealed RNA binding sites of CFI subunit …