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Showing 1 to 20 of 88 for “"N-linked"”.

  1. Priming and processing glycosyltransferases in bacterial N-linked glycosylation pathways

    Lukose, VinitaMassachusetts Institute of Technology2015

    … In the final chapter, the enzymes in the N-linked protein glycosylation pathway in Campylobacter jejuni were evaluated for their tolerance for azide-modified UDP-sugar substrates. In vitro experiments were employed to investigate the potential of these enzymes for executing the …

    mit Repository record

  2. Biochemical and biophysical investigations of N-linked glycosylation pathways in archaea

    Chang, Michelle M. (Michelle Miran)Massachusetts Institute of Technology2015

    Asparagine-linked glycosylation is an abundant and complex protein modification conserved among all three domains of life. Much is known about N-glycan assembly in eukaryotes and selected bacteria, in which the oligosaccharyltransferase (OTase) carries out the en bloc transfer of glycans from …

    mit Repository record

  3. N-linked glycosylation is fundamentally linked to the surface expression of neuroligins

    Cast, Thomas, author, Chanda, Soham, advisor, DeLuca, Jennifer, committee member, Di Pietro, Santiago, committee member, Tobet, Stuart, committee memberColorado State University. Libraries2023

    N-linked glycosylation is one of the most prevalent forms of post-translational modification, decorating secreted and cell-surface transmembrane proteins as they are trafficked along the secretory pathway. While well-characterized in most tissues, non-canonical N-glycan diversification has been …

    colostate Repository record

  4. Uncovering unique N-linked glycan structural isomers in cancer via MS(n) disassembly

    Prien, Justin Michael

    … systematically uncovers an anomalous set of N-linked glycans distinct to malignant non-metastatic and metastatic tumors. By coupling specific chemical workup and utilizing ion-trap MSn disassembly, this analytical approach introduces a concept that carbohydrate epitopes can serve as a sensitive …

    unh-thes Repository record

  5. Modifying N-linked glycosylation in tobacco to produce human-type post-translational modifications

    McGinness, AlastairOxford Brookes University

    … mammalian cells, most critically in terms of N-linked glycosylation. This can impact both protein functionality and stability, key for human therapeutics intended for production in plants. This obstacle can be approached by creating a plant-based system capable of “humanizing” proteins of …

    oxford-brookes Repository record

  6. Effects of variation in N-linked glycosylation site on antibody mediated neutralization in PRRSV

    Misra, AnishaUniversity of Illinois at Urbana-Champaign2012

    … epitope which is flanked by three N-linked glycosylation sites, N34, N44 and N51. It is believed that these glycosylation sites play a role in glycan shielding which is a primary mechanism by which the virus evades neutralizing immune responses. Analysis of GP5 sequences of a subset …

    uiuc Repository record

  7. Modulating the Antigenicity and Immunogenicity of HIV-1 Envelope by a Conserved N-Linked Glycan

    Townsley, Samantha2016

    … that removal of a highly conserved potential N-linked glycan (PNLG) site at amino acid residue 197 (N7) on the surface antigen gp120 of HIV-1 envelope glycoprotein (Env) increases neutralization sensitivity of the mutant virus to CD4 binding site (CD4bs)-directed antibodies and enhanced the …

    washington Repository record

  8. The importance of N-linked glycosylation on the N-domain of angiotensin-I converting enzyme

    Anthony, Colin ScottDivision of Medical Biochemistry2011

    … is known about the degree and importance of N-linked glycosylation on the N-domain. The generation of minimally glycosylated N-domain, however, requires a greater understanding of the relative importance of the individual N-linked glycosylation sites.

    cape-town Repository record

  9. Analysis of N-Linked Oligosaccharides of Prostate-Specific Antigen and Prostatic Acid Phosphatase in Prostatic Fluids

    White, Krista Yaudes2019

    <p>Presently, prostate cancer is the most common cancer afflicting men in the United States, with serum PSA being the "gold standard" protein biomarker used in the clinic for detecting and diagnosing prostate cancer. Nonetheless, serum PSA levels can also be elevated in non-cancerous conditions as …

    odu Repository record

  10. The role of N-linked glycosylation on the structure and function of somatic angiotensin-converting enzyme

    Nkoe, Karabelo MDepartment of Medicine2014

    … and C-domain), both of which are glycosylated. N-linked glycosylation is important for the processing, expression and stability of ACE, but it interferes with protein crystallization. Previously, the N-glycan site occupancy required for the expression and stability of the individual domains of ACE …

    cape-town Repository record

  11. Modeling congenital disorders of glycosylation in Caenorhabditis elegans: Genetic influences and structural consequences of N-linked glycosylation

    Struwe, Weston Booth

    <p>The attachment of oligosaccharides to the amide nitrogen of asparagine side chains in proteins is a fundamental process occurring in all metazoans. This process, known as N-glycosylation, is complex and is achieved by the precise interactions of various cellular components. The initial stage of …

    unh-thes Repository record

  12. N-linked glycosylation of ether á go-go potassium channels: effects on cell surface expression and functional properties

    Napp, Joanna2012

    Ether á go-go (Eag1) ist ein spannungsabhängiger Kaliumkanal, der offenbar an einer Vielzahl von zellulären Prozessen beteiligt ist. Die Ergebnisse dieser Arbeit zeigen, dass das Protein Eag1 aus Rattengewebe vorwiegend im Gehirn exprimiert wird. Dort unterliegt es der N-Glykosylierung, einer …

    goettingen Repository record

  13. N-linked glycosylation at position ASN98 of the ALK1 receptor protein: relevance for ALK1 function and HHT pathogenesis

    Gadaleta, Erick Michael2016

    … bioinformatics software NetNGlyc predicted a N-linked glycosylation site on an asparagine (ASN) residue located at position 98 in the extracellular domain of ALK1. I further found that, based on western blot analysis, ALK1 proteins shifted to a lighter molecular weight (5-8 kDa) when treated …

    bu Repository record

  14. The role of n-linked glycosylation and sialylation in regulating human proteinase-activated receptor 1 and 4 expression and signalling

    Xiao, Yu PeiUniversity of Hull2008

    … effects of proteinases on target cells. N-linked glycosylation is a common post-translational modification in the GPCR superfamily that enables the cell to regulate protein function without recourse to the genome. Previous pharmacological studies have found that activation of hPAR-2 by mast …

    hull Repository record

  15. Investigating asparagine-linked glycosylation substrate : specificity and effects on protein folding

    Chen, Mark MMassachusetts Institute of Technology2009

    N-linked glycosylation is a ubiquitous form of protein modification whereby a preassembled oligosaccharide is covalently attached the asparagine side chain of an acceptor protein. This process involves numerous enzymes, produces a diverse set of oligosaccharide structures, and results in a variety …

    mit Repository record

  16. Glycan Shielding of the Influenza Virus Hemagglutinin Elicits Evasion of the Adaptive Immune Response and T-Cell-Driven Pathology

    Wanzeck, Keith C.2016

    … 1918, each characterized by viruses that lack N-linked glycosylation sites on the globular head of the hemagglutinin protein. In contrast, recent non-pandemic isolates have acquired such sites. Here we constructed isogenic viruses containing differing numbers of additional N-linked glycosylation …

    tenn-hsc Repository record

  17. Investigation of the structure requirements for oligosaccharyl transferase function

    Jaffee, Marcie B. (Marcie Beth)Massachusetts Institute of Technology2013

    N-linked glycosylation is a ubiquitous protein modification involved in a wide range of cellular functions and diseases (Varki, 1993). The oligosaccharyl transferase (OTase), the principle enzyme responsible for catalysis of N-linked glycosylation, facilitates the transfer of a preassembled …

    mit Repository record

  18. Investigating asparagine-linked protein glycosylation in eukaryotic and prokaryotic systems

    Weerapana, EranthieMassachusetts Institute of Technology2006

    N-linked protein glycosylation is characterized by the formation of a -glycosylamine linkage to an asparagine residue within the Asn-Xaa-Ser/Thr consensus sequence. This modification is found in organisms from eukaryotic, archaeal and bacterial domains and is implicated in numerous cellular …

    mit Repository record

  19. Effects of glycosylation on the structure and fibrillization of prion protein fragments

    Bosques, Carlos J. (Carlos Javier), 1974-Massachusetts Institute of Technology2003

    … the Imperiali group has previously shown that N-linked glycosylation can play a major role modulating polypeptide conformation. It has also been shown that glycosylation can alter the thermodynamics of disulfide bond formation, favoring oxidation. To address the role of glycosylation on PrP, we …

    mit Repository record

  20. Analysis and characterisation of human chorionic gonadotropin glycoforms in pregnancy and trophoblastic disorders

    Gondek, M.Middlesex University2022

    … to permit the characterisation of hCG N-linked glycans from urine samples collected throughout normal pregnancy and GTD using matrix-assisted laser desorption ionisation time of flight mass spectrometry (MALDI-TOF MS). hCG isolated from pooled pregnancy urine was used in this study. All …

    middlesex Repository record

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