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Showing 1 to 20 of 58 for “"N. benthamiana"”.
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The Development of an African Horse Sickness Virus VP7 Quasi-Crystal Vaccine Candidate in N. benthamiana
… 5 (AHSV-5) VP7 was expressed in Nicotiana benthamiana by means of Agrobacterium-mediated infiltration of plant expression vector pRIC3.0 encoding VP7 and quasi-crystals purified by means of density gradient ultracentrifugation. The presence of AHSV VP7 quasi-crystals was confirmed by …
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Characterization of Effector Genes in Acidovorax citrulli the Causing Agent of Bacteria Fruit Blotch Disease of Cucurbits
… constructed. The model plant species Nicotiana benthamiana was found to be susceptible to A. citrulli, while Nicotiana tabacum was resistance to A. citrulli, so therefore could carry nonhost resistance genes. Two T3S effectors, Aave1548 and Aave2166, triggered water soaking-like cell death in N. …
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Engineering the Nicotiana benthamiana secretory pathway for the production of Lujo virus vaccines and diagnostic tools
… a production pipeline in HEK293T cells and N. benthamiana for the protein LUJV GP-C∆TM (Lujo virus glycoprotein precursor without the transmembrane region). The LUJV GP-C∆TM was constructed from gene sequences of the envelope glycoprotein of Lujo virus and then adapted to production in HEK293T …
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Development of microalgae as a biopharming platform
… by comparing to replication in Nicotiana benthamiana by quantitative polymerase chain reaction (qPCR). Western blot results indicated EGFP expression in N. benthamiana but not in Scenedesmus. By using PCR the presence of EGFP DNA in Scenedesmus was detected but qPCR showed no increase of …
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Maize R gene Rxo1 Confers Disease Resistance on Pepper and Nicotiana benthamiana
… into the far-related dicot species Nicotiana benthamiana and pepper, and characterized the Rxo1-mediated disease resistance against Xe strains carrying AvrRxo1 or Xe4428. In addition, we explored the virulence function and mechanism of Xe4428. In the Rxo1-transgenic N. benthamiana, we …
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Development of a potential challenge model and plant-produced vaccine candidate for beak and feather disease virus
… expression in whole-plant Nicotiana benthamiana (N. benthamiana). Virus-like particles (VLPs) were purified and analysed using transmission electron microscopy. Virions from a Palm cockatoo (Probosciger aterrimus) were purified and a BFDV dsDNA molecular clone was synthesised and …
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BEYOND ARABIDOPSIS: UNRAVELLING THE HIDDEN WORLD OF CA2+ SIGNALLING ACROSS SPECIES VIA MULTISCALE IMAGING TECHNOLOGIES
… larger than Arabidopsis; iii) Use of a Nicotiana benthamiana (N. benthamiana) line expressing the GCaMP3 Ca2+ indicator as an alternative model to Arabidopsis for studying local and long-distance Ca2+ signalling; iv) Generation of a N. benthamiana line expressing an L-Glu indicator, to monitor in …
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Estudo dos mecanismos envolvidos na adaptabilidade diferencial de dois begomovírus em tomateiro e Nicotiana benthamiana
… induzidos pelo ToYSV em tomateiro e Nicotiana benthamiana são mais severos e surgem mais cedo em comparação aos induzidos pelo ToRMV, o que sugere uma adaptação diferencial de cada vírus aos seus hospedeiros. Neste trabalho, foram conduzidos experimentos com o objetivo de analisar o papel de …
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Bioproduction Optimization and Characterization of Hyaluronic Acid in Escherichia coli and Nicotiana benthamiana
… gene transfer to transform Nicotiana benthamiana with hHAS2. To enhance precursor availability, N. benthamiana was further engineered to co-express glmS and glmU, which encode key enzymes involved in HA biosynthesis. Co-expression of these genes resulted in a substantial increase in HA …
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Plant production of Gonococcal peptide vaccine, candidate peptide display with HPVs
… against HPV16 and gonorrhea, utilizing N. benthamiana as the expression host. The vaccine design involves the incorporation of a gonorrhea peptide, mimicking the 2C7 epitope of the surface molecule LOS on gonorrhea, into the surface DE loop of HPV16 virus-like particles (VLPs). These …
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INTERACTOMICS FOR RICE FLOWERING: A PROXIMITY LABELLING APPROACH
… un efficiente protocollo di PL su foglie di N. benthamiana agroinfiltrate e in piante di riso transgeniche che esprimono le suddette proteine di fusione. Le analisi preliminari su Hd1-TbID e FTL1-TbID hanno confermato la loro stabilità ed efficienza enzimatica. Successivamente, l’intero …
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Exploring Non-transgenic CRISPR-Cas9 gRNA Delivery Using Transactivation in Nicotiana benthamiana
… reporter expression. Transient assays in N. benthamiana established the functionality of the system, showing increased reporter levels when the two constructs were co-expressed compared to the activator- reporter construct alone. Stable transgenic N. benthamiana lines of the separate …
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Influenza hemagglutinin expression in Nicotiana tabacum and Nicotiana benthamiana.
… plasmid and both Nicotiana tabacum or Nicotiana benthamiana were transformed. To screen for HA3 expression, mRNA was isolated from first generation plants. Second generation plants were successfully screened for protein production using immunoblots to verify HA3 protein expression. A tobacco …
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Análisis de las interacciones de la proteína CI del virus de la sharka con proteínas de la planta
… híbridos de una genoteca de cDNA de Nicotiana benthamiana utilizando la proteína CI del virus de la sharka (PPV) como cebo. Se identificaron dos proteínas (L1 y L2) que interaccionaban específicamente con la proteína CI de PPV. L1 es una proteína desconocida con un dominio de dedos de zinc de …
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Molecular characterisation of the <i>Rhynchosporium commune</i> interaction with barley
… R. commune on the model dicotyledonous plant N. benthamiana and analysis of effector transcription during growth on a non-host. R. commune growth was shown to be confined to the leaf surface, with no evidence of plant cell deterioration in transgenic N. benthamiana plants expressing an …
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Characterization of grapevine vein clearing virus expression strategy and development of caulimovirus infectious clones
… The co-agroinfiltration assay of Nicotiana benthamiana developed in this chapter was used to evaluate the infectivity of an infectious clone of GVCV. Chapter 4 describes the construction of a terminally redundant clone of GVCV. The GVCV genome was assembled via three overlapping DNA …
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Identification and molecular characterisation of elicitor-priming for resistance in tomato against <i>Botrytis cinerea</i>
… crops <i>Solanum melongena</i>, <i>Nicotiana benthamiana</i>, <i>Solanum lycopersicum </i>and brassicaceous plant <i>Arabidopsis thaliana</i> by significantly decreasing necrotic lesion sizes and priming for callose deposition in a concentration-dependent manner. Furthermore, large-scale …
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Functional analysis of soybean dirigent genes
… and disease assays were performed in Nicotiana benthamiana and soybean, showing possible reduction in the size of lesions induced by Sclerotinia sclerotiorum when the soybean dirigent-like genes were transiently expressed in N. benthamiana.
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Functional Characterization of Four Xanthomonas euvesicatoria Type III Effectors
… elicit the hypersensitive response in N. benthamiana in the Agrobacterium-mediated transient assay and Xe-mediated disease assay. The interaction is confirmed by bimolecular fluorescence complementation, co-immunoprecipitation and split luciferase assay. Intriguingly, we also revealed that …
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The role of terpenes as protectors of the potato crop
… in potato and have been expressed in N. benthamiana to determine their function and possible involvement in Solanoeclepin A biosynthesis. Major potato crop losses are also caused by the onset of sprouting during storage. Potatoes are harvested in September, but must be stored for up to …
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