Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 12 of 12 for “"N-end rule"”.
-
Substrate specificity of [alpha]-proteobacterial N-end rule adaptors
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 2016.
-
Tuning strength and specificity in the N-end rule
… in vivo, which led to a collection of N-end rule signals. The N-end rule describes how the identity of a protein's N-terminal residue determines its metabolic stability. In E. coli, CIpAP is the principal protease that degrades proteins bearing an N-terminal phenylalanine, tyrosine, …
-
Characterization of the Ubiquitin N-end Rule Pathway in Arabidopsis
… system. A subset of this system, termed the N-end rule pathway, relates the in vivo longevity of a substrate protein to the nature of its N-terminal amino acid. Although the N-end rule pathway is known to regulate numerous physiological processes in plants relatively few substrates of the …
-
The N-end rule degradation pathway : substrate recognition and staged delivery to the CIpAP protease by the CIpS adaptor protein
… ranging from bacteria to mammals, ATP-dependent proteases carry out regulated protein degradation in order to maintain homeostasis as well as to respond to stress. ATP-dependent proteases are responsible for the degradation of a broad array of substrates and for that reason, a high degree …
-
Insights into the role and mechanism of the AAA+ adaptor Clps
… in regulatory pathways. Intracellular ATP-dependent proteases are responsible for regulated degradation and are highly controlled in their function, especially with respect to substrate selectivity. Adaptor proteins that can associate with the proteases add an additional layer of control to …
-
Identification and generation of new germplasms in barley with increased tolerance to waterlogging
… has become an important research focus. The N-end rule protein degradation pathway has been shown to play an important role in the response of plants to flooding in Arabidopsis thaliana. Based on sequence similarities, N-end rule components were identified in barley and used as targets to …
-
Mechanism of active substrate delivery by the AAA+ protease adaptor CIpS
… in all organisms involves deployment of ATP-dependent proteases, consisting of hexameric AAA+ rings that unfold and translocate specific substrates into an associated peptidase barrel. Adaptor proteins assist in recognition and degradation of certain substrates, but how enzyme-adaptor pairs …
-
Signalwege und Toleranzfaktoren für die Anpassung an Hypoxie und Überflutung in Arabidopsis thaliana
Arabidopsis thaliana wurde in dieser Arbeit verwendet, um Signalwege und Anpassungsmechanismen zu untersuchen, die die Toleranz gegen Überflutung und Hypoxiestress beeinflussen. Eine Gruppe von 49 Genen wird bei Hypoxie in verschiedensten Arabidopsis-Zelltypen verstärkt exprimiert (Mustroph et al. …
-
Delivery of biomolecules into mammalian cells using anthrax toxin
… Efficient translocation and disruption of the intended PPI in each case indicated that the delivery platform could be used to deliver bioactive cargos into cells for therapeutic utility. As an application of this technology, the PA/LFN delivery platform was employed to analyze the intracellular …
-
Une approche afin de produire les différentes conformations de caspase-7 tout en contrôlant l'induction de l'apoptose
… the stability of caspase-7 according to the N-end rule, which relates the half-life of a protein with the residue presented at its N-terminus. This degradation pathway controls the ubiquitination of the protein based on the N-terminus. To replicate the different forms of caspase-7 produced …
-
Physiological and Molecular Dissection of Salinity Tolerance in Arabidopsis and Maize and Nitrogen Uptake in Wheat
The PROTEOLYSIS 6 (PRT6) branch of the N-end rule pathway is a well-characterized negative regulator of flooding and low oxygen tolerance in plants. This study investigated the role of this pathway in adaptation to salinity stress in Arabidopsis and maize via physiological and molecular …
-
Zentrale Cis- und Transregulation der pflanzlichen Hypoxieantwort
… zum Beispiel nach starken Regenfällen, während Staunässe oder Überflutung unter den Km-Wert der Cytochrom-c-Oxidase, erfährt die Pflanze eine Energiekrise durch ATP-Mangel. Um dem entgegenzuwirken, haben Pflanzen Merkmale entwickelt, die die Verfügbarkeit von Sauerstoff entweder erhöhen, …