Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"N-WASp"”.
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Processive Acceleration of Actin Barbed End Assembly by N-WASP
… Neural Wiskott Aldrich syndrome protein (N-WASP) is involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. N-WASP activated actin polymerization drives extension of invadopodia and podosomes into the basement layer. In addition to activating Arp2/3 …
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Structural and biochemical insight into the interactions of Cdc42 with TOCA1 and N-WASP
… proteins of the Wiskott-Aldrich Syndrome (WASP) family and the Transducer of Cdc42-dependent Actin assembly (TOCA) family. The WASP family and their activation by Cdc42 have been thoroughly studied in vitro and are well understood. Conversely, understanding of the TOCA family remains …
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Investigating the Function of an Actin Nucleation-Promoting Factor N-WASP in Mammalian Myoblast Fusion
Pages 61-89 are misnumbered as pages 75-103, and pages 90-100 are misnumbered as pages 102-112.
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Effector-driven actin reorganisation restricts Salmonella invasion.
… Cdc42, which leads to the mobilisation of N-WASP and the resulting Arp2/3 complex-mediated actin assembly. This work establishes that the activity of SopE2 restricts excessive Salmonella uptake via the generation of actin structures that exhibit anti-phagocytic properties. The loss of SopE2 …
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Cip4 and Src In Promoting The Migration and Invasion of Breast Cancers
… membranes to scaffold complexes of Cdc42 and N-WASp. In these experiments, I show that CIP4 overexpression correlates with triple-negative biomarker status, cellular migration, and invasion of (breast cancer cells. Inhibition of CIP4 expression significantly decreases migration and invasion. …
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Biochemical Reconstitution and Functional Characterization of the WAVE Regulatory Complex
Members of the Wiskott-Aldrich syndrome protein (WASP) family (WASP, N-WASP, WAVE 1-3) have a central role in the transmission of the extracellular signals to the actin cytoskeleton. These proteins use their C-terminal VCA domain to stimulate the actin-nucleating activity of Arp2/3 complex in …
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Phase Transitions of Multivalent Adaptor Proteins
… interacts with its partners Nck and N-WASP in a multivalent fashion. I show here that these interactions create large assemblies that phase separate into liquid droplets, both in solution and on membranes. I also find that the creation of these assemblies affects the downstream …
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Spatiotemporal Control of Actin Cytoskeletal Machinery in Cells: Multivalency Mediated Protein Clustering at the Plasma Membrane and Optogenetic Tool Development
… interactions among proteins in the Nephrin/Nck/N-WASP actin-regulatory pathway can drive clustering of the components at the plasma membrane in cells. Generation of the micronsized domains is dependent on the phosphorylation-mediated interaction between the transmembrane protein, Nephrin, and its …
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Toca-1 driven actin polymerisation at membranes
… previously identified Toca-1 binding partner N-WASP was not. This further supports the importance of Toca-1 oligomerisation in actin polymerisation. I tested these interactions in the FLS system and found that increasing Toca-1 concentration leads to increased recruitment of N-WASP, as well as …
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Regulation of Endocytosis of ROMK Channel by WNK Kinase Family
… of dynamin and the actin polymerization of N-WASP, and thus promote endocytosis of ROMK channel. WNK1 inhibition of ROMK is further regulated by the kinase domain conformation, which is critical for WNK1 to recruit intersectin. A shorter renal alternatively spliced form of WNK1 that lacks the …
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Self-assembly of filopodia-like structures on supported lipid bilayers
… regions. BAR domain protein toca-1 recruits N-WASP then the Arp2/3 complex and actin assembly follow. Elongation proteins Diaphanous-related formin, VASP and fascin are recruited later. The Arp2/3 complex is absolutely required for FLS initiation but is not required for elongation, which may …
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Characterization of NKG2Dmediated signaling pathways and its role in the regulation of cell migration
… la proteína del síndrome de Wiskott-Aldrich (WASp) y N-WASp están implicadas en la regulación de la migración de células NK mediada por NKG2D. En células T CD8+, Rac1 se activa tras la estimulación de CD3 y CD28. Sin embargo, sólo la activación de CD3 y NKG2D incrementa adicionalmente la …
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Spatio-temporal properties of membrane-localized actin nucleating complexes
… of seven actin regulatory factors (Toca-1, N-WASP, GTPase-binding domain, Ena, VASP, Diaph3, Fascin) revealed that the FLS tip complex has both dynamic and stable properties, with different proteins displaying distinct dynamics. Further analyses on the membrane-binding protein Toca-1 showed …
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Actin polymerization dynamics at the leading edge
… beads or thin glass nanofibers coated with N-WASP WCA domains. During sustained motility, physiological concentrations of Mg²⁺ generated actin filament bundles that processively attached to the nanofiber. Reduction of total Mg²⁺ abolished particle motility and actin attachment to the particle …