Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"N-glycans"”.
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Harnessing N-glycans as Next Generation Prebiotics
N-linked glycans are structurally diverse carbohydrate moieties that are covalently boundto dietary glycoproteins. Upon ingestion, N-glycans from dietary glycoproteins escape absorption in the small intestine and travel to the colon for fermentation. Ample evidence has indicated that certain …
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Emerging out of the “blur”: exploration, evaluation and significance of 3D N-glycans’ structure through molecular dynamic studies
… stability and dynamics, and function. Complex N-glycans are a class of glycans found in eukaryotes, sharing a common pentasaccharide core structure. The functionalization of the arms and the branching patterns are specific to different species, while the complexity of the cellular biosynthetic …
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Regulation of receptor signaling and membrane trafficking by beta1,6-branched n-glycans and caveolin-1/cholesterol membrane domain organization
… reflecting their structural variability. N-glycans regulate many biological outcomes in mammalian cells under both normal and pathological conditions. They play a major role in various pathologies such as cancer and lysosomal storage diseases. Interplay between N-glycans and other …
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Analysis and characterisation of human chorionic gonadotropin glycoforms in pregnancy and trophoblastic disorders
… to permit the characterisation of hCG N-linked glycans from urine samples collected throughout normal pregnancy and GTD using matrix-assisted laser desorption ionisation time of flight mass spectrometry (MALDI-TOF MS). hCG isolated from pooled pregnancy urine was used in this study. All the …
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Distant chaperones and N-glycan signals : new mechanisms of secretory pathway proteostasis
… on specific asparagine residues. These N-glycans are installed in the ER as uniform precursors, but are trimmed and built up by Golgi glycan maturation enzymes into a striking array of epitopes. N-glycans act as a second mechanism to stabilize protein structure and prevent the release of …
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ELECTROKINETIC SEPARATIONS ON MICROFLUIDIC DEVICES FOR N-GLYCAN PROFILING
… and more efficient separation techniques. N-glycans are essential molecules in many living systems and may become important biomarkers for cancer and other diseases in humans. In this work, we developed several microfluidic platforms for separation and analysis of N-glycans. On microfluidic …
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The roles of modifiable risk factor and immunoglobulin G N-glycome in the risk of hearing loss
… loss with high levels of agalactosylated IgG N-glycans (prevalence ratio [PR] 1.25, 95% CI, 1.04-1.51) and asialylated N-glycans (PR 1.26, 95% CI, 1.05-1.51), whereas low levels of monosialylated N-glycans (PR 0.79, 95% CI, 0.66-0.97) and N-glycans with high-mannose glycoforms (PR 0.81, 95% CI, …
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Glycan characterizations with application in metal ion binding, the 1918 influenza virus neuraminidase formation and human receptors
… on the comprehensive characterization of its N-glycans. This included: (a) accurate mass measurement and GC/MS to determine the monosaccharide compositions; (b) positive ion CID-MS n to disassemble the deuterated permethylated derivates (CD3 ) to reveal the antenna sequence, and natural methoxyl …
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Development of novel analytical tools for complex carbohydrates
… such as the heparin/heparan sulfate glycosaminoglycans (HSGAGs) and the asparagine linked glycans (N-glycans) have emerged as crucial components of many human biological systems. Both classes of molecules have diverse roles in normal and pathological processes including development, cell growth, …
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Computational Studies of Glycan Conformations in Glycoproteins
N-glycans refer to oligosaccharide chains covalently attached to the side chain of asparagine (Asn) residues, and the majority of proteins synthesized in the endoplasmic reticulum (ER) are N-glycosylated. N-glycans can modulate the structural properties of proteins due to their close proximity to …
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Discovery of Glyco-biomarkers for Diabetes and Complications in Diabetes
… and diabetic plasma samples. Fucosylated N-glycans and intersected N-glycans were found to be up-regulated in diabetes with statistical significance. The elevated fucosylation in diabetes was verified at the glycosylation site level by quantitative Aleuria aurantia lectin (AAL) pull-down …
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N-Glycosylation profiles as a risk stratification biomarker for Type II Diabetes Mellitus and its associated factors
… The enzymatic attachment of oligosaccharides (glycans) to proteins-glycosylation is of metabolic and physiological significance, as exploring aberrations of glycosylation profiles can reveal novel biomarkers. In parallel, this process could also explain the biological mechanisms that underpin a …
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Expression and function of basigin during early pregnancy and spermatogenesis
… of N-acetylglucosamine (GlcNAc) terminated N-glycans that participate in germ cell-Sertoli cell adhesion. Results showed that Bsg KO spermatocytes displayed normal homologous chromosome synapsis and progression to the midpachytene stage. Both meiosis I (MI) and meiosis II (MII) spermatocytes …
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Manipulation of the Rift Valley Fever Virus M-Segment for Vaccine Development
… Gn and Gc, and determine the roles of the N-glycans in DC-SIGN-mediated infection. The results determined that the single N-glycan site within Gn, at nt. 438, was N-glycosylated and three sites within Gc, at nt. 794, 1035, and 1077; are N-glycosylated. We also noted that the doublet band of …
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Metabolic Pathways for Natural and Unnatural Sialic Acids
… results in changes in the branch structure of N-glycans and impairs galectin-1 binding to cells. Furthermore, I found that sialylation of N-glycans can further influence N-glycan branching. Taken together, my work suggests unexplored mechanisms for hIBM pathogenesis. The use of unnatural sialic …
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Structural analysis of cytokine signaling modulators
<p>The N-linked glycans attached to cytokine receptors modulate signal transduction by interacting with galectin-3 and generating a cell surface lattice. This opposes constitutive endocytosis and reduces the thresholds for cytokine signaling. The preferred ligands for galectin-3 are the …
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TUMOR-PROMOTING FUNCTION AND MOLECULAR REGULATION OF TSPAN8 IN LIVER CANCER
… We identified that modification of biantennary N-glycans and synthesis of lacto-series glycolipids mediated by a SPPL3/B3GNT5 axis are important for cell surface presentation of TSPAN8 and other tetraspanins. Collectively, our findings unmasked the potential of TSPAN8 as a diagnostic biomarker and …
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N-linked glycosylation is fundamentally linked to the surface expression of neuroligins
… reveal a profound overall significance of N-glycans for NLGNs and the conserved role of a specific N-linked glycosylation site for promoting the forward trafficking of NLGN protein.
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Complex N-Glycosylation in Anti-Tumor Immunity
… a lack of effective treatments. Tumor-associated glycans represent a potential anti-tumor target for two reasons: (i) protein glycosylation is known to play a role in tumor progression, and (ii) alternatively glycosylated proteins may function as tumor neoantigens. The glycosyltransferase Mgat5 …
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Chemical tools to probe carbohydrate recognition and glycoconjugate biosynthesis
… activities responsible for biosynthesis of N-glycans in Euglena gracilis, along with detection of N-acetylglucosamine-1-phosphate transferase activity. In a separate study, a series of linear and cyclic 1,4/1,5-triazole-linked-pseudo-galctooligomers were synthesised from …
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