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Showing 1 to 20 of 57 for “"N-acetylglucosamine"”.
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O-linked N-acetylglucosamine in differentiation and gene expression of mouse and human pluripotent stem cells
… cellular functions. The addition of O-linked Nacetylglucosamine to serine or threonine residues (O-GlcNAcylation) of proteins has only recently been characterized and its overall role in cell signalling remains elusive to date. Recent studies suggest an essential role of O-GlcNAcylation on the …
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Pengaruh enzim kitinase kasar dari bakteri Pseudomonas pseudomallei dan Klebsiella ozaenae terhadap pertumbuhan, morfologi, dan kadar N-asetilglukosamin Fusarium oxysporum
… of both on growth, morphology, and levels of N-acetylglucosamine F.oxysporum. This study was an experimental study using a completely randomized design (CRD), 4 treatments and 6 replications. The research was done in March to June 2013 in the Laboratory of Microbiology, Genetics, and Optical …
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Development of New Photocrosslinking Approaches to Discover Binding Partners of O-GlcNAc-Modified Proteins
O-linked β-N-acetylglucosamine (O-GlcNAc) is an abundant post-translational modification that is regulated by two enzymes, O-GlcNAc transferase (OGT) and O-GlcNAc hydrolase (OGA). While it is elusive how O-GlcNAc alters protein function, altered O-GlcNAc levels are associated with human diseases. …
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Biofilm formation on metal surfaces
… obtained the involvement of glucose and N-acetylglucosamine, present in O-antigenic fractions of LPS, in the initial attachment of the two bacterial species to mild steel is suggested. Both types of carbohydrates are likely to be involved in early attachment of Pseudomonas to mild steel, …
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Pasteurella multocida biofilm formation, and the interrelationship of P. multocida with Histophilus somni in a polymicrobial biofilm during bovine respiratory disease
… production (determined by uronic acid and N-acetylglucosamine assays), and was confirmed with capsule-deficient mutants of mucoid strains. Capsule-deficient mutants formed biofilms with a larger biomass that was much thicker and smoother than encapsulated strains. Gas chromatography-mass …
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Optimization of Fermentation Conditions for the Production of Legionaminic Acid in Recombinant Escherichia Coli
… (DO) content, induction optical density and N-acetylglucosamine (GlcNAc) feed rate on the production of legionaminic acid. Overall, results showed that the titer, yield and productivity for legionaminic acid production achieved relatively high levels, which were 5.53 g/L, 73.29% and 0.092 …
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Biochemical characterization of five GH130-family enzymes from Caldanaerobius polysaccharolyticus ATCC BAA-17 and insights on their metabolic role and reaction mechanisms
… end of substrates and mannose, glucose or N-acetylglucosamine residues, with the subsequent release of α-mannose-1-phosphate. In this study, we compare five different GH130 enzymes (CpMan130 A-E) encoded within the genome of Caldanaerobius polysaccharolyticus, a thermophilic anaerobic …
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Genetic encoding of a stable <i>O</i>-GlcNAc analogue
Attachment of N-acetylglucosamine to hydroxyl groups of serine and threonine residues of intracellular proteins is known as <i>O</i>-GlcNAcylation, an essential posttranslational event in mammals and most other metazoa, catalysed by a unique <i>O</i>-GlcNAc transferase (OGT) and removed by an …
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Immuno-gluorescence study of five zygomycetous fungi with two chitin-binding probes
… was compared to localization of oligomers of N-acetylglucosamine with the lectin wheat germ agglutinin (WGA). Dotimmunoblot and Western blot techniques revealed that the anti-serum reacted strongly with the antigen from which it was derived. Cross reactivity of the antiserum was found with WGA …
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A comparative study of in vitro chitin synthase activity in mucoraceous hosts of a mycoparasite
… the incorporation of the substrate [14C]-UDP-N-acetylglucosamine, into chitin. Most activity occurred in mixed membrane fraction. Inhibition of activity with Polyoxin D and activation with proteases, N-acetyl-glucosamine and magnesium and other ions was observed. Properties of the crude enzyme …
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Acanthamoebal surface properties and the modulation of phagocytosis
… trophozoite and cyst surfaces to be rich in N-acetylglucosamine, N-acteylneuraminic acid, mannose and glucose, with the addition of N-acetylgalactosamine on cysts. The importance of such surface properties was investigated with respect to phagocytosis of polystyrene latex microspheres, of …
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Solution and solid phase synthesis of N,N'-diacetyl chitotetraoses
… gained importance recently. The β-(1→4) linked N-acetylglucosamine (GlcNAc) moiety is a frequently occurring structural unit in various naturally and biologically important oligosaccharides and related conjugates. Chitin which is the most abundant polymer of GlcNAc is widely distributed in nature …
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Role of VimF in Gingipain Maturation in Porphyromonas gingivalis
… for rVimF was observed using UDP-galactose and N-acetylglucosamine as donor and acceptor substrates, respectively. Further, in the presence of rVimF and UDP-galactose, a 60 kDa protein from the extracellular fraction of FLL95 which was identified my mass spectrometry as Rgp gingipain, …
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Characterization of Peptidoglycan, and the Enzymes that Synthesize it, in Borrelia burgdorferi and Insights into the Peptidoglycan of Other Pathogenic Borrelia
… bacteria. It is composed of glycan strands of N-acetylglucosamine (GlcNAc) and N-acetylmuramic acid (MurNAc) crosslinked by peptide chains of alternating D- and L- amino acids and diamines. PG plays an important role in 1) cell elongation and division, 2) cell strength and morphology, 3) …
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Characterisation of the Helix pomatia agglutinin binding glycoproteins of colorectal cancer cell lines and tissue samples
… and the monosaccharides N-acetylgalactosamine, N-acetylglucosamine, and sialic acid were used to inhibit the interaction between the lectin and the cancer cells. A proteomic approach based on cell membrane isolation, pre-fractionation using lectin affinity chromatography, followed by 2-dimensional …
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Developing O-GlcNAc transferase inhibitors - insights from substrate recognition
… of nucleocytoplasmic proteins (> 1000) with -N-acetylglucosamine (O-GlcNAc / O-GlcNAcylation) is a highly dynamic and essential process in metazoa. The attachment and removal of the sugar residue is regulated by two antagonistic enzymes. The O-GlcNAc transferase (OGT), which adds the sugar onto …
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The Development of Substrates and Inhibitors of the PNAG Biosynthetic Machinery
… deacetylated polymer of β-(1,6)-linked N-acetylglucosamine (PNAG). The protein components that are responsible for the synthesis, modification and export of the PNAG polymer are encoded by the ica and pga operons in S. epidermidis and E. coli respectively. It has been shown that deletions …
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Biofilm-related Materials as Total N-nitrosamine (TONO) Precursors and Hydroxylamine-based Interferences in TONO and N-nitrosodimethylamine (NDMA) Measurements
… Three biofilm-derived materials - poly-N-acetylglucosamine (PNAG), Pseudomonas aeruginosa, and tryptophan - were individually chloraminated and dose-response relationships were observed with the SPE-TONO assay, indicating biofilm are potential N-nitrosamine precursors. The role of …
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Function and inhibition of the mitochondrial O-GlcNAc transferase isoform
The O-linked N-acetylglucosamine post-translational modification (O-GlcNAcylation) is the dynamic and reversible attachment of N-acetylglucosamine to serine and threonine residues of target proteins. It is abundant in metazoa, involving hundreds of proteins linked to a plethora of biological …
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