Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 49 for “"N-glycosylation"”.
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Complex N-Glycosylation in Anti-Tumor Immunity
… anti-tumor target for two reasons: (i) protein glycosylation is known to play a role in tumor progression, and (ii) alternatively glycosylated proteins may function as tumor neoantigens. The glycosyltransferase Mgat5 catalyzes the formation of 1,6-N-acetylglucosamine branched glycans, and …
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Impaired N-Glycosylation Processing Impacts Neuronal Function
… are carried out primarily using N-linked glycosylation. N-glycosylation has the potential to co-translationally and post-translationally modify protein function by the addition and processing of oligosaccharides. These oligosaccharides are known as glycans and are broken up into three …
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N-Glycosylation Protects Host from Mycobacterial Infection
… screen, we have identified the early steps of N-glycosylation are required for the macrophage to resist mycobacterial infections. N-glycosylation attaches an oligosaccharide molecule to nascent secretory or membrane proteins within the endoplasmic reticulum (ER) for protein folding and …
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Engineered Oligosaccharyltransferases With Unique N-Glycosylation Site Preferences
The key enzyme in the Campylobacter jejuni glycosylation pathway, PglB, has been one of the well-studied asparagine-linked oligosaccharyltransferase (OST) in bacterial glycoengineering area. While C. jejuni PglB performs well in transferring different glycans, the acceptor sites it can recognize …
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N-Glycosylation profiles as a risk stratification biomarker for Type II Diabetes Mellitus and its associated factors
… of oligosaccharides (glycans) to proteins-glycosylation is of metabolic and physiological significance, as exploring aberrations of glycosylation profiles can reveal novel biomarkers. In parallel, this process could also explain the biological mechanisms that underpin a suboptimal health …
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Modification of a plant-based expression system to produce Lysosomal Acid Lipase (LAL) in plant cells using genetic modification of endogenous plant N-glycosylation
As plant cells are capable of complex N-glycosylation they are a valid expression system to produce safe and correctly glycosylated human proteins. To modify plant glycosylation patterns towards fully human patterns, the plant glycosylation machinery requires substitution of a series of plant …
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The impact of HIV-1 subtype C Envelope N-glycosylation on DC-SIGN meditated modulation of DC function to facilitate transmission or enhance viral pathogenesis
N-glycosylation plays an important role in Envelope (Env) function and may be involved in the modulation of the immune response to HIV-1 infection. In this study, we hypothesized that Env N-glycosylation may affect viral pathogenesis by influencing Env structure and function. Furthermore, we also …
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IMPROVING GLYCOSYLATION EFFICIENCY IN ESCHERICHIA COLI BY MAMMALIAN-CELL-INSPIRED PROTEIN-PROTEIN INTERACTIONS
Asparagine linked glycosylation (N-glycosylation) is one of the most common post-translational modifications found in proteins. The central enzyme in the N-glycosylation pathway responsible for the transfer of glycans onto asparagine residues is called the oligosaccharyltransferase (OST). In …
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Modeling congenital disorders of glycosylation in Caenorhabditis elegans: Genetic influences and structural consequences of N-linked glycosylation
… in all metazoans. This process, known as N-glycosylation, is complex and is achieved by the precise interactions of various cellular components. The initial stage of N-glycosylation occurs in the endolasmic reticulum and is preserved among eukaryotes. Glycans are further developed in the …
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Mass Spectrometric Analysis of Tyrosine Metabolic Enzymes
… and MS analysis of DCE and DCT determined that N-glycosylation is a primary post-translational modification. Q-TOF mass spectrometry was used to determine N-glycosylation patterns from Aedes aegypti DCE and MALDI-TOF/TOF was used to determine multiple glycosylation sites in DCT. N-glycosylation is …
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Synthesis Of Functionalized Gpi Anchors And Related Glycoconjugates
… 12-amino acid glycopeptide containing a single N-glycosylation site, to which complex glycans are attached. This glycoconjugate exists in two distinct functional forms, including lymphocyte and sperm CD52, which are involved in the human immune and reproductive systems, respectively. The …
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Improving reproducibility and standards in quantitative N-Glycoproteomic data
… of all human proteins are glycosylated, making glycosylation one of the most abundant post-translational modifications in proteomics. N-Glycosylation is a prevalent and diverse type of glycosylation with key roles in regulating systems such as protein folding and host-pathogen recognition; …
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Kinetics and regulation of the initial stages of cadherin-mediated adhesion
… to the protein backbone in a process called glycosylation. The effect that one type of glycosylation, N-glycosylation, has on cadherin kinetics was investigated. It was determined that the loss of N-glycosylation on regions of the protein not associated with the Tryptophan strand exchange can …
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Structural analysis of cytokine signaling modulators
… N-glycan profiling showed alterations in N glycosylation generated by GlcNAc supplements through stimulation of hexosamine pathway. After methylation the tetra-antennary and poly-N-acetyl lactosaminylated glycans were identified only in Mgat 5 wild type cells either supplemented or not with …
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Small Molecule Regulator of ENTPD5, and ER Enzyme in the PTEN/AKT Pathway
… of ENTPD5 in PTEN-null MEF cells accelerates glycosylation substrate replenishment, therefore promotes N-glycosylation and increases ER protein folding capacity to accommendate the increase of protein synthesis resulted from active PI3K/Akt signaling. Knockdown of ENTPD5 in PTEN-null cells …
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Slc39a8/zip8 Influences Complex Traits By Regulating Metal Ion Metabolism
… Mn. Slc39a8 deletion in mice leads to protein N-glycosylation defects indicative of decreased activity of a Mn-dependent enzyme, β-1, 4-galactosyltransferase, while homozygosity of a SLC39A8 loss-of-function variant is associated with hypogalactosylation, suggesting that protein N-glycosylation …
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Molecular characterization of cation-coupled transporters: the H+-coupled Mg2+-citrate transporter, CitM, and the Na+/sulfate cotransporter, hNaSi-1
… used to investigate tissue distribution and N-glycosylation. The roles of two conserved serine residues in the transport function of hNaSi-1 were investigated using site-directed mutagenesis and radiotracer assay. \r\n\r\n CitM belongs to a distinct gene family of secondary active transporters …
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Regulation of cadherin adhesion at intercellular junctions
… investigated the effect of N-linked and O-linked glycosylation on E-cadherin activity and function. Results revealed that, contrary to the influence of glycosylation on N-cadherin function, N-glycosylation of E-cadherin in the EC4 and EC5 domains negatively regulated cadherin adhesion, by altering …
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The importance of N-linked glycosylation on the N-domain of angiotensin-I converting enzyme
… catalytically active and heavily glycosylated. Glycosylation is one of the most important forms of post-translational modification, having a wide range of functions including protein folding, modulation of the immune response, and providing targeting signals. Glycosylation is required for the …
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The role of N-linked glycosylation on the structure and function of somatic angiotensin-converting enzyme
… both of which are glycosylated. N-linked glycosylation is important for the processing, expression and stability of ACE, but it interferes with protein crystallization. Previously, the N-glycan site occupancy required for the expression and stability of the individual domains of ACE was …
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