Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"N-linked glycosylation"”.
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Priming and processing glycosyltransferases in bacterial N-linked glycosylation pathways
… In the final chapter, the enzymes in the N-linked protein glycosylation pathway in Campylobacter jejuni were evaluated for their tolerance for azide-modified UDP-sugar substrates. In vitro experiments were employed to investigate the potential of these enzymes for executing the …
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Biochemical and biophysical investigations of N-linked glycosylation pathways in archaea
Asparagine-linked glycosylation is an abundant and complex protein modification conserved among all three domains of life. Much is known about N-glycan assembly in eukaryotes and selected bacteria, in which the oligosaccharyltransferase (OTase) carries out the en bloc transfer of glycans from …
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N-linked glycosylation is fundamentally linked to the surface expression of neuroligins
N-linked glycosylation is one of the most prevalent forms of post-translational modification, decorating secreted and cell-surface transmembrane proteins as they are trafficked along the secretory pathway. While well-characterized in most tissues, non-canonical N-glycan diversification has been …
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Modifying N-linked glycosylation in tobacco to produce human-type post-translational modifications
… mammalian cells, most critically in terms of N-linked glycosylation. This can impact both protein functionality and stability, key for human therapeutics intended for production in plants. This obstacle can be approached by creating a plant-based system capable of “humanizing” proteins of …
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Effects of variation in N-linked glycosylation site on antibody mediated neutralization in PRRSV
… epitope which is flanked by three N-linked glycosylation sites, N34, N44 and N51. It is believed that these glycosylation sites play a role in glycan shielding which is a primary mechanism by which the virus evades neutralizing immune responses. Analysis of GP5 sequences of a subset …
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The importance of N-linked glycosylation on the N-domain of angiotensin-I converting enzyme
… catalytically active and heavily glycosylated. Glycosylation is one of the most important forms of post-translational modification, having a wide range of functions including protein folding, modulation of the immune response, and providing targeting signals. Glycosylation is required for the …
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The role of N-linked glycosylation on the structure and function of somatic angiotensin-converting enzyme
… and C-domain), both of which are glycosylated. N-linked glycosylation is important for the processing, expression and stability of ACE, but it interferes with protein crystallization. Previously, the N-glycan site occupancy required for the expression and stability of the individual domains of ACE …
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Modeling congenital disorders of glycosylation in Caenorhabditis elegans: Genetic influences and structural consequences of N-linked glycosylation
… in all metazoans. This process, known as N-glycosylation, is complex and is achieved by the precise interactions of various cellular components. The initial stage of N-glycosylation occurs in the endolasmic reticulum and is preserved among eukaryotes. Glycans are further developed in the …
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N-linked glycosylation of ether á go-go potassium channels: effects on cell surface expression and functional properties
Ether á go-go (Eag1) ist ein spannungsabhängiger Kaliumkanal, der offenbar an einer Vielzahl von zellulären Prozessen beteiligt ist. Die Ergebnisse dieser Arbeit zeigen, dass das Protein Eag1 aus Rattengewebe vorwiegend im Gehirn exprimiert wird. Dort unterliegt es der N-Glykosylierung, einer …
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N-linked glycosylation at position ASN98 of the ALK1 receptor protein: relevance for ALK1 function and HHT pathogenesis
… My thesis research was aimed at studying the glycosylation of ALK1 and at exploring the relevance of this glycosylation to the development of HHT. The glycosylation of ALK1 was investigated by using: (i) a computational prediction approach (NetNGlyc 1.0 bioinformatics server), (ii) a …
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The role of n-linked glycosylation and sialylation in regulating human proteinase-activated receptor 1 and 4 expression and signalling
… effects of proteinases on target cells. N-linked glycosylation is a common post-translational modification in the GPCR superfamily that enables the cell to regulate protein function without recourse to the genome. Previous pharmacological studies have found that activation of hPAR-2 by mast …
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Investigating asparagine-linked glycosylation substrate : specificity and effects on protein folding
N-linked glycosylation is a ubiquitous form of protein modification whereby a preassembled oligosaccharide is covalently attached the asparagine side chain of an acceptor protein. This process involves numerous enzymes, produces a diverse set of oligosaccharide structures, and results in a variety …
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Glycan Shielding of the Influenza Virus Hemagglutinin Elicits Evasion of the Adaptive Immune Response and T-Cell-Driven Pathology
… 1918, each characterized by viruses that lack N-linked glycosylation sites on the globular head of the hemagglutinin protein. In contrast, recent non-pandemic isolates have acquired such sites. Here we constructed isogenic viruses containing differing numbers of additional N-linked glycosylation …
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Investigation of the structure requirements for oligosaccharyl transferase function
N-linked glycosylation is a ubiquitous protein modification involved in a wide range of cellular functions and diseases (Varki, 1993). The oligosaccharyl transferase (OTase), the principle enzyme responsible for catalysis of N-linked glycosylation, facilitates the transfer of a preassembled …
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Biogenesis and Traffic of the Potassium Channel
… integration into the lipid bilayer, (ii) N-linked glycosylation, assembly and folding Shaker in the ER, and (iii) export of Shaker from the ER and subsequent traffic to the surface. Targeting to the endoplasmic reticulum, integration into the lipid bilayer and assembly into tetramers occurs …
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Mechanisms of attenuation of the live Junin virus vaccine strain, Candid #1
… within infected cells. Candid #1 loses an N-linked glycosylation motif prior to the 44th mouse brain passage of the XJ strain, which coincides with a significant decrease in fully processed and cleaved glycoprotein complexes (GPCs) and a significant increase in full-length GP. JUNV GPs in …
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Investigation of Swine Influenza Virus Genetic Diversity and Evolution
… strains (chapter 3). N144 lies in a predicted Nlinked glycosylation consensus sequence. Interestingly, neutralization-sensitive viruses all had predicted N-linked glycosylation sites at N142 of HA. Serological assays demonstrated that migration of the potential N-linked glycosylation from N142 …
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Structural underpinnings of membrane association and mechanism in the monotopic phosphoglycosyl transferase superfamily
In prokaryotes, protein glycosylation can be a determinant of pathogenicity as it plays a role in host adherence, invasion, and colonization. Impairment of glycosylation in some organisms, for example N-linked glycosylation in Campylobacter jejuni, leads to decreased pathogenicity; thus, opening …
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Investigating asparagine-linked protein glycosylation in eukaryotic and prokaryotic systems
N-linked protein glycosylation is characterized by the formation of a -glycosylamine linkage to an asparagine residue within the Asn-Xaa-Ser/Thr consensus sequence. This modification is found in organisms from eukaryotic, archaeal and bacterial domains and is implicated in numerous cellular …
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Protein expression and glycosylation in CHO cells
… depends on sufficient expression and correct glycosylation of the recombinant product. Low product titer and inconsistent protein glycosylation constitute two major problems frequently encountered in biopharmaceutical production. Using Chinese Hamster Ovary (CHO) cells expressing recombinant …
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