Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"N-terminal extension"”.
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The oxidative folding of insulin-like growth factor-I analogues / by Steven John Milner.
… investigates the effect of mutations and an N-terminal extension on the oxidative folding pathway of IGF-I, analyses the structure of the stable mis-folded molecule in terms of its biological interactions, examines the kinetics of the late stages of oxidative folding and finally attempts to …
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The Role of The Diras Family Members In Regulating Ras Function, Cancer Growth and Autophagy
… Ras family members by a 34-amino acid N-terminal extension that is required for its tumor suppressive function in ovarian cancer. By assessing the structure-function relationship, I found that DIRAS3 inhibits Ras-induced transformation and is a natural antagonist of Ras/MAPK signaling. …
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Insights into the role and mechanism of the AAA+ adaptor Clps
… ClpS in Escherichia coli. ClpS binds to the N-terminal domain of ClpA and plays dual roles in ClpAP substrate selectivity: ClpS inhibits the degradation of some substrates such as ssrA-tagged proteins and enhances the degradation of other substrates such as N-end-rule proteins. We wished to …
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Replication fidelity in the mircroevolution of mycobacteria
… on the inferred role of a highly-conserved N-terminal extension and C-terminal pentapeptide motif in the function of the alternative, error-prone DNA PolIIIα subunit, DnaE2.
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Structure-Function Studies of CorA-Mrs2-Alr1 Superfamily of Magnesium Channels: Bacterial CorA and Yeast Alr1
… of the CorA-Mrs2-Alr1 superfamily by a long N-terminal extension preceding the CorA domain. Bioinformatic analysis indicates that the N-terminal extension is mostly unstructured and contains multiple molecular recognition and phosphorylation sites, suggesting that it may participate in …
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Towards unveiling the Photoactive Yellow Proteins: characterization of a halophilic member and a proteomic approach to study light responses
… ruber contains an unusual 31-residue N-terminal extension which appears to be disordered relative to the remainder of the protein and which induces a unique dimerization of the photosensor with no precedents described before with any of the other published PYPs. Truncation of the …
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Comparative biochemistry and genetic analysis of nucleoside hydrolase in Escherichia coli, Pseudomonas aeruginosa, and Pseudomonas fluorescens.
… nucleoside hydrolase were found to contain an N-terminal extension of 30-35 amino acids that is shown to act as a periplasmic-signaling sequence. These are the first two nucleoside hydrolases, to date,that have been conclusively demonstrated to be exported to the periplasmic space. The …
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Contribution of troponin-I to the regulation of cardiac muscle
… isoform of TnI (cTnI) has a ~30 residue N-terminal extension, which contains two serines (Ser22/23) that become phosphorylated by protein kinase A upon \(\beta\)-adrenergic stimulation. However, the function of the N-terminus of cTnI remains unclear. Questions also remain about the function …
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Nucleoid-Associated Proteins of Streptomyces coelicolor: Discovery and Functions
… It was found to comprise a KH domain with an N-terminal extension and formed oligomers of 10 - 12 subunits in solution. The mutant phenotypes of both S. coelicolor paralogs of HU (HupA and HupS) were examined and showed opposite effects on growth, spore formation and actinorhodin production. The …
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Mechanism of active substrate delivery by the AAA+ protease adaptor CIpS
… Here, we demonstrate that the unstructured N-terminal extension (NTE) of CIpS enters the CIpA processing pore in the active ternary complex and that CIpA engagement of the CIpS NTE is crucial for CIpS-mediated substrate delivery. In addition, I report evidence that CIpA engagement of the CIpS …
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Biochemical Characterization of Two Aminopeptidases Involved in Hemoglobin Catabolism in the Food Vacuole of Plasmodium falciparum
… to be associated with the Plasmodium specific N-terminal extension found in the PfA-M1 sequence by yellow fluorescent protein fusion studies. Kinetic analysis of recombinant forms of PfAPP and PfA-M1 revealed both enzymes are stable and catalytically efficient in the substrate rich, acidic …
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Mechanisms of CipS reprogramming of the AAA+ protease CipAP
… I demonstrate that the unstructured N-terminal extension of ClpS is both necessary and sufficient to inhibit substrate degradation. Engagement of this NTE by the ClpA translocation pore is critical for CIpS-mediated inhibition. I propose a model where ClpS behaves as a substrate mimic …
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Regulation of γ-Tubulin Ring Complex Recruitment in Drosophila
… In contrast, the absence of the inhibitory N-terminal extension within the testes-specific Cnn isoforms allows these isoforms to bind γ-TuRCs at mitochondria, which lack mitotic kinases. This would provide cycling cells with control over when and where their γ-TuRCs are activated, contributing …
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Regulation of γ-Tubulin Ring Complex Recruitment in Drosophila
… In contrast, the absence of the inhibitory N-terminal extension within the testes-specific Cnn isoforms allows these isoforms to bind γ-TuRCs at mitochondria, which lack mitotic kinases. This would provide cycling cells with control over when and where their γ-TuRCs are activated, contributing …
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Replacement of Val3 In Human Thymidylate Synthase Affects Its Kinetic Properties and Intracellular Stability, Complexes of Sperm Whale Myoglobin G65T With Phenol and Ethylene Glycol, and Structure of A Fragment of Human End-Binding Protein 1 (Eb1)
… thymidylate synthase (TS) enzymes have an N-terminal extension of about 27 amino acids which is not present in bacterial TSs. The extension has been considered to play a primary role in protein turnover but not in catalytic activity. Two mutants, V3L and V3F, have strongly compromised dUMP …
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Genetic and Biochemical Studies of YqjH and YqjI: Cross-talk between Iron Homeostasis and Nickel Homeostasis in Escherichia Coli
… regulators and also contains an N-terminal extension similar to the Ni2+-binding C-terminal tail of SlyD. We found that YqjH has ferric reductase activity and is required for iron homeostasis in E. coli. In addition, elevated nickel stress levels disrupt iron homeostasis in E. coli …
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The N-end rule degradation pathway : substrate recognition and staged delivery to the CIpAP protease by the CIpS adaptor protein
… protein stability to the identity of its N-terminal residue in both prokaryotes and eukaryotes. For example, in bacteria, Tyr, Phe, Trp, and Leu serve as degradation signals when located at the N-terminus of a protein. In E. coli, the bacterial adaptor CIpS recognizes these signals and …
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Spectroscopic investigation of phytochromes and NIR fluorescent variants
… in plant phytochromes is affected i.a. by the N-terminal extension and binding of PIF proteins (phytochrome interaction factors), which in turn modulates the lifetime of the Pfr state. Furthermore, the photosensor constructs with and without the PHY domain (PAS-GAF-PHY [PGP], PAS-GAF [PG]) were …
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In vitro-Untersuchungen zum Sekretionsmechanismus von Pathogenitätsfaktoren mit einer N-terminalen Extension in Escherichia coli
… innerhalb der Signalsequenz, sowie durch eine N-terminale Extension, die der Signalsequenz am N-terminalen Ende vorausgeht, auszeichnen. <br>In dieser Arbeit wurde unter Verwendung eines homologen Transkriptions-Translationssystems von E. coli erstmalig der Sekretionsweg dieser Proteine am …
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Channel-forming proteins in the cell wall of amino acid-producing Corynebacteria
… PorHC.eff and PorHC.glut. The proteins have no N-terminal extension, only the inducer methionine, which suggests that secretion of the proteins could be very similar to that of PorAC.glut of C. glutamicum. PorHC.glut is coded in the bacterial chromosome by a gene that is localized in the vincinity …